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Tobacco Mosaic Virus Structure and Self-Assembly

  • Chapter
The Plant Viruses

Part of the book series: The Viruses ((VIRS))

Abstract

Tobacco mosaic virus (TMV) has become a classical object for studies on the structure and assembly of viruses. Shortly after the first purification of virus by Stanley (1935), structural studies were begun using the methods of biochemistry (Bawden and Pirie, 1937) and X-ray diffraction (Bernal and Fankuchen, 1941). The difficulties encountered in elucidating a structure of this size led to many developments in both techniques and instrumentation which enabled the structure to be determined at steadily increasing resolution. Since the virus has a helical structure (Watson, 1954) and forms highly ordered gels rather than single crystals, the three-dimensional structure must be determined by deconvolution from two-dimensional diffraction patterns of the virus gels which are azimuthally disordered. Notwithstanding the difficulties imposed by this, the virus structure has been solved to a resolution approaching 0.4 nm (Stubbs et al., 1977). In the absence of RNA, the protein will form true crystals of one of its aggregates (the disk; see Section II.B.1) whose structure has been solved to a resolution beyond 3 nm which allowed an atomic model to be built (Bloomer et al., 1978).

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Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, England

    A. C. Bloomer & P. J. G. Butler

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  1. A. C. Bloomer
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  2. P. J. G. Butler
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  1. Institute of Molecular and Cellular Biology, 94720, Strasbourg, France

    M. H. V. Van Regenmortel

  2. Department of Molecular Biology and Virus Laboratory, University of California, Berkeley, California, USA

    Heinz Fraenkel-Conrat

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© 1986 Plenum Press, New York

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Bloomer, A.C., Butler, P.J.G. (1986). Tobacco Mosaic Virus Structure and Self-Assembly. In: Van Regenmortel, M.H.V., Fraenkel-Conrat, H. (eds) The Plant Viruses. The Viruses. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7026-0_2

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  • DOI: https://doi.org/10.1007/978-1-4684-7026-0_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7028-4

  • Online ISBN: 978-1-4684-7026-0

  • eBook Packages: Springer Book Archive

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