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General Purpose Enumeration and Isolation Media

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Methods for the Mycological Examination of Food

Abstract

This chapter contains results of studies done to evaluate media that may be useful for routine enumeration and isolation of fungi from foods. Prior to the workshop, questionnaires were sent to twenty-five laboratories to determine which media were used for enumeration of fungi in foods. A total of fifteen different media were reported to be routinely used. The media most often used were DRBC, RBC, PDA and OGY. Likewise, recommendations in texts as to the medium of choice for routine culture of foodborne fungi vary widely. Some recommended media are not optimal for enumeration or recovery of fungi from foods, e.g., acidified PDA (APDA). Thus, several scientists were asked to compare the media they routinely used with DRBC, DG18 or OGY. The following papers report the results of these studies and others describing media for general purpose enumeration and isolation of fungi from foods.

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References

  • ANON. 1982 International Organization for Standardization. Milk and Milk-Products-Enumeration of Yeasts and Moulds (Colony Count Technique at 25°C). ISO/DIS-6611.

    Google Scholar 

  • ANON. 1983 International Organization for Standardization. Microbiology-General Guidance for the Preparation of Dilutions for Microbiological Examination. I80–6887.

    Google Scholar 

  • BAGGERMAN, W. I. 1981 A modified rose bengal medium for the enumeration of yeasts and moulds from foods. European Journal of Applied Microbiology and Biotechnology 12, 242–247.

    Article  CAS  Google Scholar 

  • DIJKHANN, K., KOOPHANS, H. & MOSSEL, D. A. A. 1979 The recovery and identification of psychrotrophic yeasts from chilled and frozen comminuted fresh meats. Journal of Applied Bacteriology 47, 4.

    Google Scholar 

  • DIJKHANN, K. E. 1982 The optimal medium-always the best choice? In Quality Assurance and Quality Control of Microbiological Culture Media. Proceedings of the IUMS-ICFHH Symposium held on 6th and 7th September 1979, in Calas de Mallorca ed. Corry, J. E. L. Darmstadt, West Germany: G. I. T.-Verlag Ernst Giebeler.

    Google Scholar 

  • HOCKING, A. D. & PITT, J. I. 1980 Dichloran-glycerol medium for enumeration of xerophilic fungi from low moisture foods. Applied and Environmental Microbiology 39, 488–492.

    CAS  Google Scholar 

  • JARVIS, B. 1973 Comparison of an improved rose bengal chlortetracycline agar with other media for selective isolation and enumeration of moulds and yeasts in foods. Journal of Applied Bacteriology 36, 723–727.

    Article  CAS  Google Scholar 

  • JARVIS, B. 1978 Methods for detecting fungi in foods and beverages. In Food and Beverage Mycology ed. Beuchat, L. R. pp. 471–504. Westport, CT: AVI Publ., Inc.

    Google Scholar 

  • JARVIS, B., SEILER, D. A. L., OULD, A. J. L. & WILLIAMS, A. P. 1983 Observations on the enumeration of moulds in food and feedingstuffs. Journal of Applied Bacteriology 55, 325–336.

    Article  CAS  Google Scholar 

  • KING, A. D., HOCKING, A. D. & PITT, J. I. 1979 Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Applied and Environmental Microbiology 37, 959–964.

    Google Scholar 

  • KOBURGER, J. A. & RODGERS, M. F. 1978 Single multiple antibiotic amended media to enumerate yeasts and molds. Journal of Food Protection 41, 367–369.

    CAS  Google Scholar 

  • MOSSEL, D. A. A., VISSER, M. & MENGERINK, W. H. J. 1962 A comparison of media for the enumeration of moulds and yeasts in foods and beverages. Laboratory Practice 11, 109–112.

    Google Scholar 

  • MOSSEL, D. A. A., VEGA, C. L. & PUT, H. M. C. 1975 Further studies on the suitability of various media containing antibacterial antibiotics for the enumeration of moulds in food and food environments. Journal of Applied Bacteriology 39, 15–22.

    Article  CAS  Google Scholar 

  • MOSSEL, D. A. A., DIJKMANN, K. E. & KOOPMANS, M. 1980 Experience with methods for the enumeration and identification of yeasts occurring in foods. In Biology and Activities of Yeasts, The Society for Applied Bacteriology Symposium Series no. 9 ed. Skinner, F. A., Passmore, S. M. & Davenport, R. R. London: Academic Press.

    Google Scholar 

  • OVERCAST, W. W. & WEAKLEY, D. J. 1969 An aureomycin rose bengal agar for enumeration of yeasts and molds in cottage cheese. Journal of Milk and Food Technology 32, 442–445.

    CAS  Google Scholar 

  • BAGGERMAN, W. I. 1981 A modified rose bengal medium for the enumeration of yeasts and moulds from foods. European Journal of Applied Microbiology and Biotechnology 12, 242–247.

    Article  CAS  Google Scholar 

  • BARNETT, H. L. & HUNTER, B. B. 1972 Illustrated Genera of Imperfect Fungi Minneapolis: Burgess Publishing Co.

    Google Scholar 

  • BURGE, H. P., SOLOMON, W. R. & BOISE, J. R. 1977 Comparative merits of eight popular media in aerometric studies of fungi. Journal of Allergy and Clinical Immunology 60, 199–203.

    Article  CAS  Google Scholar 

  • GRIFFIN, G. J., FORD, R. H. & GARREN, K. H. 1975 Relation of Aspergillus flaws colony growth on three selective media to recovery from naturally infested soil. Phytopathology 65, 704–707.

    Article  Google Scholar 

  • HENSON, O. E. 1981 Dichloran as an inhibitor of mold spreading in fungal plating media: effects on colony diameter and enumeration. Applied and Environmental Microbiology 42, 656–660.

    CAS  Google Scholar 

  • HENSON, O. E., HALL, P. A., ARENDS, R. E., ARNOLD, E. A. JR., KNECHT, R. M., JOHNSON, C. A., PUSCH, D. J. & JOHNSON, M. G. 1982 Comparison of four media for the enumeration of fungi in dairy products-a collaborative study. Journal of Food Science 47, 930–932.

    Article  Google Scholar 

  • HOCKING, A. D. & PITT, J. I. 1980 Dichloran-glycerol medium for enumeration of xerophilic fungi from low-moisture foods. Applied and Environmental Microbiology 39, 488–492.

    CAS  Google Scholar 

  • KING, A. D. JR., HOCKING, A. D. & PITT, J. I. 1979 Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Applied and Environmental Microbiology 37, 959–964.

    Google Scholar 

  • KOBURGER, J. A. 1976. In Compendium of Methods for the Microbiological Examination of Foods ed. Speck, M. L. Ch. 16, pp. 225–229. Washington, D.C.: American Public Health Association.

    Google Scholar 

  • MOSSEL, D. A. A., VEGA, C. L. & PUT, H. H. C. 1975 Further studies on the suitability of various media containing antibacterial antibiotics for the enumeration of moulds in food and food environments. Journal of Applied Bacteriology 39, 15–22.

    Article  CAS  Google Scholar 

  • OTTOW, J. C. G. 1972 Rose bengal as a selective aid in the isolation of fungi and actinomycetes from natural sources. Mycologia 64, 304–315.

    Article  CAS  Google Scholar 

  • OVERCAST, W. W. & WEAKLEY, D. J. 1969 An aureomycin-rose bengal agar for enumeration of yeast and mold in cottage cheese. Journal of Milk and Food Technology 32, 442–445.

    CAS  Google Scholar 

  • RAPER, K. B. & FENNELL, D. I. 1965 The Genus Aspergillus. Baltimore: Williams and Wilkins.

    Google Scholar 

  • SOKAL, R. R. & ROHLF, F. J. 1973 Introduction to Biostatistics. San Francisco: W. H. Freeman and Company.

    Google Scholar 

  • BAGGERMAN, W. I. 1981 A modified rose bengal medium for the enumeration of yeasts and moulds from foods. European Journal of Applied Biotechnology 12, 242–247.

    Article  CAS  Google Scholar 

  • JARVIS, B. 1983 Comparison of an improved rose bengal-chlortetracycline agar with other media for selective isolation and enumeration of molds and yeasts in food. Journal of Applied Bacteriology 36, 723–727.

    Article  Google Scholar 

  • KAUFMAN, D. D., WILLIAMS, L. E. & SUMMER, C. B. 1963 Effect of plating medium and incubation temperature on growth of fungi in soil-dilution plates. Canadian Journal of Microbiology 9, 741–751.

    Article  Google Scholar 

  • PITT, J. I. 1979 The Genus Penicillium and its Teleomorphic States Eupenicillium and Talaromyces. London: Academic Press.

    Google Scholar 

  • JARVIS, B. 1973 Comparison of an improved rose bengal chlortetracycline agar with other media for the selective isolation and enumeration of moulds and yeasts in foods. Journal of Applied Bacteriology 36, 723–727.

