Summary
Oxygen dependence of the lifetime of the excited triplet state of phosphorescent molecules can be used to measure the oxygen concentration in aqueous media. These measurements are insensitive to much of the optical interference that limits the usefulness of measurements based on the oxygen dependent quenching of luminescence intensity. The measurements also extend to significantly lower oxygen concentrations than are normally attainable using oxygen electrodes. The phosphorescence lifetimes can be accurately measured from a few microseconds to seconds, permitting a wide dynamic range of oxygen concentration measurements. With currently available probes, for example, it is possible to make continuous measurement of oxygen concentrations from 10−4 M to 10−8 M in a single experiment.
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© 1987 Plenum Press, New York
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Wilson, D.F., Vanderkooi, J.M., Green, T.J., Maniara, G., Defeo, S.P., Bloomgarden, D.C. (1987). A Versatile and Sensitive Method for Measuring Oxygen. In: Silver, I.A., Silver, A. (eds) Oxygen Transport to Tissue IX. Advances in Experimental Medicine and Biology, vol 215. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7433-6_9
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DOI: https://doi.org/10.1007/978-1-4684-7433-6_9
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