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Amniotic Fluid Cell Culture

  • Chapter
Genetic Disorders and the Fetus

Abstract

Cellular pleomorphism is a characteristic feature of amniotic fluid examined microscopically immediately following amniocentesis. Multiple cell types (with differing sites of origin) adhere to glass or culture vessels within 12-24 hr of plating (see Chapter 9 for more details). In amniotic fluid obtained in the late third trimester, many authors have noticed the presence of cells that stain orange with Nile blue sulfate (Brosens and Gordon, 1965; Anderson and Griffiths, 1968; Brosens et al., 1969). The four types of amniotic fluid cells observed after Nile blue sulfate cell staining are the parabasal, the blue intermediate, the anucleated blue squamous, and the anucleated orange staining (Husain and Sinclair, 1971). The appearance of these stained cells seems to correlate with the earliest keratinization of the stratum corneum, starting at about 17 weeks’ gestation (Serri and Montagna, 1961; Parmley and Miller, 1969). It is just possible that this evidence of keratinization prior to 20 weeks of pregnancy may ultimately lead to the prenatal detection of hereditary disorders of keratin, such as the ichthyoses (Goodman, 1970).

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References

  • Abbo, G., and Zellweger, H., 1970, Prenatal determination of fetal sex and chromosomal complement, Lancet 1:216.

    PubMed  CAS  Google Scholar 

  • Allison, A. C., and Paton, G. R., 1966, Chromosomal abnormalities in human diploid cells infected with mycoplasma and their possible relevance to the etiology of Down’s syndrome (mongolism), Lancet 2:1229.

    PubMed  CAS  Google Scholar 

  • Anderson, A. B., and Griffiths, A. D., 1968, Estimation of duration of gestation by amniotic fluid cytology, J. Obstet. Gynaecol. Br. Commonw. 75:300.

    PubMed  CAS  Google Scholar 

  • Austin, C. R., 1962, Sex chromatin in embryonic and fetal tissue, Acta. Cytol. 6:61.

    PubMed  CAS  Google Scholar 

  • Barile, M. F., and Kern, J., 1971, Isolation of mycoplasma arginini from commercial bovine sera and its implication in contaminated cell cultures, Proc. Soc. Exp. Biol. Med. 138:432.

    PubMed  CAS  Google Scholar 

  • Bradbury, J., and Jordan, F., 1972, Studies on the adsorption of certain medium proteins to M. gallisepticum and their influence on agglutination and haemagglutination, J. Hyg. 70:267.

    CAS  Google Scholar 

  • Brosens, I., and Gordon, H., 1965, The cytological diagnosis of ruptured membranes using nile blue sulphate staining, J. Obstet. Gynaecol. Br. Commonw. 72:342.

    PubMed  CAS  Google Scholar 

  • Brosens, I. A., Gordon, H., and Baert, A., 1969, Prediction of fetal maturity with combined cytological and radiological methods, J. Obstet. Gynaecol. Br. Commonw. 76:20.

    PubMed  CAS  Google Scholar 

  • Ceccarini, C., and Eagle, H., 1971, pH as a determinant of cellular growth and contact inhibition, Proc. Natl. Acad. Sci. USA 68:229.

    PubMed  CAS  Google Scholar 

  • Cederqvist, L. L., Wennerstrom, C., Senterfit, L. B., et al., 1973, Simplified method for the accelerated growth of amniotic fluid cell cultures. Am. J. Obstet. Gynecol. 116:871.

    PubMed  CAS  Google Scholar 

  • Chang, R. S., 1968, Observations on the growth phases of human amnion cell cultures, J. Natl. Cancer Inst. 40:491.

    PubMed  CAS  Google Scholar 

  • Chitham, R. G., Quayle, S. J., and Hill, L., 1973, The selection of a method for the culture of cells from amniotic fluid, 7. Clin. Path. 26:721.

