Abstract
We have recently reported that in the in vitro system of Mishell and Dutton (1) in which normally the 19s response is strongly T-cell dependent (2) the T-cell function can be replaced by a soluble factor (3). This soluble factor was derived from supernates of allogeneic spleen cells incubated for 24 hrs. and was called T-cell replacing factor, TRF. Our published data showed that the formation of TRF was dependent on the presence of T-cells and we suggested that TRF was actually a T-cell product. To substantiate this idea we now used allogeneic cells from different sources, that is, spleens, lymph nodes and thymus. Since the thymocytes contain a very small number of mature cells (4) which might be expected to participate in TRF production, the product derived from allogeneic thymocytes was fivefold concentrated.
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References
Mishell, R.J. and Button, R.W. J, Exp. Med. 126, 423 (1967).
Schimpl, A. and Wecker, E., Nature, 226, 1258 (1970).
Schimpl, A. and Wecker, E., Nature New Biology, 237, 15 (1972).
Lance, E.M. and Taub, R.N., Nature, 221, 84l (1969).
Dutton, R.W., Falkoff, R., Hirst, J.A., Hoffmann, U., Kappler, J.W., Kettman, J.F., Lesley, J.F. and Vann, D., in “Progress in Immunology”, 355 (ed. Bernard Amos, Academic Press 1971).
Taylor, R.B. and Wortis, H.S., Nature 220, 927 (1968).
Chan, E.L., Mishell, R.J. and Mitchell, G.F., Science 170, 1215 (1970).
Nordin, A.A., Cosenza, H. and Hopkins, W., J. Immunol. 103, 859 (1969).
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© 1973 Plenum Press, New York
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Schimpl, A., Wecker, E. (1973). Studies on the Source and Action of the T-Cell Replacing Factor (TRF). In: Janković, B.D., Isaković, K. (eds) Microenvironmental Aspects of Immunity. Advances in Experimental Medicine and Biology, vol 29. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9017-0_26
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DOI: https://doi.org/10.1007/978-1-4615-9017-0_26
Publisher Name: Springer, Boston, MA
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