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Multi Enzyme Systems in Membrane Reactors

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Enzyme Engineering

Abstract

Continuous operation is possible not only with carrier fixed enzymes but also with native (soluble) enzymes retained in reactors by means of an ultrafiltration membrane. This concept is especially useful for coenzyme depending multi enzyme systems, when the coenzyme also can be retained in the reactor. This is achieved by covalent coupling, e.g. NAD, to water soluble polyethylene glocol (PEG) of MW 10,000 to 40,000. Thus, L-leucine was produced from the corresponding a-keto acid at a productivity of 243 g/L/d for 24 days.

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References

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Author information

Authors and Affiliations

  1. Institute of Biotechnology, Nuclear Research Center, Juelich, Federal Republic of Germany

    C. Wandrey, R. Wichmann & A.-S. Jandel

Authors
  1. C. Wandrey
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  2. R. Wichmann
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  3. A.-S. Jandel
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Editor information

Editors and Affiliations

  1. Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Company, Osaka, Japan

    Ichiro Chibata

  2. Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Kyoto, Japan

    Saburo Fukui

  3. Department of Pharmacology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Lemuel B. Wingard Jr.

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© 1982 Plenum Press, New York

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Wandrey, C., Wichmann, R., Jandel, AS. (1982). Multi Enzyme Systems in Membrane Reactors. In: Chibata, I., Fukui, S., Wingard, L.B. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9290-7_5

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  • DOI: https://doi.org/10.1007/978-1-4615-9290-7_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9292-1

  • Online ISBN: 978-1-4615-9290-7

  • eBook Packages: Springer Book Archive

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