    CAS  Google Scholar 

  • Cohen, S., and Carpenter, G., 1975, Human epidermal growth factor: Isolation and chemical biological properties, Proc. Natl. Acad. Sci. USA 72:1317.

    PubMed  CAS  Google Scholar 

  • Conover, J. H., and Hirschhorn, K., 1971, Establishment and genetic stability of human amnion cell Hnes, Lab. Invest. 24:38.

    PubMed  CAS  Google Scholar 

  • Court-Brown, W. M., Harnden, D. C., Jacobs, P., et al., 1964, Abnormalities of the sex chromosomes complement in man, Med. Res. Counc. Spec. Rep. Ser. (London) 305:1.

    CAS  Google Scholar 

  • Cox, D. M., Niewczas-Late, V., Riffell, M. I., et al., 1974, Chromosomal mosaicism in diagnostic amniotic fluid cells, Pediatr. Res. 8:679.

    PubMed  CAS  Google Scholar 

  • Dancis, J., 1968, The antepartum diagnosis of genetic diseases, J. Pediatr. 72:301.

    PubMed  CAS  Google Scholar 

  • Dioguardi, N., Agostani, A., Fiorelli, G., et al., 1963, Characterization of lactic dehydrogenase of normal human granulocytes. J. Lab. Clin. Med. 61:713.

    PubMed  CAS  Google Scholar 

  • Doherty, R. A., Felix, J. S., and Ridge, S. C., 1974, Evaluation of a new round glass coverslip for cytological examination of cultured amniotic fluid cells, Humangenetik 25:217.

    PubMed  CAS  Google Scholar 

  • Eagle, H., 1971, Buffer combinations for mammalian cell culture. Science 174:500.

    PubMed  CAS  Google Scholar 

  • Earley, E. M., Finley, S. C., Finley, W. H., et al., 1976, Karyology of primary human fetal cell cultures. In Vitro 12:639.

    PubMed  CAS  Google Scholar 

  • Felix, J. S., Doherty, R. A., Davis, H. T., et al., 1974, Amniotic fluid cell culture. I. Experimental design for evaluating cell culture variables: Determination of optimal fetal calf serum concentration, Pediatr. Res. 8:870.

    PubMed  CAS  Google Scholar 

  • Ferguson-Smith, M. E., Ferguson-Smith, M. A., Nevin, N. C., et al., 1971, Chromosome analysis before birth and its value in genetic counselling, Br. Med. J. 4:69.

    PubMed  CAS  Google Scholar 

  • Fogh, J., and Fogh, H., 1964, Chromosome changes in Fl human amnion cells after PPLO infection, Mammalian cytology and somatic cell genetics conference, San Juan, Puerto Rico.

    Google Scholar 

  • Fogh, J., and Fogh, H., 1965, Chromosome changes in PPLO-infected Fl human amnion cells, Proc. Soc. Exp. Biol. Med. 119:233.

    PubMed  CAS  Google Scholar 

  • Fogh, J., and Fogh, H., 1973, Chromosome changes in cell culture induced by mycoplasma infection, Acta.N.Y. Acad. Sci. 225:311.

    Google Scholar 

  • Freeman, A. E., Igel, H. J., Herrman, B. J., et al., 1976, Growth and characterization of human skin epithelial cell cultures. In Vitro 12:352.

    PubMed  CAS  Google Scholar 

  • Gadow, E. C., Paz, J. E., Castilla, E. E., et al., 1976, Prenatal detection of chromosome aberrations after intercontinental transport of amniotic fluid. Int. J. Gynaecol. Obstet. 14:164.

    PubMed  CAS  Google Scholar 

  • Garver, K. L., Marchese, S. L., Boas, E. G., et al., 1976, Amniotic-fluid-culture failure: Possible role of syringes, N. Engl. J. Med. 295:286.

    PubMed  CAS  Google Scholar 

  • Golbus, M. S., Conte, F. A., Schneider, E. L., et al., 1974, Intrauterine diagnosis of genetic defects: Resuhs, problems and follow-up of one hundred cases in a prenatal genetic detection center. Am.J. Obstet. Gynecol. 118:897.

