Abstract
Using biochemical and immunohistochemical techniques, the biosynthesis of the excitotoxin quinolinic acid (QUIN) and the anti-excitotoxin kynurenic acid (KYNA) in the rat brain has been demonstrated to take place preferentially in glial cells (see Schwarcz and Du, this volume, for review). Although a dysfunction of either of these two brain metabolites has been hypo-thetically associated with the occurrence of human neuro-psychiatric diseases and seizure disorders (Lapin, 1981; Schwarcz et al., 1984; Stone and Burton, 1988), very little is known about the cellular localization of QUIN and KYNA metabolism in the human brain. We have now used human gliomas obtained during neurosurgery to assess the activity of three enzymes involved in QUIN biosynthesis, and have studied, in separate experiments, the ability of gli-oma to produce KYNA from its bioprecursor L-kynurenine (KYN).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Carlà, V., Lombardi, G., Bensi, M., Russi, P., Moneti, G., and Moroni, F., 1988, Identification and measurement of kynurenic acid in the rat brain and other organs, Anal. Biochem., 371: 267–277.
Foster, A.C., Okuno. E., Brougher, D.S., and Schwarcz, R., 1986a, A radio-enzymatic assay for quinolinic acid, Anal. Biochem., 158: 98–103.
Foster, A.C., White, R.J., and Schwarcz, R., 1986b, Synthesis of quinolinic acid by 3-hydroxyanthranilic acid oxygenase in rat brain tissue in vitro. J. Neurochem., 47: 23–30.
Lapin, I.L., 1981, Kynurenines and seizures, Epilepsia, 22: 257–265.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193: 265–275.
Schwarcz, R., Foster, A.C., French, E.D., Whetsell, Jr., W.O., and Köhler, C., Excitotoxic models for neurodegenerative disorders, Life Sci., 35: 19–32.
Schwarcz, R., Okuno E., White, R.J., Bird, E.D., and Whetsell, W.O. Jr., 1988, 3-Hydroxyanthranilate oxygenase activity is increased in the brains of Huntington disease victims, Proc. Natl. Acad. Sci. USA, 85: 4079–4081.
Speciale, C., and Schwarcz, R., 1990, Uptake of kynurenine into rat brain slices, J. Neurochem., 54: 156–163.
Stone, T.W., and Burton, N.R., 1988, NMDA receptors and ligands in the vertebrate CNS, Prog. Neurobiol., 10: 333–368.
Turski, W.A., Gramsbergen, J.B.P., Traitler, H., and Scharcz, R., 1989, Rat brain slices produce and liberate kynurenic acid upon exposure to L-kynurenine, J. Neurochem., 52: 1629–1636.
Turski, W.A., Nakamura, M., Todd, W.P., Carpenter, B.K., Whetsell, W.O., and Schwarcz, R., 1988, Identification and quantification of kynurenic acid in human brain tissue, Brain Res., 454: 164–169.
Vezzani, A., Gramsbergen, J.B.P., Versari, P., Stasi, M.A., Procaccio, F., and Schwarcz, R., 1990, Rynurenic acid synthesis by human glioma, J. Neurol. Sci., 99: 51–57.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Plenum Press, New York
About this chapter
Cite this chapter
Vezzani, A., Gramsbergen, J.B.P., Speciale, C., Schwarcz, R. (1991). Production of Quinolinic Acid and Kynurenic Acid by Human Glioma. In: Schwarcz, R., Young, S.N., Brown, R.R. (eds) Kynurenine and Serotonin Pathways. Advances in Experimental Medicine and Biology, vol 294. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5952-4_95
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5952-4_95
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5954-8
Online ISBN: 978-1-4684-5952-4
eBook Packages: Springer Book Archive