Skip to main content

Development of Media for Isolation and Cultivation of Human Cancer Cells

  • Chapter
Human Tumor Cells in Vitro

Abstract

The advances in our knowledge of the growth, nutrition, and metabolic requirements of established tissue cell cultures (Rothblat and Cristofalo, 1972) have not solved the problems of developing permanent cell cultures from human solid tumor tissues. There has been little progress in our knowledge of the mechanisms by which cancer cells can become adapted to survive in vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aaronson, S. A., Todaro, G. J., and Freeman, A. E., 1970, Human sarcoma cells in culture: Identification by colony-forming ability on monolayers of normal cells, Exp. Cell Res. 61: 1.

    PubMed  CAS  Google Scholar 

  • Allison, A. C., and Law, L. W., 1968, Effects of antilymphocyte serum on virus oncogenesis, Proc. Soc. Exp. Biol. Med. 127: 207.

    PubMed  CAS  Google Scholar 

  • Anderson, N. G., 1953, The mass isolation of whole cells from rat liver, Science 117: 627.

    PubMed  CAS  Google Scholar 

  • Anigstein, L., Anigstein, D. M., and Rennels, E. G., 1965, Effect of neonatal inoculation of thymus antiserum on growth of Sarcoma 180 in mice, Texas Rep. Biol. Med. 23: 705.

    CAS  Google Scholar 

  • Anigstein, L., Anigstein, D. M., Rennels, E. G., and O’Steen, W. K., 1966, Induced alteration of resistance to transplantable mammary adenocarcinoma in mice neonatally inoculated with rat thymus antiserum, Cancer Res. 26: 1867.

    CAS  Google Scholar 

  • Auersperg, N., 1964, Long term culture of hypodiploid human tumor cells, J. Natl. Cancer Inst. 32: 135.

    PubMed  CAS  Google Scholar 

  • Baron, S., and Rabson, A. S., 1957, A culture strain (LAC) of human epithelial-like cells from an adenocarcinoma of the lung, Proc. Soc. Exp. Biol. Med. 96: 515.

    PubMed  CAS  Google Scholar 

  • Bassin, R. H., Plata, E. J., Gerwin, B. I., Mattern, C. F., Haapala, D. K., and Chu, E. W., 1972, Isolation of a continuous epithelioid cell line, HBT-3, from a human breast carcinoma, Proc. Soc. Exp. Biol. Med. 141: 673.

    PubMed  CAS  Google Scholar 

  • Baugh, C. L., and Tytell, A. A., 1967, Propagation of the human diploid cell WI-38 in galactose medium, Life Sci. 6: 371.

    PubMed  CAS  Google Scholar 

  • Berman, L., and Stulberg, C. S., 1956, Eight culture strains (Detroit) of human epithelial-like cells, Proc. Soc. Exp. Biol. Med. 92: 730.

    PubMed  CAS  Google Scholar 

  • Beynish-Melnick, M., Fernbach, D. J., and Lewis, R. T., 1963, Studies on human leukemia. 1. Spontaneous lymphoblastoid transformation of fibroblastic bone marrow cultures derived from leukemic and non-leukemic children, J. Natl. Cancer Inst. 31: 1311.

    Google Scholar 

  • Bichel, J., 1938, Dauerzuchtüng von leukämischen Zellen in vitro, Z. Krebsforsch. 48: 92.

    Google Scholar 

  • Burlington, H., 1959, Enzyme patterns in cultured kidney cells, Am. J. Physiol. 197: 68.

    PubMed  CAS  Google Scholar 

  • Burrows, M. T., 1910, The cultivation of tissues of the chick-embryo outside the body, DAMA 55: 2057.

    Google Scholar 

  • Cailleau, R., 1960, The establishment of a cell strain (MAC-2 I) from a mucoid adenocarcinoma of the human lung, Cancer Res. 20: 837.

    PubMed  CAS  Google Scholar 

  • Cameron, G., and Chambers, R., 1937, Neoplasm studies. III. Organization of cells of human tumors in tissue culture, Am. J. Cancer 30:115.

    Google Scholar 

  • Carrel, A., 1923, A method for the physiological study of tissues in vitro, J. Exp. Med. 38: 407.

    PubMed  CAS  Google Scholar 

  • Chang, R. S., 1954, Continuous subcultivation of epithelial-like cells from normal human tissues, Proc. Soc. Exp. Biol. Med. 87: 440.

    PubMed  CAS  Google Scholar 

  • Cobb, J. P., Walker, D. G., and Wright, J. C., 1961, Comparative chemotherapy studies on primary short term cultures of human normal, benign, and malignant tumor tissues—A five year study, Cancer Res. 21: 583.

    CAS  Google Scholar 

  • Coman, D. R., 1942, Human neoplasms in tissue culture, Cancer Res. 2: 618.

    Google Scholar 

  • Costachel, O., Fadei, L., and Badea, E., 1969, Tumor cell suspension culture on non-adhesive substratum, Z. Krebsforsch. 72: 24.

    PubMed  CAS  Google Scholar 

  • Debruyn, W. M., Korteweg, R., and Van Waveren, E. K., 1949, Transplantable mouse lymphosarcoma T86157 (M.B.) studied in vitro and at autopsy, Cancer Res. 9: 282.

    CAS  Google Scholar 

  • DeLuca, L., 1965, The use of trypsin for the determination of cellular viability, Exp. Cell Res. 40:186.

    Google Scholar 

  • Eagle, H., 1955a, The specific amino acid requirements of a human carcinoma cell (strain HeLa) in tissue culture, J. Exp. Med. 102: 37.

    CAS  Google Scholar 

  • Eagle, H., 1955b, Propagation in fluid medium of a human epidermoid carcinoma, strain K. B., Proc. Soc. Exp. Biol. Med. 89: 362.

    PubMed  CAS  Google Scholar 

  • Eagle, H., 1958–1959, Metabolic studies with normal and malignant human cells in culture, in: The Harvey Lectures, pp. 156–175, Academic Press, New York, 1960.

    Google Scholar 

  • Eagle, H., 1959, Amino acid metabolism in mammalian cell cultures, Science 130: 432.

    PubMed  CAS  Google Scholar 

  • Eagle, H., and Foley, G. E., 1958, Cytotoxicity in human cell cultures as a primary screen for the detection of anti-tumor agents, Cancer Res. 18:1017.

    Google Scholar 

  • Eagle, H., and Piez, K., 1962, The population-dependent requirement by cultured mammalian cells for metabolites which they can synthesize, J. Exp. Med. 116:29.

    Google Scholar 

  • Eagle, H., Barban, S., Levy, M., and Schulze, H. 0., 1958, The utilization of carbohydrates by human cell cultures, J. Biol. Chem. 233: 551.

    CAS  Google Scholar 

  • Ebeling, A. H., 1921, Fibrin and serum as a culture medium, J. Exp. Med. 33: 641.

    PubMed  CAS  Google Scholar 

  • Ehrmann, R. L., and Gey, G. 0., 1956, The growth of cells on a transparent gel of reconstituted rat-tail collagen, J. Natl. Cancer Inst. 16:1375.

    Google Scholar 

  • Evans, V. J., and Earle, W. R., 1947, The use of perforated cellophane for the growth of cells in tissue culture, J. Natl. Cancer Inst. 8: 103.

    PubMed  CAS  Google Scholar 

  • Evans, V. J., Bryant, J. C., Fioramonti, M. D., McQuilkin, W. T., Sanford, K. K., and Earle, W. R., 1956, Studies of a nutrient media for tissue cells in vitro, I. A protein-free chemically defined medium for cultivation of strain L cells, Cancer Res. 16:77.

    Google Scholar 

  • Evans, V. J., Bryant, J. C., Kerr, H. A., and Shilling, E. L., 1964, Chemically defined media for cultivation of long-term cell strains from four mammalian species, Exp. Cell Res. 36: 439.

    Google Scholar 

  • Fawcett, D. W., and Vallee, B. L., 1952, Studies on the separation of cell types in serosanguinous fluids, blood, and vaginal fluids by flotation on bovine plasma albumin, J. Lab. Clin. Med. 39: 354.

    PubMed  CAS  Google Scholar 

  • Fawcett, D. W., Vallee, B. L., and Soule, M. H., 1950, A method for concentration and segregation of malignant cells from bloody, pleural and peritoneal fluids, Science 111: 34.

    PubMed  CAS  Google Scholar 

  • Feller, W. F., Stewart, S. E., and Kantor, J., 1972, Primary tissue culture expiants of human breast cancer, J. Natl. Cancer Inst. 48:1117.

    Google Scholar 

  • Ferrebee, J. W., and Geiman, Q. M., 1946, Studies on malarial parasites. III. A procedure for preparing concentrates of Plasmodium vivax, J. Infect. Dis. 78:173.

