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Methods for the Induction of Mammary Carcinogenesis in the Rat Using either 7,12-Dimethylbenz[α]anthracene or 1-Methyl-1-Nitrosourea

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Methods in Mammary Gland Biology and Breast Cancer Research

Abstract

Numerous animal model systems for investigating breast cancer exist, and new experimental approaches using transgenic and knockout strategies are resulting in the development of models with specific pathogenetic characteristics. Nonetheless, chemically induced mammary carcinogenesis has been investigated for over 50 years, and the results of experiments using this approach continue to provide important insights about the genesis, prevention, and treatment of breast cancer. This chapter provides a detailed account of the methods utilized to implement the two most widely studied chemically initiated models of mammary carcinogenesis in the rat. They are induced by treatment of female rats with either 7,12-dimethylbenz[α]anthracene or 1-methyl-1-nitrosourea.

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Abbreviations

IBC:

Institutional Biohazard Committee

IACUC:

Institutional Animal Care and Use Committee

DMBA:

7,12-dimethylbenz[α]anthracene

MNU:

1-methyl-l-nitrosourea

References

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Author information

Authors and Affiliations

  1. Center for Nutrition in the Prevention of Disease, AMC Cancer Research Center, Lakewood, Colorado, 80214, USA

    Henry J. Thompson

Authors
  1. Henry J. Thompson
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Editor information

Editors and Affiliations

  1. Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, New York, 14263, USA

    Margot M. Ip

  2. Roswell Park Cancer Research Institute, Buffalo, New York, USA

    Bonnie B. Asch

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© 2000 Springer Science+Business Media New York

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Thompson, H.J. (2000). Methods for the Induction of Mammary Carcinogenesis in the Rat Using either 7,12-Dimethylbenz[α]anthracene or 1-Methyl-1-Nitrosourea. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_2

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  • DOI: https://doi.org/10.1007/978-1-4615-4295-7_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6927-1

  • Online ISBN: 978-1-4615-4295-7

  • eBook Packages: Springer Book Archive

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