    Google Scholar 

  • Goodman, R. M., 1970, Genetic Disorders of Man, Little, Brown, Boston.

    Google Scholar 

  • Gospodarowicz, D., and Moran, J. S., 1975, Mitogenic effect of fibroblast growth factor on early passage cultures of human and murine fibroblasts, J. Cell Biol. 66:451.

    PubMed  CAS  Google Scholar 

  • Gospodarowicz, D., Moran, J. S., and Owashi, N. D., 1977, Effects of fibroblast growth factor and epidermal growth factor on the rate of growth of amniotic fluid-derived cells, J. Clin. Endocrinol. Metab. 44:651.

    PubMed  CAS  Google Scholar 

  • Gray, C., Davidson, R. G., and Cohen, M. M., 1971, A simplified technique for the culture of amniotic fluid cells, J. Pediatr. 79:199.

    Google Scholar 

  • Greene, A. E., Toji, L., Nichols, W. W., et al., 1973, Amniocentesis cells: Culture, cryogenic storage, isozymes, and finite life in vitro. In Vitro 9:156.

    PubMed  CAS  Google Scholar 

  • Gregson, N. M., 1970, A technique for culturing cells from amniotic fluid. Lancet 1:84.

    PubMed  CAS  Google Scholar 

  • Hahneman, N., 1972, Possibility of culturing foetal cells at early stages of pregnancy, Clin. Genet. 3:286.

    Google Scholar 

  • Hasholt, L., 1976, Behaviour of cell cultures from human amniotic fluid, J. Med. Genet. 13:34.

    PubMed  CAS  Google Scholar 

  • Hayflick, L., 1961, The establishment of a Hne (WISH) of human amnion cells in continuous cultivation, Exp. Cell Res. 23:14.

    PubMed  CAS  Google Scholar 

  • Hayflick, L., and Stanbridge, E., 1967, Isolation and identification of mycoplasma from human clinical materials, N.Y. Acad. Sci. 143:608.

    CAS  Google Scholar 

  • Hoehn, H., Bryant, E. M., Karp, L. E., et al., 1974, Cultivated cells from diagnostic amniocentesis in second trimester pregnancies. I. Clonal morphology and growth potential, Pediatr. Res. 8:746.

    PubMed  CAS  Google Scholar 

  • Hoehn, H., Bryant, E. M., Karp, L. E., et al., 1975a, Cultivated cells from diagnostic amniocentesis in second trimester pregnancies. II. Cytogenetic parameters as functions of clonal type and preparative technique, Clin. Genet. 7:29.

    PubMed  CAS  Google Scholar 

  • Hoehn, H., Bryant, E. M., Fantel, A. G., et al., 1975b, Cultivated cells from diagnostic amniocentesis in second trimester pregnancies. III. The fetal urine as a potential source of clonable cells, Humangenetik 29:285.

    PubMed  CAS  Google Scholar 

  • Hoyes, A. D., 1968, Ultrastructure of the cells of the amniotic fluid, J. Obstet. Gynaecol. Br. Commonw. 75:949.

    PubMed  CAS  Google Scholar 

  • Hudson, E. A., 1975, The cytological difference between amniotic fluids of male and female fetuses, Br. J. Obstet. Gynaecol. 82:523.

    PubMed  CAS  Google Scholar 

  • Husain, O. A., and Sinclair, L., 1971, Estimation of maturity in the foetus and newborn: Studies on the cytology of amniotic fluid and of the newborn infant’s skin in relation to maturity of the infant, Proc. R. Soc. Med. 64:1213.

    PubMed  CAS  Google Scholar 

  • Jacobson, B., and Barter, R. H., 1967, Intrauterine diagnosis and management of genetic defects. Am. J. Obstet. Gynecol. 99:796.