    Google Scholar 

  • Fjelde, A., 1955, Human tumor cells in tissue culture, Cancer 8: 845.

    PubMed  CAS  Google Scholar 

  • Fogh, J., and Lund, R., 1957, Continuous cultivation of epithelial-cell strain (FL) from human amniotic membrane, Proc. Soc. Exp. Biol. Med. 94: 532.

    PubMed  CAS  Google Scholar 

  • Foley, J. F., 1973, Acid mucopolysaccharide production of human fibroblasts in vitro, presented at Central States Branch Tissue Culture Association Meeting, Ardmore, Okla., May.

    Google Scholar 

  • Foley, J. F., and Aftonomos, 1965, Growth of human breast neoplasms in cell culture, J. Natl. Cancer Inst. 34: 217.

    PubMed  CAS  Google Scholar 

  • Foulds, L., 1954, The experimental study of tumor progression: A review, Cancer Res. 14: 327.

    PubMed  CAS  Google Scholar 

  • Frisch, A. W., Jentoft, V., Burger, R., and Losli, E. J., 1955, A human epithelial-like cell (MABEN) derived from an adenocarcinoma of lung: Isolation, continuous propagation and effects of selected viruses, Am. J. Clin. Pathol. 25: 1107.

    CAS  Google Scholar 

  • Gaugas, J. M., Chesterman, F. C., Hirsch, M. S., Rees, R. J. W., Harvey, J. J., and Gilchrist, C., 1969, Unexpected high incidence of tumors in thymectomized mice treated with antilymphocytic globulin and Mycobacterium leprae, Nature (Lond.) 222: 1033.

    Google Scholar 

  • Gey, G. 0., 1933, An improved technique for massive tissue culture, Am. J. Cancer 17: 752.

    Google Scholar 

  • Gey, G. 0., 1954–1955, Some aspects of the constitution and behaviour of normal and malignant cells contained in continuous culture, in: The Harvey Lectures, Series L, pp. 154–229, Academic Press, New York, 1956.

    Google Scholar 

  • Gey, G. O., and Gey, M. K., 1936, The maintenance of human normal cells and tumor cells in continuous culture. I. Preliminary report: Cultivation of mesoblastic tumors and normal tissue and notes on methods of cultivation, Am. J. Cancer 27: 45.

    Google Scholar 

  • Gey, G. O., Coffman, W. D., and Kubicek, W. T., 1952, Tissue culture studies of the proliferate capacity of cervical carcinoma and normal epithelium, Cancer Res. 12: 264.

    Google Scholar 

  • Ginsberg, H. S., 1957, Discussion, Properties acquired by cells maintained in continuous culture, in: Cellular Biology, Nucleic Acids, and Viruses, Ann. N.Y. Acad. Sci. Spec. Publ. V, pp. 362–363.

    Google Scholar 

  • Gwatkin, R. B. L., and Thomsen, J. L., 1964, A new method for dispersing the cells of mammalian tissues, Nature (Lond.) 201: 1242.

    CAS  Google Scholar 

  • Haff, R. F., and Swim, H. E., 1956, Amino acid requirements of rabbit fibroblasts, strain RM3, Fed. Proc. 15: 591.

    Google Scholar 

  • Ham, R. G., 1963, Albumin replacement by fatty acids in clonal growth of mammalian cells, Science 140: 802.

    PubMed  CAS  Google Scholar 

  • Ham, R. G., 1965, Clonal growth of mammalian cells in a chemically defined, synthetic medium, Proc. Natl. Acad. Sci. 53: 288.

    PubMed  CAS  Google Scholar 

  • Hanks, J. H., and Wallace, J. H., 1958, Determination of cell viability, Proc. Soc. Exp. Biol. Med. 98: 188.

    PubMed  CAS  Google Scholar 

  • Harrison, R. G., 1907, Observations on the living developing nerve fiber, Proc. Soc. Exp. Biol. Med. 4: 140.

    Google Scholar 

  • Henle, G., and Deinhardt, F., 1957, The establishment of strains of human cells in tissue culture, J. Immunol. 79: 54.

    PubMed  CAS  Google Scholar 

  • Hertz, R., 1959, Choriocarcinoma of women maintained in serial passage in hamsters and rats, Proc. Soc. Exp. Biol. Med. 102: 77.

    PubMed  CAS  Google Scholar 

  • Hibbs, J. B., Lambert, L. H., and Remington, J. S., 1972, Control of carcinogenesis: A possible role for the activated macrophage, Science 177: 998.

