Summary
PKC activity was measured during limb regeneration of Pleurodeles both in blastemata and in the central nervous system (CNS). We showed that specific PKC activity increased post-amputation until 14 days both in membrane and cytosolic compartments. A partial translocation from cytosol to membrane occurred at 14 days postamputation; denervation prevented this translocation only in blastema mesenchyme but co-culture of spinal ganglion and blastemata stimulated it. Limb regeneration, or denervation of non amputated limb had few effects on specific PKC activity in CNS but increased significantly translocation from cytosol to membrane both in spinal cord and brain. The results are discussed with regard to the control of blastema cell proliferation by nerves and the influence of nerve regeneration on translocation of PKC in CNS.
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Abbreviations
- BNTF:
-
blastema neurotrophic factor
- CNS:
-
central nervous system
- EGTA:
-
ethyleneglycol-bis (b amino ethyl ether) N′,-tetraacetic acid
- NdBGF:
-
nerve derived blastema growth factor
- NTF:
-
neurotrophic factor
- pa:
-
post amputation
- PKC:
-
protein kinase C
- PMSF:
-
phenylmethysulfonyl fluoride
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© 1989 Plenum Press, New York
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Oudkhir, M., Martelly, I., Castagna, M., Moraczewski, J., Boilly, B. (1989). Protein Kinase C Activity During Limb Regeneration of Amphibians. In: Kiortsis, V., Koussoulakos, S., Wallace, H. (eds) Recent Trends in Regeneration Research. NATO ASI Series, vol 172. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9057-2_7
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DOI: https://doi.org/10.1007/978-1-4684-9057-2_7
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