    PubMed  CAS  Google Scholar 

  • Jezequel, A. M., Shreeve, M. M., and Steiner, J. W., 1967, Segregation of nucleolar components in mycoplasma-infected cells. Lab. Invest. 16:287.

    PubMed  CAS  Google Scholar 

  • Ju, K. S., Park, I. J., and Jones, H. W., Jr., 1976, The culturability of fibroblasts from the skin of abortuses after intra-amniotic instillation of urea or prostaglandin, J. Obstet. Gynecol. 125:1155.

    CAS  Google Scholar 

  • Kerenyi, T. D., and Walker, B., 1977, The preventability of bloody taps in second trimester amniocentesis by ultrasound scanning. Obstet. Gynecol. 50:61.

    PubMed  CAS  Google Scholar 

  • Knorr-Gartner, H., and Harle, I., 1972, A modified method of culturing human amniotic fluid cells for prenatal detection of genetic disorders, Humangenetik 14:333.

    PubMed  CAS  Google Scholar 

  • Langreder, W., 1952, Zur Zytologie des Fruchtwassers (Blasensprungdiagnose und Geschlechtsvoraussage), Z. Geburtshilfe Gynaekol. 136:136.

    CAS  Google Scholar 

  • Lee, C. L. Y., Gregson, N. M., and Walker, S., 1970, Eliminating red blood-cells from amnioticfluid samples. Lancet 2:316.

    PubMed  CAS  Google Scholar 

  • Levine, E. M., 1972, Mycoplasma contamination of animal cell cultures: A simple rapid detection method, Exp. Cell Res. 74:99.

    PubMed  CAS  Google Scholar 

  • Lie, S. O., McKusick, V. A., and Neufeld, E. F., 1972, Simulation of genetic mucopolysaccharidoses in normal human fibroblasts by alteration of pH of the medium, Proc. Natl. Acad. Sci. USA 69:2361.

    PubMed  CAS  Google Scholar 

  • Lie, S. O., Schofield, B. H., Taylor, H. A., Jr., et al., 1973, Structure and function of the lysosomes of human fibroblasts in culture: Dependence on medium pH., Pediatr. Res. 7:13.

    PubMed  CAS  Google Scholar 

  • Macintyre, M. N., 1971, Chromosomal problems of intrauterine diagnosis, in: Intrauterine Diagnosis, Birth Defects Orig. Art. Ser. 3(5): 10.

    Google Scholar 

  • McKeehan, W. L., McKeehan, K. A., Hammond, S. L., et al., 1977, Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein, In Vitro 13:399.

    PubMed  CAS  Google Scholar 

  • Milunsky, A., 1973, The Prenatal Diagnosis of Hereditary Disorders, Thomas, Springfield, Ill.

    Google Scholar 

  • Milunsky, A., 1975, The Prevention of Genetic Disease and Mental Retardation, Saunders, Philadelphia.

    Google Scholar 

  • Milunsky, A., and Atkins, L., 1974, Prenatal diagnosis of genetic disorders: An analysis of experience with 600 cases, J. Am. Med. Assoc. 230:232.

    CAS  Google Scholar 

  • Milunsky, A., and Bender, C. S., 1979, Failure of amniotic fluid cell growth with toxic tubes. New Engl. J. Med. 301:47.

    PubMed  CAS  Google Scholar 

  • Milunsky, A., Atkins, L., and Littlefield, J. W., 1972, Amniocentesis for prenatal genetic studies. Obstet. Gynecol. 40:104.

    PubMed  CAS  Google Scholar 

  • Melancon, S. B., Lee, S. Y., and Nadler, J. L., 1971, Histidase activity in cultivated human amniotic fluid cells. Science 173:627.

    PubMed  CAS  Google Scholar 

  • Mulcahy, M. T., and Jenkyn, J., 1976, Maternal cell contamination: A problem in amniocentesis. Hum. Genet. 34:115.

    PubMed  CAS  Google Scholar 

  • Nadler, H. L., 1968, Antenatal detection of hereditary disorders. Pediatrics 42:912.

