Abstract
The processes of membrane assembly and disassembly are major areas of interest in cell biology. One means of studying these processes is to add radioactive precursors of membrane components and then to measure the turnover of the radioactive molecules. The purpose of this paper is to report results for the turnover of l,2-diacyl-sn-glycero-3-phosphorylethanolamine (diacyl-GPE) and l,2-diacyl-sn-glycero-3-phosphorylcholine (diacyl-GPC) including the apparent presence of fast and slow pools, to describe the methods for calculations of half-lives and pool sizes when two or more pools are present, and to discuss the possible interpretations of the presence of two pools. Our results have been obtained with brains from mice after intracerebral injections of [14C]ethanolamine or [14C]glycerol.
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References
Ansell, G. B., Phospholipids and the nervous system, in Foim and Fmotion of Phospholipids, G. B. Ansell, R. M. C. Dawson and J. N. Hawthorne, eds., Elsevier Publishing Company, Amsterdam, 377–422 (1973).
Ansell, G. B. and Spanner, S., The magnesium-ion-dependent cleavage of the vinyl ether linkage of brain ethanolamine plasmalogen, Bioohem. J. 94:252–258 (1965).
Ansell, G. B. and Spanner, S., The metabolism of labeled ethanolamine in the brain of the rat in J. Neuroohem. 14:873–885 (1967).
Ansell, G. B. and Spanner, S., Plasmalogenase activity in normal and demyelinating tissue of the central nervous system, Biochem. J. 108:207–209 (1968a).
Ansell, G. B. and Spanner, S., The long-term metabolism of the ethanolamine moiety of rat brain myelin phospholipids, J.Neuro-ohem. 15:1371–1373 (1968b).
Ansell, G. B. and Spanner, S., The metabolism of [Me-14C]choline in the brain of the rat in vivo, Biochem. J. 110:201–206 (1968c).
Benjamins, J. A. and McKhann, G. M., [2-3H]glycerol as a precursor of phospholipids in rat brain: evidence for lack of recycling, J. Neurochem. 20:1111–1120 (1973a).
Benjamins, J. A. and McKhann, G. M., Properties and metabolism of soluble lipoproteins containing choline and ethanolamine phospholipids in rat brain, J. Neurochem. 20:1121–1129 (1973b).
Bunge, M. P., Bunge, R. P. and Ris, H., Ultrastructural study of remyelination in an experimental lesion in adult cat spinal cord, J. Biochem. Biophys. Qytol. 10:67–94 (1961).
Cohen, S. R. and Bemsohn, J., Incorporation of [1-14C] labeled fatty acids into isolated neuronal soma, astroglia and oligodendroglia from calf brain. Brain Res. 60:521–525 (1973).
Cooper, M. F. and Webster, G. R., The differentiation of phospholipase Al and A2 in rat and human nervous tissues, J. Neurochem. 17:1543–1554 (1970).
D’Amato, R. A., Horrocks, L. A. and Richardson, K. E., Kinetic properties of plasmalogenase from bovine brain, J. Neurochem. 24: 1251–1255 (1975).
Davison, A. N., Myelin metabolism, in Metabolism and Physiological Significance of Lipids, R. M. C. Dawson and D. N. Rhodes, eds. John Wiley and Sons, Ltd., London, 527–540 (1964).
Ellingson, J. S. and Lands, W. E. M., Phospholipid reactivation of plasmalogen metabolism. Lipids 3:111–120 (1968).
Gaiti, A., Amaducci, L., DeMedio, G. E., Brunetti, M. and Porcellati, G., Properties and function of calcium-dependent incorporation of choline, ethanolamine and serine into phospholipids of isolated rat brain microsomes,J. Neurochem. 23:1153–1159 (1974).
Gallai-Hatchard, J., Magee, W. L., Thompson, R. H. S. and Webster, G. R., The formation of lysophosphatides from diacyl phosphatides by brain preparations, J. Neurochem. 9:545–554 (1962).
Hill, E. E. and Lands, W. E. M., Phospholipid metabolism, in Lipid Metabolism, S. Wakil, ed. Academic Press, New York, 185–277 (1970).
Horrocks, L. A., Metabolism of the ethanolamine phosphoglycerides of mouse brain myelin and microsomes, J. Neurochem. 16:13–18 (1969).
Horrocks, L. A., The biosynthesis of plasmalogens from hexadecanol. Abstracts Third Meeting of the International Society for Neuro–chemistry, Budapest, 312 (1971).
Horrocks, L. A., Content, composition and metabolism of mammalian and avian lipids that contain ether groups, in Ether Lipids: Chemistry and Biologyy F. Snyder, ed. Academic Press, New York, 177–272 (1972).
Horrocks, L. A., Composition and metabolism of myelin phosphoglycerides during maturation and aging, Vrog. Brain Res., 40:383–395 (1973).
Horrocks, L. A. and Ansell, G. B., Studies on the phospholipids of rat brain which contain glycerol ethers,Biochim. Biophys. Acta 137:90–97 (1967).
Horrocks, L. A. and Sun, G. Y., Ethanolamine plasmalogens, in Researah Methods in Neuroohenristry, R. Rodnight and N. Marks, eds. Plenum Press, New York, 223–231 (1972).
Jungalwala, F. B., The turnover of myelin phosphatidylcholine and sphingomyelin in the adult rat brain. Brain Res. 78:99–108 (1974).
Jungalwala, F. B. and Dawson, R. M. C., The turnover of myelin phospholipids in the adult and developing rat brain, Bioohem. J. 123:683–693 (1971).
