Abstract
Accurate and rapid measurement of the oxygen concentration in biological samples, particularly when these oxygen concentrations are low, has been the goal of many research workers. By far the most successful method has been the use of oxygen electrodes (for review see Silver, 1984). However it is limited by the stability of the electrode surface and by instabilities in the oxygen diffusion barrier (because it measures the rate of diffusion of oxygen to the cathode). An alternate method, which measures the oxygen dependent quenching of the fluorescence of pyrene butyric acid (Vaughan and Weber, 1970; Knopp and Longmuir, 1972; Opitz and Lübbers, 1984), has been much less useful because the fluorescence intensity is affected by many experimental parameters other than oxygen concentration.
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References
Horie, T. and Vanderkooi, J.M. (1984) Use of phosphorescence at room temperature for the study of biological molecules, Life Chemistry Reports 2, 141–178
Knopp, J.A. and Longmuir, I.S. (1972) Intracellular measurement of oxygen by quenching of fluorescence of pyrene butyric acid, Biochim. Biophys. Acta 279, 393–397.
Opitz, N. and Lubbers, D.W. (1984) Increased resolution power in PO analysis at lower PO„ levels via sensitivity enhanced optical Po sensors (PO optodes) using fluorescence dyes. Adv. in Exptl. Mes. Biot. 180, 2261–267.
Silver, I.A. (1984) Polarographic techniques of oxygen measurements in “Oxygen: An in depth study of its pathophysiology” (S.F. Gottlieb, I.S. Longmuir and J.R. Totter, eds.) Undersea Med. Soc. pub No. 62(ws) 3–1–84 p. 215–238.
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© 1986 Plenum Press, New York
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Vanderkooi, J.M., Wilson, D.F. (1986). A New Method for Measuring Oxygen Concentration in Biological Systems. In: Longmuir, I.S. (eds) Oxygen Transport to Tissue VIII. Advances in Experimental Medicine and Biology, vol 200. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5188-7_25
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DOI: https://doi.org/10.1007/978-1-4684-5188-7_25
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