    PubMed  Google Scholar 

  • Hinz, R. W., and Syverton, J. T., 1959, Mammalian cell cultures for the study of influenza virus. I. Preparation of monolayer cultures with collagenase, Proc. Soc. Exp. Biol. Med. 101:19. Houba, V., 1967, The use of pronase for dispersing cells, Experientia 23: 372.

    Google Scholar 

  • Hsu, T. C., and Kellog, D. S., 1960, Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens, J. Natl. Cancer Inst. 25: 221.

    PubMed  CAS  Google Scholar 

  • Ioachim, H. L., 1970, Tissue culture of human tumors: Its use and prospects, Pathol. Ann. 5: 217.

    CAS  Google Scholar 

  • Jensen, F. C., and Castellano, G. A., 1960, The long term maintenance of tumor explants, Cancer Chemother. Rep. 8:135.

    Google Scholar 

  • Jensen, F. C., Gwatkin, R. B. L., and Biggers, D., 1964, A simple organ culture method which allows simultaneous isolation of specific types of cells, Exp. Cell Res. 34: 440.

    PubMed  CAS  Google Scholar 

  • Jordan, W., 1956, Human nasal cells in continuous cultures; establishment of two lines of epithelial-like cells, Proc. Soc. Exp. Biol. Med. 92: 867.

    PubMed  Google Scholar 

  • Kalant, H., and Young, F., 1957, Metabolic behaviour of isolated liver and kidney cells, Nature (Lond.) 179: 816.

    CAS  Google Scholar 

  • Kaltenbach, J. P., 1954, The preparation and utilization of whole cell suspensions obtained from solid mammalian tissues, Exp. Cell Res. 7: 568.

    PubMed  CAS  Google Scholar 

  • Kohler, P. 0., Bridson, W. E., Hammond, J. M., Weintraub, F., Kirschner, M. A., and Van Thiel, D. H., 1971, Clonal lines of human choriocarcinoma cells in culture, in: In vitro Methods in Reproductive Cell Biology, p. 137, Karolinska Symposia on Research Methods in Reproductive Endocrinology, 3rd Symposium.

    Google Scholar 

  • Kraemer, P. M., 1972, Complex carbohydrates of mammalian cells in culture, in: Growth, Nutrition, and Metabolism of Cells in Culture, Vol. 1 ( G. H. Rothblat, and V. J. Cristofalo, eds.), pp. 371–426, Academic Press, New York.

    Google Scholar 

  • Lasfargues, E. Y., 1957, Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse, Exp. Cell Res. 13: 553.

    PubMed  CAS  Google Scholar 

  • Lasfargues, E. Y., and Moore, D. H., 1971, A method for the continuous cultivation of mammary epithelium, In vitro 7: 21.

    PubMed  CAS  Google Scholar 

  • Lasfargues, E. Y., and Ozzello, L., 1958, Cultivation of human breast carcinomas, J. Natl. Cancer Inst. 21: 1131.

    Google Scholar 

  • Lasfargues, E. Y., Coutinho, W. G., and Moore, D. H.,1972, Pitfalls in the isolation of a human breast carcinoma virus in tissue culture, J. Natl. Cancer Inst. 48: 1101.

    Google Scholar 

  • Lata, G., and Reinerston, J., 1957, Cholesterol synthesis by isolated rat liver cells, Nature (Land.) 178: 47.

    Google Scholar 

  • Laws, J., and Strickland, L., 1956, Metabolism of isolated liver cells, Nature (Land.) 178: 309.

    CAS  Google Scholar 

  • Lazarus, H., Tegeler, W., Mazzono, H. M., Leroy, J. G., Boone, B. A., and Foley, G. E., 1966, Determination of sensitivity of individual biopsy specimens to potential inhibitory agents: Evaluation of some explant culture methods as assay systems, Cancer Chemother. Rep. 50: 543.

    PubMed  CAS  Google Scholar 

  • Leibovitz, A., 1963, The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere, Am. J. Hyg. 78: 173.

    PubMed  CAS  Google Scholar 

  • Leibovitz, A., McCombs, W. B., III, Johnston, D., McCoy, C. E., and Stinson, J. C., I973a, Two new human cancer cell lines, presented at Tissue Culture Association Meeting, Boston.

