Abstract
Methods to develop an immuno-optical biosensor for the detection and monitoring of Protein C (PC) concentrations are described. A tapered quartz fiber is enclosed in a glass tube (capacity Ü300 μI) and monoclonal antibody against PC (anti-PC) is immobilized on the surface of this fiber. PC within a sample, when injected into the chamber, will bind to the anti-PC in a specific reaction. The system is then probed with a fluorophore tagged secondary antibody against PC, also binding to PC in a specific reaction. Excitation light is applied through the fiber, and the amount of fluorescence is correlated with the PC concentration in the sample. This study offers encouraging results for the detection of PC deficiency in real-time.
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References
Bhatia, S.K., Shriver-Laker, L.C., Prior, K.J., Georger, J.H., Calvert, J.M., Bredehorst, R., and Ligler F.S., “Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces,” Analytical Biochemistry, 178,408–413, 1989.
Bruley, D.F. and Drohan, W.N., Protein C and Related Anticoagulants—Advances in Applied Biotechnology Series, 11, Gulf Publishing Company, Houston, 1990.
Kang, K.A., Ryu, D., Drohan, W.D., and Orthner, C.L., “Effect of Matrices on the Immunoaffmity Purification of Protein C,” Biotech. & Bioengr, 39(11), 1086–1098, 1992.
Spiker, J.O., Kang, K.A., Drohan, W.N., Bruley, D.F., “Protein C Detection Via Fluorophore Mediated Immuno-Optical Biosensor,” ISOTT XXIV, Advances in Experimental Medicine and Biology, in press.
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© 1998 Springer Science+Business Media New York
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Spiker, J.O., Kang, K.A., Drohan, W.N., Bruley, D.F. (1998). Preliminary Study of Biosensor Optimization for the Detection of Protein C. In: Hudetz, A.G., Bruley, D.F. (eds) Oxygen Transport to Tissue XX. Advances in Experimental Medicine and Biology, vol 454. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4863-8_81
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DOI: https://doi.org/10.1007/978-1-4615-4863-8_81
Publisher Name: Springer, Boston, MA
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