Abstract
Poly(ADP-ribose) polymerase is a DNA-binding protein whose catalytic activity is stimulated strongly by DNA containing strand breaks (1). Recently, it has been proposed that a major function of the enzyme is to inactivate and eliminate cells with damaged DNA (2). DNA-binding proteins are established targets of autoimmunity in patients with systemic autoimmune diseases (3). Considering the possible functions of poly(ADP-ribose) polymerase in cells with DNA strand breaks, and the ability of the enzyme to undergo DNA-dependent automodification, it was conceivable that poly(ADP-ribose) polymerase could represent a potential autoantigen. For these reasons, we systematically searched for autoantibodies to poly(ADP-ribose) polymerase in human sera. Here we report the presence of autoantibodies to the poly(ADP-ribose) polymerase protein in patients with rheumatic diseases.
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Abbreviations
- ELISA:
-
enzyme-linked immunosorbent assay
- SDS:
-
sodium dodecyl sulfate
- PMSF:
-
phenylmethylsulfonyl fluoride
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© 1989 Springer-Verlag New York Inc.
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Yamanaka, H., Willis, E.H., Penning, C.A., Peebles, C.L., Tan, M., Carson, D.A. (1989). Characterization of the Human Autoantibody Response to Poly(ADP-Ribose) Polymerase. In: Jacobson, M.K., Jacobson, E.L. (eds) ADP-Ribose Transfer Reactions. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-8507-7_27
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DOI: https://doi.org/10.1007/978-1-4615-8507-7_27
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4615-8509-1
Online ISBN: 978-1-4615-8507-7
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