Abstract
Stable callus with embryogenic or organogenic potentiality leading to plant regeneration is a prerequisite for in vitro selection and genetic transformation of plants. Krul and Worley (1977) obtained callogenesis from internodes, petioles, flower parts and leaf veins of 2 Vitis vinifera cultivars and 2 interspecific hybrids using Murashige and Skoog (MS, 1962) medium supplemented with 1 mg/l 2,4-D and 0.1 mg/l 6-BAP. Also, Hawker et al. (1973) and Jona and Webb (1978) induced callogenesis in immature grape tissue. Recently, Katsirdakis and Roubelakis-Angelakis (1990) studied the quantitative and qualitative callogenic response of leaf segments in 2 Vitis vinifera cultivars and 6 Vitis spp. interspesific hybrids to 2 culture media and 48 combinations of plant growth regulators. Best results in callus quality were found at 2 or 4 µM 6-BAP in combination with 5 µM NAA. In contrary, 5 or 10 µM 2,4-D and 2 or 4 µM 6-BAP with 5 µM NAA gave callus of poor quality. NAA alone caused development of root radicles. There was a varying response among genotypes (Katsirdakis and Roubelakis-Angelakis, 1990). Maximum quantity of callus from internode expiants of an interspecific hybrid was obtained at 1/5/5 µM, BA/2,4-D/NOA, respectively (Rajasekaran and Mullins, 1981).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Alleweldt, G., and Radler, F., 1962, Inter-relationship between photoperiodic behavior of grapes and growth of plant tissue cultures. Plant Physiol., 37(3): 376–379.
Barlass, M., and Skene, K.G.M., 1980, Studies on the fragmented shoot apex of grapevine. II. Factors affecting growth and differentiation. J. Exp. Bot., 31 (121): 489–495.
Brezeanu, A., Iordan, M., and Rosu, A., 1980, The micropropagation of callus from tissue of somatic origin. Rev. Roum. Biol. Ser. Biol. Vegetale, 25: 135–142.
Goussard, P.G., 1981, Effects of cytokinins on elongation, proliferation and total mass of shoots derived from shoot apices of grapevine cultured in vitro. Vitis 20: 228–234.
Hawker, J.S., Downton, W.J.S., Wiskich, D., and Mullins, M.G., 1973, Callus and cell culture from grape berries. Hort. Science, 8(5): 398–399.
Jona, R., and Webb, K.J., 1978, Callus and axillary bud culture of Vitis vinifera Sylvaner Riesling. Sci. Hort., 9(1): 55–60.
Katsirdakis, K.C., and Roubelakis-Angelakis, 1990, Organo- and callogenic potentiality of leaf segments from Vitis spp. genotypes. J. Amer. Soc. Hort. Sci., accepted.
Ketel, D.H., Bretelez, H., and de Groot, B., 1985, Effect of expiant origin on growth and differentiation of calli from Tagetes species. J. Plant Physiol., 118: 327–333.
Krul, W.R., and Worley, J.F., 1977, Formation of adventitious embryos in callus cultures of Seyval french hybrid grape. J. Amer. Soc. Hort. Sci., 102(3): 360–363.
Krul, W.R., and Mowbray, G.H., 1984, Grapes, In: Hand Book of Plant Cell Culture, W.R. Sharp, D.A. Evans, P.V. Ammirato, and Y. Yamada, eds, Vol. 2, pp. 396–434, Acad. Press, London.
Krul, W.R., 1988, Recent advances in protoplast culture of horticultural crops: small fruits. Sci. Hortic, 37: 231–246.
Monette, P.L., 1983, Influence of size of culture vessel on in vitro proliferation of grape in a liquid medium. Plant Cell Tissue Organ Cult., 2: 327–332.
Murashige, T., and Skoog, F., 1962, A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant., 15: 473–497.
Nitsch, C., and Nitsen, J.P., 1967, The induction of flowering in vitro in stem segments of Plumbage indica L.I. The production of vegetative buds. Planta 72:355–370.
Novak, F.J., and Junova, Z., 1983, Clonal propagation of grapevine through in vitro axillary bud culture. Sci. Hort., 18:321–340.
Rajasekaran, K., and Mullins, M.G., 1981, Organogenesis in internode expiants of grapevines. Vitis 20:218–227.
Reisch, B.I., 1986, Influence of genotype and cytokinins on in vitro shoot proliferation of grapes. J. Amer. Soc. Hort. Sci., 111(1): 138–141.
Roubelakis-Angelakis, K.A., and S.A. Zivanovitch, 1990, Morphogenetic responses of grapevine (Vitis spp.) genotypes to plant growth regulators and culture media. J. Amer. Soc. Hort. Sci., accepted.
Sasahara, H., Tada, K., Irs, M., Takazawa, T., and Tazaki, M., 1981, Regeneration of plantlets by meristem tip culture for virus-free grapevine. J. Japan. Soc. Hort. Sci., 50:169–175.
Wang, Y., Ge, K., Zou, G., Yang, J., and Yel, M., 1984, Callus induction and plantlet regeneration in grapevines. Int. Symp. on Genetic Manipulation in Crops, Beijing, People’s Republic of China, Abstr., 22–26 Oct. 1984.
Yu, Danhua, and Meredith, C.P., 1986, The influence of expiant origin on tissue browning and shoot production in shoot tip cultures of grapevine. J. Amer. Soc. Hort. Sci., 111 (6): 972–975.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Plenum Press, New York
About this chapter
Cite this chapter
Roubelakis-Angelakis, K.A., Katsirdakis, K.C. (1990). In Vitro Micromultiplication of Grapevine: Effect of age, genotype and culture conditions on induction of callus in Vitis spp. leaf segments. In: Rodríguez, R., Tamés, R.S., Durzan, D.J. (eds) Plant Aging. NATO ASI Series, vol 186. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5760-5_11
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5760-5_11
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5762-9
Online ISBN: 978-1-4684-5760-5
eBook Packages: Springer Book Archive