Abstract
During the first fifty years of work with animal tissues and cells cultured in vitro, it has been clearly shown that the age of the donor determines the behavior of the explants. There are two basic phenomena which have been subjected to detailed experimental analysis. The first of these to be studied was the latent period, defined as the time after which cells first emigrate out of a freshly explanted tissue fragment. This period of dormancy increases with the increasing age of a normal tissue donor. The other phenomenon, which has been known for about 10 years, is the limited lifetime of diploid cells in culture. This lifespan is longer for cells cultured from embryos than for cells cultured from mature donors. Both phenomena have been studied in our laboratory. This paper deals with our findings concerning the relationship between the latent period of fragment cultures from different rat organs and age.
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© 1970 Plenum Press, New York
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Soukupová, M., Holečková, E., Hněvkovský, P. (1970). Changes of the Latent Period of Explanted Tissues During Ontogenesis. In: Holečková, E., Cristofalo, V.J. (eds) Aging in Cell and Tissue Culture. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1821-7_4
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DOI: https://doi.org/10.1007/978-1-4684-1821-7_4
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