Summary
We have previously shown that Mouse hepatitis virus (MHV) can undergo RNA-RNA recombination at a very high frequency (S. Makino, et al., J. Virol. 57, 729-737, 1986). To better define the mechanism of RNA recombination, we have performed additional crosses involving different MHV strains. We have obtained recombinant viruses with multiple cross-overs. The isolation of such recombinants further indicates the high frequency of coronavirus RNA recombination. By using cell fusion as a selection marker, we have also obtained recombinants between MHV-2 and A59 strains. Some of these recombinants have cross-overs in the 3′-end genes of the genome, thus demonstrating that recombination could occur along the entire genome. Finally, we have obtained recombinants by selecting with neutralizing monoclonal antibodies. These recombinants have cross-overs within gene C which encodes the peplomer protein. The genetic structure of these recombinants allowed us to determine the important domains of the peplomer proteins.
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© 1987 Plenum Press, New York
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Keck, J.G., Makino, S., Soe, L.H., Fleming, J.O., Stohlman, S.A., Lai, M.M.C. (1987). RNA Recombination of Coronavirus. In: Lai, M.M.C., Stohlman, S.A. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 218. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1280-2_11
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DOI: https://doi.org/10.1007/978-1-4684-1280-2_11
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