Summary
Two methods are described for the determination of 8-methoxypsoralen (8-MPO; methoxsalen) in body fluids; a thin-layer densitometric method for qualitative and quantitative analysis in serum, and a high-performance liquid chromatographic method for the quantitative analysis in suction blister fluid and serum.
Some pharmacokinatic parameters of 8-MOP have been studied in psoriatic patients treated with this drug in combination with long-wave ultraviolet light irradiation. A surprising observation was, that 8-MOP serum concentrations increased after repeated oral administration. This phenomenon could not be ascribed to cumulation of the drug in the body.
The interlaboratory variation in 8-MOP assays was tested in two quality control samples. The results indicate that standardization of 8-MOP assay procedures is urgently needed.
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References
de Wolff, F.A. and van Kempen, G.M.J. (1976), “Determination of phenprocoumon, an anticoagulant, in human plasma.” Clin. Chem., 22, 1575–1578
Edelbroek, P.M. and de Wolff, F.A. (1978), “Improved micromethod for determination of underivatized clonazepam in serum by gas chromatography” Clin. Chem., 24, 1774–1777
Herfst, M.J., Koot-Gronsveld, E.A.M. and de Wolff, F.A. (1978), “Serum levels of 8-methoxypsoralen in psoriasis patients using a new fluorodensitometric method.” Arch. Derm. Res., 262, 1–6
Herfst, M.J. and van Rees, H. (1978), “Suction blister fluid as a model for interstitial fluid in rats.” Arch. Derm. Res., 263, 325–334
Parrish, J.A., Fitzpatrick, T.B., Tanenbaum, L. and Pathak, M.A. (1974), “Photochemotherapy of psoriasis with oral methoxypsoralen and longwave ultraviolet light”. New Engl, J. Med., 291, 1207–1211
Kempen, G.M.J., Koot-Gronsveld, E.A.M. and de Wolff, F.A. (1978), “Quantitative and qualitative analysis of the anticoagulant acenocoumarol in human plasma”. J. Chromatog. Biomed. Appl., 145, 332–335
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© 1980 International Association of Forensic Toxicologists
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de Wolff, F.A., Herst, M.J., Edelbroek, P.M. (1980). Determination of 8-Methoxypsoralen in Human Serum and Suction Blister Fluid. In: Oliver, J.S. (eds) Forensic Toxicology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1478-3_8
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DOI: https://doi.org/10.1007/978-1-4684-1478-3_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1480-6
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