    Article  CAS  Google Scholar 

  • JARVIS, B. 1978 Methods for detecting fungi in foods and beverages. In Food and Beverage Mycology ed. Beuchat, L. R. pp. 471–504. Westport, CT: AVI Publ., Inc.

    Google Scholar 

  • JARVIS, B., SEILER, D. A. L., OULD, A. J. L. & WILLIAMS, A. P. 1983 Observations on the enumeration of moulds in food and feedstuffs. Journal of Applied Bacteriology 55, 325–336.

    Article  CAS  Google Scholar 

  • KING, A. DOUGLAS, JR., HOCKING, A. D. & PITT, J. I. 1979 Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Applied and Environmental Microbiology 37, 959–964.

    Google Scholar 

  • KOBURGER, J. A. & FARHAT, B. Y. 1975 Fungi in foods VI. A comparison of media to enumerate yeasts and molds. Journal of Milk and Food Technology 38, 466–468.

    Google Scholar 

  • SANSING, G. A. & CIEGLER, A. 1973 Mass propagation of conidia from several Aspergillus and Penicillium species. Applied Microbiology 26, 830–831.

    CAS  Google Scholar 

  • SPECK, M. L. (ed). 1976 Compendium of Methods for the Microbiological Examination of Foods. Washington, D. C.: American Public Health Association.

    Google Scholar 

  • STEEL, R. G. & TORRIE, J. H. 1980 Principles and Procedures of Statistics, a Biometical Approach. 2nd edn. p. 235. New York: McGraw-Hill Book Co.

    Google Scholar 

  • AZZOUZ, M. A. & BULLERMAN, L. B. 1982 Comparative antimycotic effects of selected herbs, spices, plant components and commercial antifungal agents. Journal of Food Protection 45, 1298–1301.

    Google Scholar 

  • BAGGERMAN, W. I. 1981 A modified rose bengal medium for the enumeration of yeasts and moulds from foods. European Journal of Applied Microbiology and Biotechnology 121, 242–247.

    Article  Google Scholar 

  • BARNETT, H. L. & HUNTER, B. B. 1972 Illustrated Genera of Imperfect Fungi. 3rd. edn. Minneapolis: Burgess Publishing Company.

    Google Scholar 

  • BEUCHAT. L. R. 1979 Comparison of acidified and antibiotic-supplemented potato dextrose agar from three manufacturers for its capacity to recover fungi from foods. Journal of Food Protection 42, 427–428.

    Google Scholar 

  • FIRSTENBERG, R., MANNHEIM, C. H. & COHEN, A. 1974 Microbial quality of dehydrated onions. Journal of Food Science 39, 685–688.

    Article  Google Scholar 

  • HARRINGAN, W. F. & McCANCE, M. E. 1976 Laboratory Methods in Food and Dairy Microbiology. New York: Academic Press.

    Google Scholar 

  • HEATH, H. 1983 The microbiology of onion products. Food, Flavorings, Ingredients, Packaging and Processing 5(1), 22–24, 27.

    Google Scholar 

  • HENSON, O. E. 1981 Dichloran as an inhibitor of mold spreading in fungal plating media: Effects on colony diameter and enumeration. Applied and Environmental Microbiology 42, 656–660.

    CAS  Google Scholar 

  • JARVIS, B. 1973 Comparison of an improved rose bengal-chlortetracycline agar with other media for the selective isolation and enumeration of moulds and yeasts in foods. Journal of Applied Bacteriology 36, 723–727.

    Article  CAS  Google Scholar 

  • KING, A. D., HOCKING, A. D. & PITT, J. I. 1979 Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Applied and Environmental Microbiology 37, 959–964.

    Google Scholar 

  • KOBURGER, J. A. 1970 Fungi in foods. I. Effect of inhibitor and incubation temperature on enumeration. Journal of Milk and Food Technology 33, 433–434.

    Google Scholar 

  • KOBURGER, J. A. 1971 Fungi in foods. II. Some observations of acidulants used to adjust media pH for yeasts and mold counts. Journal of Milk and Food Technology 34, 475–477.

    Google Scholar 

  • KOBURGER, J. A. 1973 Fungi in foods. V. Response of natural populations to incubation temperatures between 12 and 32 C. Journal of Milk and Food Technology 36, 434–435.