    PubMed  CAS  Google Scholar 

  • Nadler, H. L., 1969, Prenatal detection of genetic defects, J. Pediatr. 74:132.

    PubMed  CAS  Google Scholar 

  • Nadler, H. L., and Gerbie, A., 1971, Present status of amniocentesis in intrauterine diagnosis of genetic defects. Obstet. Gynecol. 38:789.

    PubMed  CAS  Google Scholar 

  • Nadler, H. L., and Ryan, C. A., 1974, Amniotic cell culture, in: Human Chromosome Methodology, 2nd ed. (J. J. Yunis, ed.), p. 185, Academic Press, New York.

    Google Scholar 

  • Nelson, M. M., and Emery, A. E. H., 1970, Amniotic fluid cells, prenatal sex prediction and culture, Br. Med. J. 1:523.

    PubMed  CAS  Google Scholar 

  • Nelson, M. M., and Emery, A. E. H., 1973, Amniotic fluid cell cultures, J. Med. Genet. 10:19.

    PubMed  CAS  Google Scholar 

  • Nelson, L. H., Goodman, H. O., and Brown, S. H., 1977, Ultrasonography preceding diagnostic amniocentesis and its effect on amniotic fluid cell growth. Obstet. Gynecol. 50:65.

    PubMed  CAS  Google Scholar 

  • Nieland, M. L., Parmley, T. H., and Woodruff, J. D., 1970, Ultrastructural observations on amniotic fluid cells. Am. J. Obstet. Gynecol. 108:1030.

    PubMed  CAS  Google Scholar 

  • Nielsen, J., 1976, Cell selection in vivo in normal/aneuploid chromosome abnormahties, Hum. Genet. 32:203.

    PubMed  CAS  Google Scholar 

  • Niermeijer, M. F., Halley, D., Sachs, E., et al., 1973, Transport and storage of amniotic fluid samples for prenatal diagnosis of metabolic diseases, Humangenetik 20:175.

    PubMed  CAS  Google Scholar 

  • Nigra, T. P., Martin, G. R., and Eagle, H., 1973, The effect of environmental pH on collagen synthesis by cultured cells, Biochem. Biophys. Res. Commun. 53:272.

    PubMed  CAS  Google Scholar 

  • Packer, L., and Smith, J. R., 1974, Extension of the lifespan of cultured normal human diploid cells by vitamin E, Proc. Natl. Acad. Sci. USA 71:4763.

    PubMed  CAS  Google Scholar 

  • Packer, L., and Smith, J. R., 1977, Extension of the lifespan of cultured normal human diploid cells by vitamin E: A reevaluation, Proc. Natl. Acad. Sci. USA 74:1640.

    PubMed  CAS  Google Scholar 

  • Park, I. J., Wentz, A. C., and Jones, W. H., Jr., 1973, The viability of fetal skin of abortuses induced by saline or prostaglandin. Am. J. Obstet. Gynecol. 115:274.

    PubMed  CAS  Google Scholar 

  • Parmley, T. H., and Miller, E., 1969, Fetal maturity and amniotic fluid analysis. Am. J. Obstet. Gynecol. 105:354.

    PubMed  CAS  Google Scholar 

  • Parshad, R., and Sanford, K. K., 1977, Intermittent exposure to fluorescent light extends lifespan of human diploid fibroblasts in culture. Nature (London) 268:736.

    CAS  Google Scholar 

  • Paton, G. R., Jacobs, J. P., and Perkins, F. T., 1965, Chromosome changes in human diploid-cell cultures infected with mycoplasma. Nature (London) 207:43.

    CAS  Google Scholar 

  • Purvis-Smith, S. G., Jones, M. A., and Wertelecki, W., 1978, Amniotic-fluid cell-culture failure and syringe toxicity, Lancet 1:993.

    PubMed  CAS  Google Scholar 

  • Rheinwald, J. G., and Green, 1977, Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature (London) 265:421.

