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Simultaneous Measurement of Haemoglobin Oxygenation of Brain and Skeletal Muscle of Rat in Vivo by Near-Infrared Spectrophotometry

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Oxygen Transport to Tissue IX

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 215))

Abstract

Analysing transmitted light at 700 and 730 nm, we found a linear relationship between the blood concentration and the absorbance change in perfused hindlimb of rat when physiological haematocrits (Hc) in the range of 15 to 50% were used for the inflowing perfusate. Several optical parameters, which are required for calculation of changes in oxyhaemoglobin, deoxyhaemoglobin and blood volume, were determined. In the present paper, we report the application of a two-wavelength method to the simultaneous measurement of haemoglobin oxygenation of rat brain and skeletal muscle at steady state in vivo.

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References

  • Ruderman, N.B., Houghton, C.R.S. and Hems, R. (1971). Evaluation of the isolated perfused rat hindquarter for the study of muscle metabolism. Biochem. J. 124, 639–651.

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  • Smith, J.J. and Kampine, J.P. (1980). Circulatory Physiology - The Essentials. The Williams & Wilkins Company, Baltimore, MD.

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  • Sugano, T., Suda, K., Shimada, M. and Oshino, N. (1978). Biochemical and ultrastructural evaluation of isolated rat liver systems perfused with hemoglobin-free medium. J. Biochem. (Tokyo), 83, 995–1007.

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Author information

Authors and Affiliations

  1. Research Institute of Applied Electricity, Hokkaido University, 060 Sapporo, Japan

    A. Seiyama, O. Hazeki & M. Tamura

Authors
  1. A. Seiyama
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  2. O. Hazeki
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  3. M. Tamura
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Editor information

Editors and Affiliations

  1. The Medical School, University of Bristol, Bristol, UK

    I. A. Silver  & A. Silver  & 

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© 1987 Plenum Press, New York

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Seiyama, A., Hazeki, O., Tamura, M. (1987). Simultaneous Measurement of Haemoglobin Oxygenation of Brain and Skeletal Muscle of Rat in Vivo by Near-Infrared Spectrophotometry. In: Silver, I.A., Silver, A. (eds) Oxygen Transport to Tissue IX. Advances in Experimental Medicine and Biology, vol 215. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7433-6_32

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  • DOI: https://doi.org/10.1007/978-1-4684-7433-6_32

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7435-0

  • Online ISBN: 978-1-4684-7433-6

  • eBook Packages: Springer Book Archive

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