    Google Scholar 

  • KOBURGER, J. A. & ROGERS, M. F. 1978 Single or multiple antibiotic-amended media to enumerate yeasts and molds. Journal of Food Protection 41, 367–369.

    CAS  Google Scholar 

  • LLEWELLYN, G. C., BURKETT, M. L. & EADIE, T. 1981. Potential mold growth, aflatoxin production and antimycotic activity of selected natural spices and herbs. Journal of Association of Official Analytical Chemists 64, 955–960.

    CAS  Google Scholar 

  • MALLOCH, D. 1981 Moulds. Their Isolation, Cultivation and Identification. Toronto: University of Toronto Press.

    Google Scholar 

  • MISLIVEC, P. B. & BRUCE, V. R. 1976 Comparison of antibiotic-amended potato dextrose agar and acidified potato dextrose agar as growth substrates for fungi. Journal of Association of Official Analytical Chemists 59, 720–721.

    CAS  Google Scholar 

  • MOSSEL, D. A. A. & SHENNAN, J. L. 1976 Microorganisms in dried foods: Their significance, limitation and enumeration. Journal of Food Technology 11, 205–220.

    Article  Google Scholar 

  • MOSSEL, D. A. A., VEGA, C. L. & PUT, H. M. C. 1975 Further studies on the suitability of various media containing antibacterial antibiotics for the enumeration of moulds in food and food environments. Journal of Applied Bacteriology 39, 15–22.

    Article  CAS  Google Scholar 

  • MURPHY, R. P. 1973 Microbiological contamination of dried vegetables. Process Biochemistry 8 (10), 17–19.

    Google Scholar 

  • NIE, N. H., HULL, C. H., JENKINS, J. G., STEINBREENER, K. & BENT, D. H. 1975. Statistical Package for Social Sciences. 2nd edn. New York: McGraw-Hill Book Co.

    Google Scholar 

  • POWERS, E. M., LAWYER, R. & MASUOKA, Y. 1975 Microbiology of processed spices. Journal of Milk and Food Technology 38, 683–687.

    Google Scholar 

  • SCHWAB, A. H., HARPESTAD, A. D., SWARTZENTRUBER, A., LANIER, J. M., WENTZ, B., A., DURAN, A. P., BARNARD, R. J. & READ, R. B., JR. 1982 Microbiological quality of some spices and herbs in retail markets. Applied and Environmental Microbiology 44, 627–630.

    CAS  Google Scholar 

  • SMITH, N. R. & DAWSON, V. T. 1944. The bacteriostatic action of rose bengal in media used for plate counts of soil fungi. Soil Science 58, 467–471.

    Article  CAS  Google Scholar 

  • TALBOT, P. H. B. 1971 Principles of Fungal Taxonomy. New York: St. Martin’s Press.

    Google Scholar 

  • U. S. FOOD AND DRUG ADMINISTRATION. 1978 Bacteriological Analytical Manual for Foods. Washington, D. C.: Food and Drug Administration, U. S.. Department of Health and Human Services.

    Google Scholar 

  • HENSON, O. E. 1981 Dichloran as an inhibitor of mold spreading in fungal plating media: Effects on colony diameter and enumeration. Applied and Environmental Microbiology 42, 656–660.

    CAS  Google Scholar 

  • HOCKING, A. D. & PITT, J. I. 1980 Dichloran-glycerol medium for enumeration of xerophilic fungi from low-moisture foods. Applied and Environmental Microbiology 39, 488–492.

    CAS  Google Scholar 

  • KING, A. D., JR., HOCKING, A. D. & PITT, J. I. 1979 Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Applied and Environmental Microbiology 37, 959–964.

    Google Scholar 

  • PITT, J. I. 1975 Xerophilic fungi and the spoilage of foods of plant origin. In Water Relations of Foods. ed. Duckworth, R. B. New York: Academic Press.

    Google Scholar 

  • PITT, J. I., CHRISTIAN, J. H. B. 1968 Water relations of xerophilic fungi isolated from prunes. Applied Microbiology 16, 1853–1858.

    CAS  Google Scholar 

  • PITT, J. I. & HOCKING, A. D. 1977 Influence of solute and hydrogen ion concentration on the water relations of some xerophilic fungi. Journal of General Microbiology 101, 35–40.

    CAS  Google Scholar 

  • RAYMAN, M. K., D’AOUST, J.-Y., ARIS, B., MAISHMENT, C. & WASIK, R. 1979 Survival of microorganisms in stored pasta. Journal of Food Protection 42, 330–334.