    CAS  Google Scholar 

  • Robinson, A., Bowes, W., Droegemueller, W., et al., 1973, Intrauterine diagnosis: Potential complications. Am. J. Obstet. Gynecol. 117:937.

    Google Scholar 

  • Robinson, L. B., Wichelbrausen, R. H., and Razinan, B., 1956, Contamination of human cell cultures by pleuropneumonia organisms. Science 124:1147.

    PubMed  CAS  Google Scholar 

  • Rüdiger, H. W., Wolff, R., Wendel, U., et al., 1974, Enhancement of amniotic fluid cell growth in culture, Humangenetik 22:81.

    PubMed  Google Scholar 

  • Schmid, W., 1975, A technique for in situ karyotyping of primary amniotic fluid cell cultures, Humangenetik 30:325.

    PubMed  CAS  Google Scholar 

  • Schneider, E. L., and Stanbridge, E. J., 1975, Comparison of methods for the detection of mycoplasmal contamination of cell cultures: A review, In Vitro 11:20.

    PubMed  CAS  Google Scholar 

  • Schneider, E. L., Stanbridge, E. J., Epstein, C. J., et al., 1974, Mycoplasma contamination of cultured amniotic fluid cells, a potential hazard to prenatal chromosomal diagnosis. Science 184:477.

    PubMed  CAS  Google Scholar 

  • Serri, P., and Montagna, W., 1961, The structure and function of the epidermis, Pediatr. Clin. N. Am. 8:917.

    Google Scholar 

  • Simpson, N. E., Dallaire, L., Miller, J. R., et al., 1976, Prenatal diagnosis of genetic disease in Canada: Report of a collaborative study, Can. Med. Assoc. J. 115:739.

    PubMed  CAS  Google Scholar 

  • Sonnenschein, C., and Chang, R. S., 1969, Karyotype analysis of normal human amnion cells in the recovery phase, Exp. Cell Res. 57:328.

    Google Scholar 

  • Stanbridge, E., 1971, Mycoplasmas and cell cultures, Bacteriol. Rev. 35:206.

    PubMed  CAS  Google Scholar 

  • Steele, M. W., and Breg, W. R., Jr., 1966, Chromosome analysis of human amniotic fluid cells. Lancet 1:383.

    PubMed  CAS  Google Scholar 

  • Sutherland, G. R., and Bain, A. D., 1973, Antenatal diagnosis of inborn errors of metabolism: Tissue culture aspects. Hum. Genet. 20:251.

    CAS  Google Scholar 

  • Sutherland, G. R., Bauld, R., and Bain, D. J., 1974, Observations on human amniotic fluid cell strains in serial culture, J. Med. Genet. 11:190.

    PubMed  CAS  Google Scholar 

  • Takaoka, T., and Katsuta, H., 1975, An improved synthetic medium suitable for tissue culture of various mammalian cells, jpn J. Exp. Med. 45:11.

    CAS  Google Scholar 

  • Taylor, A. I., 1968, Cell selection in vivo in normal/G trisomic mosaics. Nature (London) 219:1028.

    CAS  Google Scholar 

  • Taylor, A. E., 1970, Further observations of cell selection in vivo in normal/G trisomic mosaics. Nature (London) 227:163.

    CAS  Google Scholar 

  • Therkelsen, A., 1976, Cultures of cells from amniotic fluid, in:Prenatal Diagnosis (A. Boue, ed.), p. 55, INSERM U.73, Paris.

    Google Scholar 

  • Timson, J., and Price, D. J., 1970, Use of thymine to increase yield of mitoses in lymphocyte cultures. Lancet 1:1397.

    PubMed  CAS  Google Scholar 

  • Todaro, G. J., Aaronson, S. A., and Rands, E., 1971, Rapid detection of mycoplasma-infected cell cultures, Exp. Cell Res. 65:256.

