Cytochrome Oxidase and Urate Oxidase as Intracellular O2 Indicators in Studies of O2 Gradients During Hypoxia in Liver

Sies, Helmut

doi:10.1007/978-1-4684-8890-6_75

Helmut Sies⁴

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 94))

24 Accesses
13 Citations

Summary

Results obtained from a new approach of investigating O₂ distribution in intact perfused liver under hypoxic conditions are presented. Based on the signals observed by organ absorbance spectrophotometry from two compartments with oxidases of markedly different O₂ sensitivity, the mitochondria and the peroxisomes, a distribution between high O₂ and zero O₂ zones is postulated, an intermediate border zone of O₂ concentrations between the K_0.5(O₂) values being virtually absent(steep intercellular O₂ gradients).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Chapter: USD 29.95; Price excludes VAT (USA)

eBook: USD 39.99; Price excludes VAT (USA)

Softcover Book: USD 54.99; Price excludes VAT (USA)

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

References

Chance, B. & Williams, G.R. (1956) Adv. Enzymol. 17, 65.
CAS Google Scholar
Chance, B., Oshino, N., Sugano, T. & Mayevsky, A. (1973) In: Oxygen Transport to Tissue (eds. Bicher, H.I. & Bruley, D.F.) p.277, Plenum, N.Y.
Chapter Google Scholar
Chance, B. (1976) Circulation Res. Suppl. I, 38, 69.
Google Scholar
Kessler, M., Hoper, J., Krumme, B.A. (1976) Anesthesiology, 45, 184.
Article CAS Google Scholar
Klingenberg, M. (1963) Angew. Chem. 75, 900.
Article CAS Google Scholar
Loud, A.V. (1968) J. Cell Biol. 37, 27.
Article CAS Google Scholar
Lübbers, D.W. (1968) In:Biochemie des Sauerstoffs (eds. Hess, B. & Staudinger, H.J.) p.67, Springer Verlag, Heidelberg.
Chapter Google Scholar
Oshino, N., Chance, B., Sies, H., Bücher, T. (1973)Arch.Bioch.Bioph. 154, 117.
Article CAS Google Scholar
Oshino, N., Jamieson, D. & Chance, B. (1975) Biochem.J. l46, 53.
Article Google Scholar
Sies, H., Brauser, B. & Bücher, T. (1969) FEBS Lett. 5, 319.
Article CAS Google Scholar
Sies, H. (1977a) In: Tissue Hypoxia and Ischemia (eds. Reivich, M., Coburn, R., Lahiri, S. & Chance, B.) p.51, Plenum Press, New York.
Chapter Google Scholar
Sies, H. (1977b) Hoppe-Seyler’s Z. Physiol. Chem. 358, 1021.
Article CAS Google Scholar
Wilson, D.F., Stubbs, M., Oshino, N., Erecinska, M. (1974) Biochemistry 13, 5305.
Article CAS Google Scholar
Wilson, D.F., Erecinska, M., Drown, C. & Silver, I.A. (1977) Amer. J. Physiol. In press.
Google Scholar

Download references

Author information

Authors and Affiliations

Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, Germany
Helmut Sies

Authors

Helmut Sies
View author publications
You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

University of Bristol, Bristol, England
I. A. Silver
University of Pennsylvania, Philadelphia, Pennsylvania, USA
M. Erecińska
Roswell Park Memorial Institute, Buffalo, New York, USA
H. I. Bicher

Rights and permissions

Reprints and permissions

Copyright information

About this chapter

Cite this chapter

Sies, H. (1978). Cytochrome Oxidase and Urate Oxidase as Intracellular O₂ Indicators in Studies of O₂ Gradients During Hypoxia in Liver. In: Silver, I.A., Erecińska, M., Bicher, H.I. (eds) Oxygen Transport to Tissue — III. Advances in Experimental Medicine and Biology, vol 94. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8890-6_75

Download citation

DOI: https://doi.org/10.1007/978-1-4684-8890-6_75
Published: 16 April 2012
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4684-8892-0
Online ISBN: 978-1-4684-8890-6
eBook Packages: Springer Book Archive

Publish with us

Policies and ethics