    Google Scholar 

  • RAYMAN, M. K., WEISS, K. F., RIEDEL, G. W., CHARBONNEAU, S. & JARVIS, G. A. 1981 Microbiological quality of pasta products sold in Canada. Journal of Food Protection 44, 746–749.

    Google Scholar 

  • SWARTZENTRUBER, A., PAYNE, W. L., WENTZ, B. A., BARNARD, R. J. & READ, R. B., JR. 1982 Microbiological quality of macaroni and noodle products obtained at retail markets. Applied and Environmental Microbiology 44, 540–543.

    CAS  Google Scholar 

  • CHRISTENSEN, C. M. 1946 The quantitative determination of molds in flour. Cereal Chemistry 23: 322–329.

    Google Scholar 

  • HOCKING, A. D. & PITT, J. I. 1980 Dichloran-glycerol medium for enumeration of xerophilic fungi from low-moisture foods. Applied and Environmental Microbiology 39, 488–492.

    CAS  Google Scholar 

  • HOCKING, A. D. 1981 Improved media for enumeration of fungi from foods. CSIRO Food Research Quarterly 41, 7–11.

    Google Scholar 

  • JARVIS, B. 1973 Comparison of an improved rose bengal-chlortetracycline agar with other media for selective isolation and enumeration of moulds in yeasts in foods. Journal of Applied Bacteriology 36, 723–727.

    Article  CAS  Google Scholar 

  • KING, A. D., HOCKING, A. D. and PITT, J. I. 1979 Dichloran-rose Bengal medium for enumeration and isolation of molds from foods. Applied and Environmental Microbiology 37, 959–964.

    Google Scholar 

  • MOSSEL, D. A. A., KLEYNEN, SEMMELING, A. M. C., VINCENTIE, H. M., BEERENS, H. & CATSARAS, H. 1970 Oxytetracycline-glucose-yeast extract agar for selective enumeration of molds and yeasts in foods and clinical material. Journal of Applied Bacteriology 33, 454–457.

    Article  CAS  Google Scholar 

  • PITT, J. I. 1983 Personal communication.

    Google Scholar 

  • STATISTICAL ANALYSIS SYSTEM 1979 SAS User’s Guide. 494 pp. SAS Institute, Inc., Cary, NC. 494 pp.

    Google Scholar 

  • DIJKMANN, K. E., KOOPMANS, M. & MOSSEL, D. A. A. 1979 The recovery and identification of psychrotrophic yeasts from chilled and frozen comminuted fresh meats. Journal of Applied Bacteriology 47, 4.

    Google Scholar 

  • DIJKMANN, K. E. 1982 The optimal medium-always the best choice? In: Quality Assurance and Quality Control of Microbiological Culture Media. Proceedings of the IUMS-ICFMH Symposium held on 6th and 7th September, 1979, in Calas de Mallorca. ed. Corry, J. E. L. pp. 175–180. Darmstadt, West Germany: G.I.T.-Verlag Ernst Giebeler.

    Google Scholar 

  • ENGEL, G. 1982 Vergleich verschiedener NährbÖden zum quantitativen Nachweis von Hefen and Schimmelpilzen in Milch and Milchprodukten. Milschwissenschaft 37, 727–730.

    Google Scholar 

  • LOWRY, P. D. & GILL, C. O. 1984 Temperature and water activity minima for the growth of spoilage moulds from meat. Journal of Applied Bacteriology 56, 193–199.

    Article  CAS  Google Scholar 

  • MOSSEL, D. A. A., VEGA, C. L. & PUT, H. M. C. 1975 Further studies on the suitability of various media containing antibacterial antibiotics for the enumeration of moulds in food and food environments. Journal of Applied Bacteriology 39, 15–22.

    Article  CAS  Google Scholar 

  • MOSSEL, D. A. A., DIJKMANN, K. E. & KOOPMANS, H. 1980 Experience with methods for the enumeration and identification of yeasts occurring in foods. In Biology and Activities of Yeasts. The Society for Applied Bacteriology Symposium Series No. 9. ed. Skinner, F. A., Passmore, S. M. & Davenport, R. R. pp. 279–288. London: Academic Press.

    Google Scholar 

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Pitt, J.I. et al. (1986). General Purpose Enumeration and Isolation Media. In: King, A.D., Pitt, J.I., Beuchat, L.R., Corry, J.E.L. (eds) Methods for the Mycological Examination of Food. NATO ASI Series, vol 122. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8453-3_2

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