Abstract
The mammary fat pad, when cleared of glandular tissues in female mice 3–4 weeks old, provides an ideal site for the transplantation of normal, hyperplastic, or malignant lesions of the mammary gland into syngeneic hosts. The cleared fat pad retains the microenvironment necessary for the normal morphological growth of transplanted mammary gland elements. The biological development of mammary gland structures can be evaluated and lesions can be tested for cancer risk. Serial transplantation into cleared fat pads can provide pools of the tissue of choice for biochemical, immunological, and molecular analysis, for tissue culture, and for the perpetuation of hyperplastic tissues for future use. With the advent of transgenic and knockout mice, the clearing technique has become a powerful biological system wherein the effect of genes and their interactions on mammary neoplasia can be determined. This chapter describes the clearing and transplantation technique for mammary tissues and cells.
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Young, L.J.T. (2000). The Cleared Mammary Fat Pad and the Transplantation of Mammary Gland Morphological Structures and Cells. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_6
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DOI: https://doi.org/10.1007/978-1-4615-4295-7_6
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