    Google Scholar 

  • Leibovitz, A., McCombs, W. B., III, Johnston, D., McCoy, C. E., and Stinson, J. C., 1973b, New human cancer cell culture lines. I. SW-13, small cell carcinoma of the adrenal cortex, J. Natl. Cancer Inst. 51: 691.

    PubMed  CAS  Google Scholar 

  • Leighton, J., 1954, Studies on human cancer using sponge matrix tissue culture. I. The growth patterns of a malignant melanoma, adenocarcinoma of the parotid gland, papillary adenocarcinoma of the thyroid gland, adenocarcinoma of the pancreas, and epidermoid carcinoma of the uterine cervix (Gey’s HeLa strain), Texas Rep. Biol. Med. 12: 847.

    CAS  Google Scholar 

  • Leighton, J., 1957a, Appraisal of the malignant properties of human cells in continuous culture: A discussion, in: Cellular-Biology, Nucleic Acids, and Viruses, Ann. N.Y. Acad. Sci. Spec. Publ. V, pp. 356–361.

    Google Scholar 

  • Leighton, J., 1957b, Contributions of tissue culture studies to the understanding of the biology of cancer: A review, Cancer Res. 17: 929.

    PubMed  CAS  Google Scholar 

  • Leighton, J., Kline, I., Belkin, M., Legallais, F., and Orr, H. C., 1957, The similarity in histological appearance of some human “cancer” and “normal” cell strains in sponge-matrix tissue culture, Cancer Res. 17: 359.

    PubMed  CAS  Google Scholar 

  • Levintow, L., and Eagle, H., 1961, Biochemistry of cultured mammalian cells, in: Annual Review of Biochemistry, Vol. 30 ( J. M. Luck, F. W. Allen, and G. Mackinney, eds.) pp. 605–640, Annual Reviews, Palo Alto, Calif.

    Google Scholar 

  • Lieberman, I., and Ove, P., 1959, Growth factors for mammalian cells in culture, J. Biol. Chem. 234: 2754.

    PubMed  CAS  Google Scholar 

  • Ling, C. T., Gey, G. O., and Richters, V., 1968, Chemically characterized concentrated corodies for continuous cell culture (the 7C’s media), Exp. Cell Res. 52: 469.

    PubMed  CAS  Google Scholar 

  • Lockhart, R. Z., Jr., and Eagle, H., 1959, Requirements for growth of single human cells, Science 129: 252.

    Google Scholar 

  • Mackenzie, C. G., Mackenzie, J. B., and Beck, P., 1961, The effect of pH on growth, protein synthesis, and lipid-rich particles of cultured mammalian cells, J. Biophys. Biochem. Cytol. 9: 141.

    PubMed  CAS  Google Scholar 

  • Macpherson, I., and Montagnier, L., 1964, Agar suspension culture for the selective assay of cells transformed by polyoma virus, Virology 23: 291.

    PubMed  CAS  Google Scholar 

  • McAllister, R. M., and Reed, G., 1968, Colonial growth in agar of cells derived from neoplastic and non-neoplastic tissues of children, Pediat. Res. 2: 356.

    PubMed  CAS  Google Scholar 

  • McLimans, W. F., Mount, D. T., Bogitch, S., Crouse, E. J., Harris, G., and Moore, G. E., 1966, A controlled environment system for study of mammalian cell physiology, Ann. N.Y. Acad. Sci. 139: 190.

    PubMed  CAS  Google Scholar 

  • Moore, A. E., Sabachewsky, L., and Toolan, H. M., 1955, Culture characteristics of four permanent cell lines of human cancer cells, Cancer Res. 15: 598.

    PubMed  CAS  Google Scholar 

  • Morgan, J. F., 1958, Tissue culture nutrition, Bacteriol. Rev. 22: 20.

    PubMed  CAS  Google Scholar 

  • Morton, H. J., 1970, A survey of commercially available tissue culture media, In Vitro 6:89. Moscona, A., 1952, Cell suspensions from organ rudiments of chick embryos, Exp. Cell Res. 3: 535.

    Google Scholar 

  • Neuman, R. E., and McCoy, T. A., 1956, Dual requirement of Walker carcinosarcoma 256 in vitro for asparagine and glutamine, Science 124: 124.

    PubMed  CAS  Google Scholar 

  • Neuman, R. E., and Tytell, A. A., 1960, Amino acid requirements for growth of avian lung cultures, Proc. Soc. Exp. Biol. Med. 103: 71.

    PubMed  CAS  Google Scholar 

  • Ozzello, L., and Speer, F. D., 1958, The mucopolysaccharides in the normal and the diseased breast, Am. J. Pathol. 34: 993.