Kennedy, E. P. and Weiss, S. B., The function of cytidine coenzymes in the biosynthesis of phospholipids,J. Biol. Chem. 222:193–214 (1956).
Leibovitz-Ben Gershon, Z., Kobiler, I. and Gatt, S., Lysophospholipases of rat brain, J. Biol. Chem. 247:6840–6847 (1972).
McMurray, W. C. and Magee, W. L., Phospholipid metabolism, Annu. Rev. Bioohem. 41:129–160 (1972).
McMurray, W. C., Strickland, K. P., Berry, J. F. and Rossiter, R. H., Incorporation of 32P-labeled intermediates into the phospholipids of cell-free preparations of rat brain, Bioohem. J. 66: 634–644 (1957).
O’Brien, J. F. and Geison, R. L., Incorporation of 2-%-rglycerol into rat brain l,2-diacyl-sn-glycero-3-phosphorylcholine and 1,2-diacyl-sn-glycerol molecular species in vivo, J. Lipid Res. 15:44–49 (1974).
Pasquini, J. M., Krawiec, L. and Soto, E. F., Turnover of phosphatidylcholine in cell membranes of adult rat brain, J. Neuro-chem. 21:647–653 (1973).
Renooij, W. and Van Golde, L. M. G., Preferential incorporation of fatty acids at the inside of human erythrocyte membranes, Hoppe-Seyler’s Z. Physiol. Chem. 355:1242–1243 (1974).
Roberti, R., Binaglia, L., Francescangeli, E., Goracci, G. and Porcellati, G., Enzymic synthesis of l-alkyl-2-acyl-sn-glycero-3-phosphorylethanolamine through ethanolamine phosphotransferase activity in neuronal and glial cells of rabbit in vitro, Lipids 10:121–127 (1975).
Sabri, M. I., Bone, A. H. and Davison, A. N., Turnover of myelin and other structural proteins in the developing rat brain, Bioohem. J. 142:499–507 (1974).
Singer, S. J. and Nicolson, G. L., The fluid mosaic model of the structure of cell membranes, Soienoe 175:720–731 (1971).
Snyder, F., The enzymic pathways of ether-linked lipids and their precursors, in Ether Lipids: Chemistry and Biology F. Snyder, ed. Academic Press, New York, 121–156 (1972).
Sun, G. Y., Turnover of phosphoglycerides in subcellular fractions of mouse brain: study using [1-14C]-oleic acid as precursor, J. Neurochem. 21:1083–1092 (1973).
Sun, G. Y. and Horrocks, L. A., The incorporation of [14C]-palmitic acid into the lipids of mouse brain in vivo, J. Neuro-ahem. 18:1963–1969 (1971).
Sun, G. Y. and Horrocks, L. A., The metabolism of palmitic acid in the subcellular fractions of mouse brain, J. Lipid Res. 14:206–214 (1973).
Sun, G. Y., Winniczek, H., Go, J. and Sheng, S. L., Essential fatty acid deficiency-metabolism of 20:3 (n-9) and 22:2 (n-9) of major phosphoglycerides in subcellular fractions of developing and mature mouse brain.Lipids 10:365–373 (1975).
Van den Bosch, H., Phosphoglyceride metabolism, Annu. Rev. Biochem. 43:243–277 (1974).
Warner, H. R. and Lands, W. E. M., The metabolism of plasmalogen: enzymatic hydrolysis of the vinyl ether,J. Biol. Chem.. 236:2404–2409 (1961).
Webster, G. R., The incorporation of long-chain fatty acids into phospholipids of respiring slices of rat cerebrum, Biochem. J. 103:373–380 (1967).
Webster, G. R. and Cooper, M., On the site of action of phosphatide acylhydrolase activity of rat brain homogenates on lecithin, J. Neurochem. 15:795–802 (1968).
Wirtz, K. W. A., Transfer of phospholipids between membranes, Biochim. Biophys. Acta 344:95–117 (1974).
Woelk, H. and Porcellati, G., Subcellular distribution and kinetic properties of rat brain phospholipases A1 and A2, Hoppe-Seyler’s Z. Physiol. Chem. 354:90–100 (1973).
Woelk, H., Goracci, G., Gaiti, A. and Porcellati, G., Phospholipase Al and A2 activities of neuronal and glial cells of the rabbit brain, Hoppe-Seyler’s Z. Physiol. Chem. 354:729–736 (1973).
Yau, T. M. and Sun, G. Y., Incorporation of [1-14C]-oleic acid into neutral glycerides and phosphoglycerides of mouse brain. Lipids 8:410–414 (1973).
Yavin, E. and Gatt, S., Oxygen-dependent cleavage of the vinyl-ether linkage of plasmalogens. 1. Cleavage by rat brain supernatant, Eur. J. Biochem. 25:431–436 (1972).
Yavin, E. and Kanfer, J. N., Regulation of phospholipid metabolism in differentiating cells from rat brain cerebral hemispheres in culture. I. Uptake and phosphorylation of [U-14C]ethanolamine and the effect of various inhibitors, J. Biol. Chem. 250:2885–2890 (1975).
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Horrocks, L.A., Toews, A.D., Thompson, D.K., Chin, J.Y. (1976). Synthesis and Turnover of Brain Phosphoglycerides — Results, Methods of Calculation and Interpretation. In: Porcellati, G., Amaducci, L., Galli, C. (eds) Function and Metabolism of Phospholipids in the Central and Peripheral Nervous Systems. Advances in Experimental Medicine and Biology, vol 72. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-0955-0_5
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