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Effect of Temperature on Enzymatic Synthesis of Cephalosporins

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Bioorganic Chemistry in Healthcare and Technology

Part of the book series: NATO ASI Series ((NSSA,volume 207))

Summary

The effect of temperature on enzymatic synthesis of cephalexin (CEX) from 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) and D-alpha-phenyl glycine methyl ester (PGM) using immobilized penicillin G amidohydrolase (E.C.3.5.1.11) from E. coli ATCC 9637 was investigated. It was found that decreasing temperature results in a marked enhancement in both yield in condensation product and stability of catalyst. Analogous results were obtained using 7-amino-3-chloro (deacetoxymethyl) cephalosporanic acid as substrate for enzymatic synthesis of cefaclor.

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References

  1. T. Takahashi, Y. Yamazaki, K. Kato and M. Isono, J. Am. Chem. Soc., 1972, 94, 4035.

    Article  PubMed  CAS  Google Scholar 

  2. T. Fujii, K. Matsumoto and T. Watanabe, Process Biochem. 1976, 11, 21.

    Google Scholar 

  3. W. G. Choi, S. B. Lee and Dewey D. Y. Ryu, Biotechnol. Bioeng., 1981, 23, 361.

    Article  CAS  Google Scholar 

  4. V. Kashe, Biotechnol. Lett., 1985, 7, 877.

    Article  Google Scholar 

  5. Y. W. Ryu and Dewey D. Y. Ryu, Enzyme Microb. Technol., 1987, 9, 339.

    Article  CAS  Google Scholar 

  6. C. K. Hyun, J. H. Kim and Y. J. Kim, Biotechnol. Lett., 1989, 11, 537.

    Article  CAS  Google Scholar 

  7. D. H. Nam, C. Kim and Dewey D. Y. Ryu, Biotechnol. Bioeng., 1985, 27, 953.

    Article  PubMed  CAS  Google Scholar 

  8. J. Konecny, A. Schneider and M. Sieber, Biotechnol. Bioeng., 1983, 25, 451.

    Article  PubMed  CAS  Google Scholar 

  9. V. Kashe, Enzyme Microb. Technol., 1986, 8, 4.

    Article  Google Scholar 

  10. F. Knauseder and N. Palma, ‘3rd European Congress on Biotechnology, Munich’, Verlag Chemie, Weinheim, 1984, vol. 1, p. 431.

    Google Scholar 

  11. R. R. Chauvette, D. A. Pennington, J. Med. Chem. 1975, 18, 403.

    Article  PubMed  CAS  Google Scholar 

  12. V. Kashe, U. Haufler and L. Riechmann, Ann. N. Y. Acad. Sci., 1984, 434, 99.

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Biochemical Division De. Bi, Sclavo S.p.A., S. S. Padana Superiore km 160, 1-20060, Cassina de’ Pecchi (MI), Italy

    Eva M. Baldaro

Authors
  1. Eva M. Baldaro
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Editor information

Editors and Affiliations

  1. University of Amsterdam, Amsterdam, The Netherlands

    Upendra K. Pandit

  2. University of Antwerp (RUCA), Antwerp, Belgium

    Frank C. Alderweireldt

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© 1991 Plenum Press, New York

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Baldaro, E.M. (1991). Effect of Temperature on Enzymatic Synthesis of Cephalosporins. In: Pandit, U.K., Alderweireldt, F.C. (eds) Bioorganic Chemistry in Healthcare and Technology. NATO ASI Series, vol 207. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1354-0_21

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  • DOI: https://doi.org/10.1007/978-1-4684-1354-0_21

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-1356-4

  • Online ISBN: 978-1-4684-1354-0

  • eBook Packages: Springer Book Archive

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