Abstract
Infection of mice with Coronavirus mouse hepatitis virus strain MHV-A59 causes focal acute encephalitis, hepatitis and chronic demyelinating disease. To investigate host in-terferon (IFN) response to viral infection within the brain, RNA was extracted from A59-or MHV-2- infected and mock-infected primary astrocyte cultures from newborn mice, RT-PCR amplified RNA with primers specific for the various IFNs, transferred to nylon membranes and hybridized with IFN specific digoxigenin-labeled probes. Infection of primary astrocyte cultures from newborn mice with either A59 or MHV-2 caused upregulation of IFN-ß RNA, but not IFN-γ or IFN-α. Thus, brain astrocytes are capable of producing a local IFN-ß response upon infection with MHV. The response of the other IFNs following MHV infection may be derived from inflammatory cells.
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Wang, Q., Haluskey, J.A., Lavi, E. (1998). Coronavirus MHV-A59 Causes Upregulation of Interferon-ß RNA in Primary Glial Cell Cultures. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_57
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DOI: https://doi.org/10.1007/978-1-4615-5331-1_57
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