    PubMed  CAS  Google Scholar 

  • Valenti, C., and Kehaty, T., 1969, Culture of cells obtained by amniocentesis, 7. Lab. Clin. Med. 73:355.

    CAS  Google Scholar 

  • Valenti, C., Lin, C. C., Baum, A., et al., 1972, Prenatal sex determination, Am. J. Obstet. Gynecol. 112:890.

    PubMed  CAS  Google Scholar 

  • Valenti, C., Schutta, E. F., and Kehaty, T., 1969, Cytogenetic diagnosis of Down’s syndrome in utero, J.Am. Med. Assoc. 207:1513.

    CAS  Google Scholar 

  • Van Leeuwen, L., Jacoby, H., and Charles, D., 1965, Exfoliative cytology of amniotic fluid, Acta Cytol. 9:442.

    Google Scholar 

  • Votta, R. A., Gagneten, C. B., Parada, O., et al., 1968, Cytologic study of amniotic fluid in pregnancy. Am. J. Obstet. Gynecol. 102:571.

    PubMed  CAS  Google Scholar 

  • Wachtel, E., Gordon, H., and Olsen, E., 1969, Cytology of amniotic fluid, J. Obstet. Gynaecol. Br. Commonw. 76:596.

    PubMed  CAS  Google Scholar 

  • Wahlstrom, J., Brosset, A., and Bartsch, F., 1970, Viability of amniotic cells at different stages of gestation. Lancet 2:1037.

    PubMed  CAS  Google Scholar 

  • Wang, R., 1975, Lethal effect of “daylight” fluorescent light on human cells in tissue culture medium, Photochem. Photobiol. 21:373.

    PubMed  CAS  Google Scholar 

  • Wang, R. J., 1976, Effect of room fluorescent light on the deterioration of tissue culture medium. In Vitro 12:19.

    PubMed  CAS  Google Scholar 

  • Wang, R., Stoien, J., and Landa, F., 1974, Lethal effect of near-ultraviolet irradiation on mammalian cells in culture. Nature (London) 247:43.

    CAS  Google Scholar 

  • Wolf, U., 1974, Culture and preparation of cells from amniotic fluid, in: Methods in Human Cytogenetics (H. G. Schwarzacher and U. Wölk, eds.), Springer-Verlag, New York.

    Google Scholar 

  • Wood, S., 1975, The effect of environmental pH upon acid hydrolase activities of cultured human diploid fibroblasts, Exp. Cell Res. 96:317.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pediatrics, Harvard Medical School, 02115, Boston, Massachusetts, USA

    Aubrey Milunsky

  2. Genetics Division, Eunice Kennedy Shriver Center, 02154, Waltham, Massachusetts, USA

    Aubrey Milunsky

  3. Children’s Service, Massachusetts General Hospital, 02114, Boston, Massachusetts, USA

    Aubrey Milunsky

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  1. Department of Pediatrics, Harvard Medical School, 02115, Boston, Massachusetts, USA

    Aubrey Milunsky MB.B.Ch., M.R.C.P., D.C.H. (Assistant Professor, Director, Medical Geneticist) (Assistant Professor, Director, Medical Geneticist)

  2. Genetics Division, Eunice Kennedy Shriver Center, 02154, Waltham, Massachusetts, USA

    Aubrey Milunsky MB.B.Ch., M.R.C.P., D.C.H. (Assistant Professor, Director, Medical Geneticist) (Assistant Professor, Director, Medical Geneticist)

  3. Children’s Service, Massachusetts General Hospital, 02114, Boston, Massachusetts, USA

    Aubrey Milunsky MB.B.Ch., M.R.C.P., D.C.H. (Assistant Professor, Director, Medical Geneticist) (Assistant Professor, Director, Medical Geneticist)

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Milunsky, A. (1979). Amniotic Fluid Cell Culture. In: Milunsky, A. (eds) Genetic Disorders and the Fetus. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3438-5_4

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  • DOI: https://doi.org/10.1007/978-1-4684-3438-5_4

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