    PubMed  CAS  Google Scholar 

  • Ozzello, L., Lasfargues, E. Y., and Murray, M. R., 1960, Growth promoting activity of acid muco-polysaccharides on a strain of human mammary carcinoma cells, Cancer Res. 20: 600.

    PubMed  CAS  Google Scholar 

  • Parker, R. C., 1961, Methods of Tissue Culture, 3rd ed., Hoeber, New York.

    Google Scholar 

  • Pattillo, R. A., and Gey, G. 0., 1968, The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro, Cancer Res. 28: 1231.

    CAS  Google Scholar 

  • Patuleia, M. D., and Friend, C., 1967, Tissue culture studies on murine virus-induced leukemia cells: Isolation of single cells in agar—liquid medium, Cancer Res. 27: 726.

    Google Scholar 

  • Pierce, G. B., and Johnson, L. D., 1971, Differentiation and cancer, In Vitro 7: 140.

    PubMed  CAS  Google Scholar 

  • Pious, D. A., Hamburger, R. N., and Mills, S. E., 1963, Clonal analysis of primary human cultures, Fed. Proc. 22: 382.

    Google Scholar 

  • Pomerat, C. M., Nowinski, W. W., and Rose, G. G., 1950, Tissue culture of cells from body fluids, Texas Rep. Biol. Med. 8: 521.

    CAS  Google Scholar 

  • Puck, T., and Marcus, P. I., 1955, A rapid method for viable cell titration in tissue culture: The use of X-irradiated cells to supply conditioning factors, Proc. Natl. Acad. Sci. 41: 432.

    PubMed  CAS  Google Scholar 

  • Puck, T., Marcus, P. I., and Cieciura, S. J., 1956, Clonal growth of mammalian cells in vitro: Growth characteristics of colonies from single HeLa cells with and without a feeder layer, J. Exp. Med. 103: 653.

    CAS  Google Scholar 

  • Reed, M. V., and Gey, G. 0., 1962, Cultivation of normal and malignant lung tissue: The establishment of three adenocarcinoma cell strains, Lab. Invest. 11: 638.

    CAS  Google Scholar 

  • Richardson, H. B., and Leibovitz, A., 1965, Hand, foot, and mouth disease in children, J. Pediat. 67: 6.

    PubMed  Google Scholar 

  • Rightsel, W. A., Schultz, P., Muething, D., and McLean, I. W., 1956, Use of vinyl plastic containers in tissue culture for viral assays, J. Immunol. 76: 464.

    PubMed  CAS  Google Scholar 

  • Rinaldini, L. M., 1958, The isolation of living cells from animal tissues, Int. Rev. Cytol. 7: 587.

    CAS  Google Scholar 

  • Rinaldini, L. M., 1959, An improved method for the isolation and quantitative cultivation of embryonic cells, Exp. Cell Res. 16: 477.

    PubMed  CAS  Google Scholar 

  • Robertson, H. E., Brunner, K. T., and Syverton, J. T., 1955, Propagation in vitro of poliomyelitis viruses. III. pH change of HeLa cell cultures for assay, Proc. Soc. Exp. Biol. Med. 88: 119.

    PubMed  CAS  Google Scholar 

  • Rose, H. M., 1969, Adenoviruses, in: Diagnostic Procedures for Viral and Rickettsial Infections, ( E. H. Lennette, and N. J. Schmidt, eds.), pp. 205–226, American Public Health Association, New York.

    Google Scholar 

  • Rothblat, G. H., and Cristofalo, V. J. (eds.), 1972, Growth, Nutrition and Metabolism of Cells in Culture, Vols. 1 and 2, Academic Press, New York.

    Google Scholar 

  • Rous, P., and Jones, F. A., 1916, A method of obtaining suspensions of living cells from the fixed tissues and for the plating out of individual cells, J. Exp. Med. 23: 555.

    Google Scholar 

  • Royle, J. G., 1946, Some cultural and cytological characteristics of human tumors in vitro, Cancer Res. 6: 225.

    PubMed  CAS  Google Scholar 

  • Rubin, H., 1965–1966, The behavior of cells before and after virus-induced malignant

    Google Scholar 

  • transformation, The Harvey Lecture Series 61, Academic Press, New York, 1967.

    Google Scholar 

  • Sanders, F. K., and Burford, B. O., 1964, Ascites tumors from BHK 21 cells transformed in vitro by polyoma virus, Nature (Land.) 201: 786.

