Abstract
Some of the interactions between the porcine reproductive and respiratory syndrome virus (PRRSV) and the porcine interferon-α (IFN-α) system were studied. In a first experiment, it was shown that pretreatment of primary porcine alveolar macrophages (AMs) with recombinant porcine (rPo) IFN-α 1 resulted in significant reductions of PRRSV yield and numbers of antigen expressing cells. In a second experiment, sensitivity of PRRSV to IFN-α was confirmed in vivo. In pigs inoculated with porcine respiratory Coronavirus (PRCV)—a potent inducer of endogenous IFN-α in the lungs of pigs—followed 2 days later by PRRSV—lung PRRSV titers were 1.7 to 2.9 log10 TCID50 reduced compared to those in singly PRRSV inoculated pigs. It was concluded therefore that PRRSV has a fairly good sensitivity to the antiviral effects of IFN-α. A third experiment documented that in vivo PRRSV infection generally does not affect PRCV-induced IFN-α production in the lungs of pigs. In addition, it was shown that the IFN-inducing capacity of PRRSV is at least 159 times lower than that of PRCV. This finding suggests that cells other than AMs may be responsible for IFN production in the lungs of pigs.
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Keywords
- African Swine Fever Virus
- Respiratory Syndrome Virus
- Recombinant Porcine
- Transmissible Porcine Gastroenteritis Virus
- Porcine Respiratory Coronavirus
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Albina, E., Carrat, C, and Charley, B., 1997, Interferon alpha response to porcine reproductive and respiratory syndrome virus (PRRSV), J. Gen. Virol., submitted.
Artursson, K., Wallgren, P., and Alm, G., 1989, Appearance of interferon-a in serum and signs of reduced immune function in pigs after transport and installation in a fattening farm, Vet. Immunol. Immunopathol. 23: 345–353.
Babiuk, L. A., Bielefeldt Ohmann, H., Gifford, G., Czarniecki, C. W., Scialli, V. T., and Hamilton, E. B., 1985, J. Gen. Virol. 66: 2383–2394.
Charley, B., and Lavenant, L.,1990, Characterization of blood mononuclear cells producing IFN-α following induction by coronavirus-infected cells (transmissible porcine gastroenteritis virus). Res. Immunol. 141: 141.
Duan, X., Nauwynck, H.J., and Pensaert, M.B.,1997, Virus quantification and identification of cellular targets in the lungs and lymphoid tissues of pigs at different time intervals after inoculation with porcine reproductive and respiratory syndrome virus (PRRSV), Vet. Microbiol. 56: 9–19.
Esparza, I., Gonzalez, J. C, and Vinuela, E., 1988, Effect of interferon-α, interferon-y and tumour necrosis factor-α on African swine fever virus replication in porcine monocytes and macrophages, J. Gen. Virol. 69: 2973–2980.
Holland, S. P., Fulton, R. W., Short, E. C, Wyckoff, J. H., and Fox, J. C, 1991, In vitro and in vivo 2′ 5′-oli-goadenylate synthetase activity induced by recombinant DNA-derived bovine interferon al 1 in bovine alveolar macrophages and blood mononuclear cells, Am. J. Vet. Res. 52: 1779–1783.
Iglesias, G., Pijoan, C, and Molitor, T., 1992, Effects of Pseudorabies virus infection upon cytotoxicity and antiviral activities of porcine alveolar macrophages, Comp. Immunol. Microbiol. Infect. Dis. 15: 249–259.
Lefèvre, F., L’Haridon, R., Borras-Cuesta, F., and La Bonnardière, C, 1990, Production, purification and biological properties of an Escherichia coli-derived recombinant porcine alpha interferon, J. Gen. Virol. 71: 1057–1063.
Powell, P.P., Dixon, L.K. and Parkhouse, R.M., 1996, An IkappaB homolog encoded by African swine fever virus provides a novel mechnanism for downregulation of proinflammatory cytokine responses in host macrophages, J. Virol. 70: 8527–8533.
Saksela, E., Virtanen, I., Hovi, T., Secher, D.S., and Cantell, K., 1984, Monocyte is the main producer of human alpha interferons following Sendai virus induction, Prog. Med. Virol. 30: 78.
Van Reeth, K., and Pensaert, M.B., 1994, Porcine respiratory coronavirus-mediated interference against influenza virus replication in the respiratory tract of feeder pigs, Am. J. Vet. Res. 55: 1275–1281.
Van Reeth, K., and Pensaert, M., 1995, Production of interferon-a, tumor necrosis factor-α and interleukin-1 in the lungs of pigs infected with the porcine respiratory Coronavirus. Proc. 3rd Congress Europ. Soc. Vet. Virol., Interlaken, Switzerland, 197–201.
Van Reeth, K., and Pensaert, M., 1996, A clinical and virological study in pigs infected with Aujeszky’s disease virus shortly after infection with porcine respiratory Coronavirus. Proc. 14th Congress Int. Pig Vet. Soc, Bologna, Italy, 137.
Wensvoort, G., Terpstra, C, Pol, J.M.A., ter Laak, E.A., Bloemraad, M., de Kluyver, E.P., Kragten, C, van Buiten, L., den Besten, A., Wagenaar, F., Broekhuijsen, J.M., Moonen, P.L.J.M., Zetstra, T., de Boer, E.A., Tibben, HJ., de Jong, M.F., van Veld, P., Groenland, G.J.R., van Gennep, J.A., Voets, M.T., Verheijden, J.H.M., and Braanskamp, J., 1991, Mystery swine disease in The Netherlands: the isolation of the Lelystad virus, Vet. Q. 13: 121–130.
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Buddaert, W., Van Reeth, K., Pensaert, M. (1998). In Vivo and In Vitro Interferon (IFN) Studies with the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_59
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