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Plasmid Vectors Carrying the Replication Origin of Filamentous Single-Stranded Phages

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Genetic Engineering

Part of the book series: Genetic Engineering ((GEPM))

Abstract

Plasmids are convenient vectors for the maintenance of cloned DNA. Inserts are stable, and large amounts of double-stranded (ds) DNA can be purified easily, manipulated in vitro with restriction and ligation enzymes, and the recombinant constructs reintroduced with high efficiency into E. coli by transformation.

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Author information

Authors and Affiliations

  1. European Molecular Biology Laboratory, Postfach 10.2209, 6900, Heidelberg, Federal Republic of Germany

    Gianni Cesareni & James A. H. Murray

Authors
  1. Gianni Cesareni
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  2. James A. H. Murray
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Editor information

Editors and Affiliations

  1. Brookhaven National Laboratory, Upton, New York, USA

    Jane K. Setlow

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© 1987 Plenum Press, New York

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Cesareni, G., Murray, J.A.H. (1987). Plasmid Vectors Carrying the Replication Origin of Filamentous Single-Stranded Phages. In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5377-5_9

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  • DOI: https://doi.org/10.1007/978-1-4684-5377-5_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5379-9

  • Online ISBN: 978-1-4684-5377-5

  • eBook Packages: Springer Book Archive

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