Skip to main content
SpringerLink
Log in

Guinea Pig Alveolar Macrophages Probably Kill M. Tuberculosis H37Rv and H37Ra In Vivo by Producing Hydrogen Peroxide

  • Conference paper
Host Defenses to Intracellular Pathogens

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 162))

Abstract

The major phagocytic cell involved in host resistance to Mycobacterium tuberculosis infection is probably the alveolar macrophage. It seems likely that macrophages, at least in the immune animal, can kill M. tuberculosis (1) and this might involve the production of hydrogen peroxide (H2O2) by the macrophages. Macrophages release H2O2 in vitro when exposed to phagocytic stimuli or certain soluble agents that perturb the plasma membrane (8, 9). H2O2 can kill M. tuberculosis and resistance to H2O2 in vitro correlates with high virulence in the guinea pig (11, 5).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Greening, A.P., Rees, A.D.M. and Lowrie, D.B. (1980). Human alveolar macrophage staphylocidal function. Clin. Sci. 59, 14.

    Google Scholar 

  2. Guilbault, G.G., Brignac, P.J. and Juneau, M. (1968). New substrates for fluorimetric determination of oxidative enzymes. Anal. Chem. 40, 1256–1263.

    Article  PubMed  CAS  Google Scholar 

  3. Harmsen, A.G. and Jeska, E.L. (1980). Surface receptors on porcine alveolar macrophages and their role in phagocytosis. J. Reticuloendoth. Soc. 27, 631–637.

    CAS  Google Scholar 

  4. Hunninghake, G.W. and Fauci, A.S. (1976). Immunological reactivity of the lung. 1. A guinea-pig model for the study of pulmonary mononuclear cell subpopulations. Cell. Immunol. 26, 89–97.

    Article  PubMed  CAS  Google Scholar 

  5. Jackett, P.S., Aber, V.R. and Lowrie, D.B. (1978). Virulence and resistance to superoxide, low pH and hydrogen peroxide among strains of Mycobacterium tuberculosis. J. Gen. Microbiol, 104, 37–45.

    PubMed  CAS  Google Scholar 

  6. Jackett, P.S., Aber, V.R., Mitchison, D.A. and Lowrie, D.B. (1981). The contribution of hydrogen peroxide resistance to virulence of Mycobacterium tuberculosis during the first six days after intravenous infection of normal and BCG-vaccinated guinea-pigs. Br. J. Exp. Path. 62, 34–40.

    CAS  Google Scholar 

  7. Johnston, R.B., Godzik, C.A. and Cohn, Z.A. (1978). Increased superoxide anion production by immunologically activated and chemically elicited macrophages. J. Exp. Med., 148, 115–127.

    Article  PubMed  CAS  Google Scholar 

  8. Kaneda, M., Kakinum, K., Yamaguchi, T. and Shimada, K. (1980). Comparative studies on alveolar macrophages and polymorphonuclear leukocytes. II. The ability of guinea-pig alveolar macrophages to produce H2O2. J. Biochem., 88, 1159–1165.

    PubMed  CAS  Google Scholar 

  9. Klebanoff, S.J. and Hamon, C.B. (1975). Antimicrobial systems of mononuclear phagocytes. In Mononuclear phagocytes in immunity, infection and pathology. pp. 507–529. Edited by R. van Furth. Oxford: Blackwell Scientific.

    Google Scholar 

  10. Lurie, M.B. (1964). Resistance to tuberculosis; experimental studies in native and acquired defensive mechanisms. Cambridge, Mass: Harvard University Press.

    Google Scholar 

  11. Mitchison, D.A., Selkon, J.B. and Lloyd, J. (1963). Virulence in the guinea-pig, susceptibility to hydrogen peroxide, and catalase activity of isoniazid-sensitive tubercle bacilli from South Indian and British patients. J. Path. Bact. 86, 377–386.

    Article  PubMed  CAS  Google Scholar 

  12. Montarroso, A.M. and Myrvik, Q.N. (1978). The effect of BCG vaccination on the IgG and complement receptors on rabbit alveolar macrophages. J. Reticuloendoth. Soc., 24, 93–99.

    CAS  Google Scholar 

  13. Nathan, C.F., Nogueira, N., Juangbhanich, C.W., Ellis, J. and Cohn, Z.A. (1979). Activation of macrophages in vivo and in vitro. Correlation between hydrogen peroxide release and killing of Trypanosoma cruzi. J. Exp. Med., 149, 1056–1068.

    Article  PubMed  CAS  Google Scholar 

  14. Wolff, L.J., Boxer, L.A., Allen, J.M. and Baehner, R.L. (1978). The selective effect of hypoxia on the guinea-pig alveolar macrophage membrane. J. Reticuloendoth. Soc., 24, 377–382.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. MRC Unit for Laboratory Studies of Tuberculosis, Royal Postgraduate Medical School, London, W12 OHS, England

    P. S. Jackett, P. W. Andrew, V. R. Aber & D. B. Lowrie

Authors
  1. P. S. Jackett
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. W. Andrew
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. R. Aber
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. B. Lowrie
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Temple University School of Medicine, Philadelphia, Pennsylvania, USA

    Toby K. Eisenstein

  2. Smith, Kline, and French Laboratories, Philadelphia, Pennsylvania, USA

    Paul Actor

  3. College of Medicine, University of South Florida, Tampa, Florida, USA

    Herman Friedman

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Plenum Press, New York

About this paper

Cite this paper

Jackett, P.S., Andrew, P.W., Aber, V.R., Lowrie, D.B. (1983). Guinea Pig Alveolar Macrophages Probably Kill M. Tuberculosis H37Rv and H37Ra In Vivo by Producing Hydrogen Peroxide. In: Eisenstein, T.K., Actor, P., Friedman, H. (eds) Host Defenses to Intracellular Pathogens. Advances in Experimental Medicine and Biology, vol 162. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4481-0_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-4481-0_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4483-4

  • Online ISBN: 978-1-4684-4481-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation