Abstract
The simplest way to study membrane current fluctuations from ionic channels would be to place the channels into a steady, partly activated state and to monitor the fluctuations in the current around its steady mean value. In practice, an experiment of this kind is often not possible because of properties of the channels themselves. Acetylcholine-receptor channels, for example, show a desensitization process in which the agonist-induced membrane current declines with time. Some voltage-activated channels show a similar kind of behavior: during a steady depolarization, Na+ channels spontaneously inactivate. These changes in membrane current with time pose both practical and theoretical problems for noise analysis. Since the size of the fluctuations is typically only a few percent of the mean current, a serious practical problem arises whenever a small baseline drift occurs, since drift can seriously contaminate a power spectrum computed from the fluctuations (Fig. 1). In many experimental situations the baseline drift can be estimated and removed from the records; however a theoretical problem still remains: how should the fluctuations be interpreted when the mean current itself changes with time?
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References
Conti, F., Neumcke, B., Normer, W., and Stampfli, R., 1980, Conductance fluctuations from the inactivation process of sodium channels in myelinated nerve fibres, J. Physiol., 308:277.
Fenwick, E. M., Marty A., and Neher, E., 1982, Sodium and calcium channels in bovine chromaffin cells, J. Physiol., 331:599.
Hagiwara, S., and Ohmori, H., 1982, Studies of calcium channels in rat clonal pituitary cells with patch electrode voltage clamp, J. Physiol., 331:231.
Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J., 1981, Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches, PflĂĽgers Arch., 391:85.
Neumcke, B., and Stämpfli, R., 1982, Sodium currents and sodium-current fluctuations in rat myelinated nerve fibres, J. Physiol., 329:163.
Neumcke, B., and Stämpfli, R., 1983, Alteration of the conductance of Na+ channels in the nodal membrane of frog nerve by holding potential and tetrodotoxin. Biochim. Biophys. Acta, 727:177.
Ohmori, H., 1981, Unitary current through sodium channel and anomalous rectifier channel estimated from transient current in the tunicate egg, J. Physiol, 311:289.
Schwartz, E. A., 1975, Rod-rod interaction in the retina of the turtle, J. Physiol., 246:617.
Sigworth, F. J., 1980a, The variance of sodium current fluctuations at the node of Ranvier, J. Physiol., 307:97.
Sigworth, F. J., 1980b, The conductance of sodium channels under conditions of reduced current at the node of Panvier, J. Physiol., 307:131.
Sigworth, F.J., 1981a, Covariance of nonstationary sodium current fluctuations in the node of Ranvier, Biophys. J., 34:111.
Sigworth, F.J., 1981b, Interpreting power spectra from nonstationary membrane current fluctuations, Biophys. J., 35:289.
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© 1984 Plenum Press, New York
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Sigworth, F.J. (1984). Nonstationary Noise Analysis of Membrane Currents. In: Eisenberg, R.S., Frank, M., Stevens, C.F. (eds) Membranes, Channels, and Noise. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4850-4_2
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DOI: https://doi.org/10.1007/978-1-4684-4850-4_2
Publisher Name: Springer, Boston, MA
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