Abstract
We describe a method to transplant embryonic mammary anlagen into the mammary fat pad of a virgin host whose endogenous mammary epithelium has been removed. The transplanted epithelium grows into the fat pad and is exposed to a physiological hormonal milieu of puberty and pregnancy when the host is mated. This technique allows studies on the developmental potential of mammary epithelia of mutants that result in lethality after embryonic day 13. Transplanted mammary epithelial cells derived from insulin-receptor-deficient mice show reduced size of alveoli at lactation and reduced expression of milk protein genes. These results underscore the importance of insulin receptor signaling in mammary epithelial development.
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Abbreviations
- DMEM:
-
Dulbecco’s modified Eagle’s medium
- FCS:
-
fetal calf serum
- IGF:
-
insulin-like growth factor
- IR:
-
insulin receptor
- PBS:
-
phosphate buffered saline
- PCR:
-
polymerase chain reaction
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Robinson, G.W., Accili, D., Hennighausen, L. (2000). Rescue of Mammary Epithelium of Early Lethal Phenotypes by Embryonic Mammary Gland Transplantation as Exemplified with Insulin Receptor Null Mice. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_26
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DOI: https://doi.org/10.1007/978-1-4615-4295-7_26
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