Abstract
The intregrity of cardiac function depends on the energy of the tissue where ATP is produced mainly by oxidative phosphorylation in the mitochondria. Thus, a critical oxygen concentration has been postulated below which function cannot be maintained. Araki et al. (1983) found oxygen consumption fell when cardiac tissue oxygen concentration fell below 10 wmM. However, energy state can be governed by factors other than oxygen concentration (Owen and Wilson, 1974). In the present study, we performed simultaneously measuring the energy state of isolated hearts, by 31P-NMR, and estimating the tissue oxygen concentration by optical spectroscopy. Similar in situ measurements in brain were reported previously (Tamura et al., 1988).
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References
Araki, R., Tamura, M., and Yamazaki, I. 1983. The effect of intracellular oxygen concentration on lactate release, pyridine nucleotide reduction, and respiration rate in the cardiac tissue. Circ. Res., 53:448.
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Owen, C.S., and Wilson, D.F. 1974. Control of respiration by the mitochondrial phosphorylation state. Arch. Biochem. Biophys., 161:581.
Tamura, M., Hazeki, 0., Nioka, S., Chance, B., and Smith, D.S. 1988. The simultaneous measurements of tissue oxygen concentration and energy state by near-infrared and nuclear magnetic resonance spectroscopy. Adv. Exp. Med. Biol., 222:359.
Tamura, M., Oshino, N., Chance, B., and Silver, I. 1978. Optical measurements of intracellular oxygen concentration of rat heart in vitro. Arch. Biochem. Biophys., 191:8.
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© 1989 Plenum Press, New York
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Fukuda, H., Yasuda, H., Shimokawa, S., Tamura, M. (1989). The Oxygen Dependence of the Energy State of Cardiac Tissue: 3 1P-NMR and Optical Measurement of Myoglobin in Perfused Rat Heart. In: Rakusan, K., Biro, G.P., Goldstick, T.K., Turek, Z. (eds) Oxygen Transport to Tissue XI. Advances in Experimental Medicine and Biology, vol 248. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5643-1_63
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DOI: https://doi.org/10.1007/978-1-4684-5643-1_63
Publisher Name: Springer, Boston, MA
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