Skip to main content

New Polymers for Aqueous Two-Phase Systems

  • Chapter
Separations Using Aqueous Phase Systems

Abstract

New aqueous polymer two-phase systems have been studied. The aim has been to develop phase systems suitable for large scale applications. Derivatives of starch, cellulose and poly(vinyl alcohol) were investigated. The phase separation in aqueous solutions of the polymers was studied, as well as the partitioning in the new phase systems of macromolecules, cell particles and cells. With cellulose derivatives it has been found that phase systems can be formed at very low polymer concentrations. In phase systems based on poly(vinyl alcohol) new partitioning properties have been obtained.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. P.-Å. Albertsson, “Partition of Cell Particles and Macromolecules,” 3rd ed., Wiley, New York (1986)

    Google Scholar 

  2. H. Walter, D.E. Brooks and D. Fisher, “Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology,” Academic Press, New York (1985)

    Google Scholar 

  3. K.H. Kroner, H. Hustedt and M.-R. Kula, Extractive enzyme recovery: economic considerations, Process Biochem. 19:170 (1984)

    Google Scholar 

  4. K.H. Kroner, H. Hustedt and M.-R. Kula, Evaluation of crude dextran as phase-forming polymer for the extraction of enzymes in aqueous two-phase systems in large scale, Biotechnol. Bioeng. 24:1015 (1982)

    Article  PubMed  CAS  Google Scholar 

  5. F. Tjerneld, I. Persson, P.-Å. Albertsson and B. Hahn-Hägerdal, Enzymatic hydrolysis of cellulose in aqueous two-phase systems. I. Partition of cellulases from Trichoderma reesei, Biotechnol. Bioeng. 27:1036 (1985)

    Article  PubMed  CAS  Google Scholar 

  6. F. Tjerneld, I. Persson, P.-Å. Albertsson and B. Hahn-Hägerdal, Enzymatic hydrolysis of cellulose in aqueous two-phase systems. II. Semicontinuous conversion of a model substrate, Solka Floc BW200, Biotechnol. Bioeng. 27:1044 (1985)

    Article  PubMed  CAS  Google Scholar 

  7. M.-R. Kula, K.H. Kroner and H. Hustedt, Purification of enzymes by liquid-liquid extraction, Adv. Biochem. Bioeng. 24:73 (1982)

    CAS  Google Scholar 

  8. A. Veide, A.-L. Smeds and S.-O. Enfors, A process for large-scale isolation of ß-galactosidase from E. coli in an aqueous two-phase system, Biotechnol. Bioeng. 25:1789 (1983)

    Article  PubMed  CAS  Google Scholar 

  9. E.T. Hjermstad, Starch hydroxyethyl ethers and other starch ethers, in: “Industrial Gums — Polysaccharides and Their Derivatives,” R.L. Whistler and J.N. BeMiller, eds., Academic Press, New York (1973)

    Google Scholar 

  10. F. Tjerneld, S. Berner, A. Cajarville and G. Johansson, New aqueous two-phase system based on hydroxypropyl starch useful in enzyme purification, Enzyme Microb. Technol. 8:417 (1986)

    Article  CAS  Google Scholar 

  11. Å. Gustafsson, H. Wennerström and F. Tjerneld, Aqueous polymer two-phase systems in biotechnology, Fluid Phase Equilibria 29:365 (1986)

    Article  CAS  Google Scholar 

  12. F. Tjerneld and G. Johansson, U.S. Patent 4,740,304

    Google Scholar 

  13. G. Birkenmeier, G. Kopperschläger, P.-Å. Albertsson, G. Johansson, F. Tjerneld, H.E. Åkerlund, S. Berner and H. Wickström, Fractionation of proteins from human serum by countercurrent distribution, J. Biotechnol. 5:115 (1987)

    Article  CAS  Google Scholar 

  14. F. Tjerneld, G. Johansson and M. Joelsson, Affinity liquid-liquid extraction of lactate dehydrogenase on a large scale, Biotechnol. Bioeng. 30:809 (1987)

    Article  PubMed  CAS  Google Scholar 

  15. S. Lindenfors and I. Jullander, Ethylhydroxyethyl-cellulose, in: “Industrial Gums — Polysaccharides and Their Derivatives,” R.L. Whistler and J.N. BeMiller, eds., Academic Press, New York (1973)

    Google Scholar 

  16. O. Stenberg, Berol Kemi AB, Stenungsund, Sweden, personal communication

    Google Scholar 

  17. I. Persson, F. Tjerneld and B. Hagn-Hägerdal, Cultivation and enzyme production in aqueous two-phase systems with Trichoderma reesei and with Aspergillus phoenicis, Lecture at IVA and EFB Workshop, 10–11 November 1986 in IVA-report 330, Stockholm (1987)

    Google Scholar 

  18. Å. Gustafsson, H. Wennerström and F. Tjerneld, The nature of phase separation in aqueous two-polymer systems, Polymer 27:1768 (1986)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Plenum Press, New York

About this chapter

Cite this chapter

Tjerneld, F. (1989). New Polymers for Aqueous Two-Phase Systems. In: Fisher, D., Sutherland, I.A. (eds) Separations Using Aqueous Phase Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5667-7_69

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-5667-7_69

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5669-1

  • Online ISBN: 978-1-4684-5667-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics