Abstract
The major advantages of fluorometry as a quantitative analytical technique are generally recognized to be its high sensitivity and, to a lesser extent, its selectivity. For many fluorescent molecules the detection limits by fluorometry are at least two orders of magnitude less than those by absorption spectrophotometry. In some cases the selectivity of fluorometry may also be superior because of the more restricted scope of fluorescence (i.e., not all absorbing molecules fluoresce) and because of the ability of the analyst to select two wavelengths (excitation and emission) for the measurement of the fluorescing species.
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© 1976 Plenum Press, New York
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O’Haver, T.C. (1976). Modulation and Derivative Techniques in Luminescence Spectroscopy. In: Wehry, E.L. (eds) Modern Fluorescence Spectroscopy. Modern Analytical Chemistry, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2583-3_3
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DOI: https://doi.org/10.1007/978-1-4684-2583-3_3
Publisher Name: Springer, Boston, MA
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