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Signs of Hypoxia in the Small Intestine of the Rat during Hemorrhagic Shock

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Oxygen Transport to Tissue

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 37 A))

Abstract

The photometric measurement of fluorescence emitted by reduced pyridine nucleotides upon ultraviolet stimulation (1,3) and the polarographic measurement of PO2 (5) in tissues and organs have provided detailed information about the local effects of disturbances of O2 supply and of microcirculation. Under experimental conditions in which circulating blood is a factor it is also necessary to measure absorbancy changes of hemoglobin by reflectance photometry in order to accurately evaluate changes in reduced PN fluorescence (6,9,11). In the present study both the photometric and polarographic techniques have been focused on the small intestine of urethane anesthetized rats subjected to periodic, standardized hemorrhages.

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References

  1. Chance, B., Cohen, P., Jöbsis, F., Schoener, B.: Intracellular oxidation-reduction states in vivo. Science 137, 499–508 (1962).

    Article  PubMed  CAS  Google Scholar 

  2. Chance, B., Oshino, N., Sugano, T.: Cytochrome c and NADH as indicators of electron flow and energy coupling in mitochondria. International symposium on oxygen transport to tissue. April, 1973.

    Google Scholar 

  3. Chance, B., Schoener, B., Schindler, F.: The intracellular oxidation-reduction state. In: Oxygen in the animal organism, ed. by R. Dickens and E. Neil. New York, Pergamon 1963, pp. 367–388.

    Google Scholar 

  4. Kessler, M.: Normale und kritische Sauerstoffversorgung der Leber bei Normo-und Hypothermie. Habil.-Schrift, Marburg 1968.

    Google Scholar 

  5. Kessler, M., Bruley, D.F., Clark, L.C., Lubbers, D.W., Silver, I.A., Strauss, J.: Oxygen supply, theoretical and practical aspects of oxygen supply and microcirculation of tissue. Urban and Schwarzenberg, München-Berlin 1973.

    Google Scholar 

  6. Kobayashi, S., Nishiki, K., Kaede, K., Ogata, E.: Optical consequences of blood substitution on tissue oxidation-reduction state microfluorometry. J.Appl.Physiol. 31, 93 (1971).

    PubMed  CAS  Google Scholar 

  7. Lang, H., Kessler, M., Starlinger, H.: Signs of hypoxia measured by means of Po2 multiwire electrodes by NADH and NADPH fluorescence and determination of lactate and pyruvate formation. In: Oxygen supply, theoretical and practical aspects of oxygen supply and microcirculation of tissue. Eds. M. Kessler, D.F. Bruley, L.C. Clark, D.W. Lubbers, I.A. Silver, J. Strauss, München-Berlin: Urban and Schwarzenberg 1973.

    Google Scholar 

  8. Lubbers, D.W.: personal communication.

    Google Scholar 

  9. Rahmer, H., Kessler, M.: Influence of hemoglobin concentration in perfusate and in blood on PN fluorescence of rat liver. In: International symposium on oxygen transport to tissue. April, 1973.

    Google Scholar 

  10. Sapirstein, L.A., Sapirstein, E.H., Bredemeyer, A.: Effect of hemorrhage on the cardiac output and its distribution in the rat. Circulation Res. 8, 135 (1960).

    Article  PubMed  CAS  Google Scholar 

  11. Schnitger, H., Scholz, R., Bücher, T., Lubbers, D.W.: Comparative fluorometric studies on rat liver in vivo and on isolated, perfused hemoglobin free liver. Biochem. Z. 341, 334 (1965).

    CAS  Google Scholar 

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Rink, R., Kessler, M., Hájek, K. (1973). Signs of Hypoxia in the Small Intestine of the Rat during Hemorrhagic Shock. In: Bicher, H.I., Bruley, D.F. (eds) Oxygen Transport to Tissue. Advances in Experimental Medicine and Biology, vol 37 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3288-6_59

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  • DOI: https://doi.org/10.1007/978-1-4684-3288-6_59

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-3290-9

  • Online ISBN: 978-1-4684-3288-6

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