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Aging and Miniature Swine Heart and Liver Plasma Membranes

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Advances in Swine in Biomedical Research

Abstract

Age related changes in heart and liver plasma membranes were assessed over a wide age range, prepubertal, < 0.5 yr; young, 0.5 to 2.5 yr; middle aged, 5.9 to 10 yr; and old, 11.5 to 13.9 yr, in male and female Hormel-Hanford miniature swine fed the same energy restricted, low fat, cholesterol free, stock diet continuously post weaning. Mid bilayer heart plasma membrane fluidity, by diphenylhexatriene (DPH) polarization, was increased in old pigs, but fluidity in polar membrane domains was not increased in the old pigs. Age had no effect on fluidity by DPH in liver cell membranes, but surface domains were influenced by age. Liver cell membrane fluidity, by polar probes, followed a U shaped pattern with age and reached a maximum in the middle aged minipig group. In heart, middle aged pigs were reduced in total n-6 polyunsaturates, especially 18:2, and increased in saturates. Conversely, liver plasma membranes in middle aged pigs were elevated in unsaturated and reduced in saturated fatty acids. Heart membrane lipid peroxidation tended to be increased in middle aged and elderly pigs, perhaps due to increased membrane vitamin E in middle aged and old pigs. Thus, lipid peroxidation tended to increase over the lifespan of miniature swine, even when they were food restricted.

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Author information

Authors and Affiliations

  1. MNIL, BHNRC, ARS, USDA, Building 307, Rm. 323, BARC-East, Beltsville, MD, 20705, USA

    Dr. Elliott Berlin PhD, Melanie A. Banks PhD, Sam J. Bhathena PhD & Renee C. Peters BS

  2. Center for Food Safety and Applied Nutrition, Division of Toxicological Studies, Beltsville Research Facility, Food and Drug Administration, U.S. Department of Health and Human Services, Laurel, Maryland, 20708, USA

    Wesley A. Johnson DVM

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  1. Dr. Elliott Berlin PhD
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  4. Renee C. Peters BS
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  5. Wesley A. Johnson DVM
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Editors and Affiliations

  1. University of Illinois, Urbana, Illinois, USA

    Mike E. Tumbleson

  2. University of Minnesota, St. Paul, Minnesota, USA

    Lawrence B. Schook

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Berlin, E., Banks, M.A., Bhathena, S.J., Peters, R.C., Johnson, W.A. (1996). Aging and Miniature Swine Heart and Liver Plasma Membranes. In: Tumbleson, M.E., Schook, L.B. (eds) Advances in Swine in Biomedical Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5885-9_13

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  • DOI: https://doi.org/10.1007/978-1-4615-5885-9_13

  • Publisher Name: Springer, Boston, MA

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