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Aging of Rat Heart Myocytes and Fibroblasts: Relationship between Lipid Composition, Membrane Organization and Biological Properties

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Enzymes of Lipid Metabolism II

Part of the book series: NATO ASI Series ((NSSA,volume 116))

Abstract

The age-related alterations in the lipid composition in two types of cultured rat-heart cells, the myocytes and the fibroblasts, was studied in relation to several biochemical and biophysical parameters. For both cell types aged (14–15 day old) cultures displayed higher mole ratios of sphingomyelin to phosphatidylcholine, as well as elevated cholesterol levels. A concomitant increase was observed in the total protein content of the cells and in the Vmax values of seven marker enzymes. Beating rate of the myocytes was reduced from 160 ± 20 in the young cells to 20 ± 20 in the old myocytes. Fluorescence photobleaching recovery was employed to study the lateral mobility of the lipid probe NBD-phosphatidyl ethanolamine and of membrane glycoproteins that bind succinylated concanavalin A. The mobile fractions of both probes were higher in aged cultures, while the lateral diffusion coefficients were lower. To further demonstrate the dependence of the above parameters on the cellular lipid composition, the lipid composition of old cultures was manipulated by treatments with liposomes (small unilamellar vesicles) of specific compositions. Treatments with liposomes enriched with egg phosphatidylcholine reversed the lipid composition towards that of young (5–6 day old) cultures. This was followed by a concomitant reversal of the measured biochemical and biophysical parameters to the values observed in young cultures. These findings suggest that alterations in the organization and mobility of cell membrane constituents are involved in mediating changes in cellular functions. Since the two cell types demonstrate similar behaviour it appears that the modulation of cellular properties through the membrane lipid composition may be a general phenomenon in many cell types.

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Author information

Authors and Affiliations

  1. Departments of Biochemistry, The Hebrew University - Hadassah Medical School, Jerusalem, 91010, Israel

    Elishalom Yechiel & Yechezkel Barenholz

  2. Departments of Biochemistry, Tel-Aviv University, Tel-Aviv, 69978, Israel

    Yoav I. Henis

Authors
  1. Elishalom Yechiel
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  2. Yoav I. Henis
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  3. Yechezkel Barenholz
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Editor information

Editors and Affiliations

  1. CNRS Center for Neurochemistry and INSERM U44, Louis Pasteur University, Strasbourg, France

    Louis Freysz , Henri Dreyfus  & Raphaël Massarelli ,  & 

  2. Hebrew University-Hadassah School of Medicine, Jerusalem, Israel

    Shimon Gatt

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© 1986 Plenum Press, New York

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Yechiel, E., Henis, Y.I., Barenholz, Y. (1986). Aging of Rat Heart Myocytes and Fibroblasts: Relationship between Lipid Composition, Membrane Organization and Biological Properties. In: Freysz, L., Dreyfus, H., Massarelli, R., Gatt, S. (eds) Enzymes of Lipid Metabolism II. NATO ASI Series, vol 116. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5212-9_67

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  • DOI: https://doi.org/10.1007/978-1-4684-5212-9_67

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5214-3

  • Online ISBN: 978-1-4684-5212-9

  • eBook Packages: Springer Book Archive

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