    CAS  Google Scholar 

  • Santesson, L., 1935, Characteristics of epithelial mouse tumor cells in vitro and tumor structures in vivo: A comparative study. Acta Pathol. Microbiol. Scand. Suppl. 24:1.

    Google Scholar 

  • Sinkovics, J. G., Dreyer, D. A„ Shirato, E., Cabiness, J. R., and Shellenberger, C. C., 1971, Cytotoxic lymphocytes. I. Destruction of neoplastic cells in cultures of human origin, Texas Rep. Biol. Med. 29: 227.

    CAS  Google Scholar 

  • Sinkovics, J. G., Cabiness, J. R., and Shullenberger, C. C., 1972, Monitoring in vitro of immune reactions to solid tumors, Frontiers Radiat. Ther. Oncol. 7: 99.

    Google Scholar 

  • Southam, C. M., 1954, Growth of human adenocarcinoma cells in tissue culture, Cancer 7: 394.

    PubMed  CAS  Google Scholar 

  • Southam, C. M., and Goettler, P. J., 1953, Growth of human epidermoid carcinoma cells in tissue culture, Cancer 6: 809.

    PubMed  CAS  Google Scholar 

  • Sumegi, I., and Rajka, G., 1972, Amyloid-like substance surrounding mammary cancer and basal cell carcinoma, Acta. Pathol. Microbial. Scand. Sect. A 80: 185.

    CAS  Google Scholar 

  • Sumegi, I., Goreczky, L., and Roth, I., 1954, Recent studies on the amyloid in malignant tumors, Acta. Morphol. Acad. Sci. Hungary 4: 463.

    CAS  Google Scholar 

  • Syverton, J. T., and McLaren, L. C., 1957, Human cells in continuous culture. I. Derivation of cell strains from esophagus, palate, liver and lung, Cancer Res. 17: 923.

    PubMed  CAS  Google Scholar 

  • Temin, H. M., Pierson, R. W., Jr., and Dulak, N. C., 1972, The role of serum in the control of multiplication of avian and mammalian cells in culture, in: Growth, Nutrition, and Metabolism of Cells in Culture, Vol. 1 ( G. H. Rothblat, and V. J. Cristofalo, eds.), pp. 49–81, Academic Press, New York.

    Google Scholar 

  • Therkelsen, A. J., 1964, “Sandwich” technique for the establishment of cultures of human skin for chromosome investigation, Acta Pathol. Microbial. Scand. 61:317.

    Google Scholar 

  • Toolan, H. W., 1953, Growth of human tumors in cortisone-treated laboratory animals: The possibility of obtaining permanently transplantable human tumors, Cancer Res. 13: 389.

    CAS  Google Scholar 

  • Toolan, W. H., 1954, Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H. S. No. 1, H. Ep. No. 1, H. Ep. No. 2, H. Ep. No. 3, and H. Emb. Rh No. 1, Cancer Res. 14: 660.

    PubMed  CAS  Google Scholar 

  • Toolan, W. H., 1957, Permanently transplantable human tumors maintained in conditioned heterologous hosts: H. Chon. No. 1, H. Ep. No. 4, and H. Ad. No. 1, Cancer Res. 17: 418.

    PubMed  CAS  Google Scholar 

  • Traub, R. N., 1972, Mechanism of immunosuppression by antilymphocyte antibody, Pathobiol. Ann. 2: 129.

    Google Scholar 

  • Tribble, H. R., and Higuchi, K., 1963, Studies on nutrition and metabolism of animal cells in serum-free medium. II. Cultivation of cells in suspension, J. Infect. Dis. 112:221.

    Google Scholar 

  • Trowel!, O. A., 1953, The action of cortisone on lymphocytes in vitro, J. Physiol. (Land.) 119: 274.

    Google Scholar 

  • Trowel], O. A., 1959, The culture of mature organs in a synthetic medium, Exp. Cell Res. 16:118.

    Google Scholar 

  • Vaage, J., 1968, A mechanical technique for obtaining high yields of viable dispersed tumor cells, Transplantation 6:137.

    Google Scholar 

  • Walker, D. G., Lyons, M. M., and Wright, J. C., 1965, Observation on primary short-term cultures of human tumors: A second 5-year study, Eur. J. Cancer 1: 265.

    PubMed  CAS  Google Scholar 

  • Waymouth, C., 1954, The nutrition of animal cells, Int. Rev. Cytol. 3:1.

    Google Scholar 

  • Waymouth, C., 1956, A serum-free nutrient solution sustaining rapid and continuous proliferation of strain L (Earle) mouse cells, J. Natl. Cancer Inst. 17: 315.

    PubMed  CAS  Google Scholar 

  • Waymouth, C., 1959, Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1), J. Natl. Cancer Inst. 22: 1003.

    PubMed  CAS  Google Scholar 

  • Waymouth, C., 1960, Growth in tissue culture, in: Fundamental Aspects of Normal and Malignant Growth ( W. W. Nowinski, ed.), pp. 546–587, Elsevier, Amsterdam.

    Google Scholar 

  • Waymouth, C., 1965, Construction and use of synthetic media, in: Cells and Tissues in Culture, Vol. 1 ( E. N. Wilmer, ed.), pp. 99–142, Academic Press, New York.

    Google Scholar 

  • Waymouth, C., 1967, Somatic cells in vitro: Their relationship to progenitive cells and to artificial milieux, Natl. Cancer Inst. Monogr. 26: 1.

    Google Scholar 

  • Waymouth, C., 1968, Tissue culture media: Animal cells, in: Metabolism ( P. L. Altman, and D. S. Dittmer, eds.), pp. 180–187, FASEB, Washington, D.C.

    Google Scholar 

  • Waymouth, C., 1972,Construction of tissue culture media, in: Growth, Nutrition and Metabolism of Cells in Culture, Vol. 1 (G. H. Rothblat, and V. J. Cristofalo, eds.), pp. 11-47,Academic Press, New York.

    Google Scholar 

  • Weinhouse, S., 1972, Glycolysis, respiration, and anomalous gene expression in experimental hepatomas: G.H.A., Clowes memorial lecture, Cancer Res. 32: 2007.

    Google Scholar 

  • Wespic, H. T., 1970, Separation of viable tumor cells from nonviable tumor cells by flotation on bovine serum albumin: A short communication, J. Natl. Cancer Inst. 45: 1031.

    Google Scholar 

  • White, P. R., 1946, Cultivation of animal tissues in vitro in nutrients of precisely known constitution, Growth 10: 231.

    Google Scholar 

  • White, P. R., 1949, Prolonged survival of excised animal tissues in vitro in nutrients of known composition, J. Cell Comp. Physiol. 34: 221.

    Google Scholar 

  • Whitescarver, J., Recher, L., Sykes, J. A., and Briggs, L., 1968, Problems involved in culturing human breast tissue, Texas Rep. Biol. Med. 26: 613.

    Google Scholar 

  • Williams, E. D., Brown, C. L., and Doniach, I., 1966, Pathological and clinical findings in a series of 67 cases of medullary cancer of the thyroid, J. Clin. Pathol. 19: 103.

    Google Scholar 

  • Wilson, B. W., and Lau, T. L., 1963, Dissociation and cultivation of chick embryo cells with an actinomycete protease, Proc. Soc. Exp. Biol. Med. 114: 651.

    Google Scholar 

  • Wolff, E., 1956a, La culture de cellules tumorales sur de explants d’organes in vitro, Experientia 12: 321.

    PubMed  CAS  Google Scholar 

  • Wolff, E., 1956b, La culture des tumeurs sur des organes embryonaires explantes in vitro, Rev. Med. ( Bruxells ) 36: 2235.

    Google Scholar 

  • Yohn, D. S., McD. Hammon, W., Atchison, R. W., and Castro, B. C., 1962, Serial heterotransplantation of human adenocarcinoma No. 1 in the cheek pouch of unconditioned adult Syrian hamsters, Cancer Res. 22: 443.

    CAS  Google Scholar 

  • Zaroff, L., Sato, G., and Mills, S. E., 1961, Single cell platings from freshly isolated mammalian tissue, Exp. Cell Res. 23: 565.

    Google Scholar 

  • Zimmerman, M., Devlin, T., and Pruss, M., 1960, Anaerobic glycolysis of dispersed cell suspensions from normal and malignant tissues, Nature (Loud.) 185: 315.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1975 Springer Science+Business Media New York

About this chapter

Cite this chapter

Leibovitz, A. (1975). Development of Media for Isolation and Cultivation of Human Cancer Cells. In: Fogh, J. (eds) Human Tumor Cells in Vitro . Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1647-4_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-1647-4_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1649-8

  • Online ISBN: 978-1-4757-1647-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics