Abstract
Whole mammary glands from pubescent mice, primed for 9 days with estrogen and progesterone, cultured in the presence of the lactogenic hormone mix of insulin, aldosterone, hydrocortisone, and prolactin develop pregnancy-like lobuloalveolar structures and synthesize milk protein. Withdrawal of prolactin from the medium (with or without the steroids) results in involution of the gland in a manner comparable to that which occurs in vivo. Reincubation with the four-hormone mix plus epidermal growth factor results in a second round of development. Thus, whole organ culture provides an excellent model system to study systematically the hormonal and growth factor requirements that regulate the biochemical events that occur during these processes.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Abbreviations
- (I):
-
insulin
- (E):
-
estradiol
- (P):
-
progesterone
- (H):
-
hydrocortisone
- (A):
-
aldosterone
- (PRL):
-
prolactin
- (EGF):
-
epidermal growth factor
- (PBS):
-
phosphate buffered saline
References
R. R. Ichinose and S. Nandi (1964). Lobuloalveolar differentiation in mouse mammary tissues in vitro. Science 145: 496–497.
R. R. Ichinose and S. Nandi (1966). Influence of hormones on lobulo-alveolar differentiation of mouse mammary glands in vitro. J. Endocrin. 35: 331–340.
P. M. Terry, M. R. Banerjee, and R. M. Lui (1977). Hormone-inducible casein messenger RNA in a serum-free organ culture of whole mammary gland. Proc. Natl. Acad. Sci. USA 74: 2441–2445.
B. K. Vonderhaar, Hormones and growth factors in mammary gland development. In C. M. Veneziale (ed.), Control of Cell Growth and Proliferation, 1984, Van Nostrand, Reinhold, New York, pp. 11–33.
K. Plaut, M. Ikeda, and B. K. Vonderhaar (1993). Role of growth hormone and insulin-like growth factor I in mammary development. Endocrinology. 133: 1843–1848.
B. G. Wood, L. L. Washburn, A. S. Mukherjee, and M. R. Banerjee (1975). Hormonal regulation of lobulo-alveolar growth, functional differentiation and regression of whole mouse mammary gland in organ culture. J. Endocrinol. 65: 1–6.
Q. J. Tonelli and S. Sorof (1980). Epidermal growth factor requirement for development of cultured mammary gland. Nature 285: 250–252.
C. S. Atwood, M. Ikeda, and B. K. Vonderhaar (1995). Involution of mouse mammary glands in whole organ culture: a model for studying programmed cell death. Biochem. Biophys. Res. Commun. 207: 860–867.
C. S. Atwood, R. C. Hovey, J. P. Glover, G. Chepko, E. Ginsburg, E. Robinson, Jr., and B. K. Vonderhaar (2000). Progesterone induces ductal side branching in the mammary glands of pubertal mice. J. Endocrinol. (in press)
R. A. Knazek, S. C. Liu, J. S. Bodwin, and B. K. Vonderhaar (1980). Essential fatty acids are required in the diet for development of the mouse mammary gland. J. Natl. Cancer Inst. 64: 377–382.
H. Michna, Y. Nishino, M. R. Schneider, T. Louton, and M. F. elEtreby (1991). A bioassay for the evaluation of antiproliferative potencies of progesterone antagonists. J. Steroid Biochem. Mol. Biol. 38: 359–365.
J. Barlow, T. Casey, J. E Chiu, and K. Plaut (1997). Estrogen affects development of alveolar structures in whole-organ culture of mouse mammary glands. Biochem. Biophys. Res. Commun. 232: 340–344.
K. Plaut, R. Maple, E. Ginsburg, and B. Vonderhaar (1999). Progesterone stimulates DNA synthesis and lobulo-alveolar development in mammary glands in ovariectomized mice. J. Cell. Physiol. 180: 298–304.
W. G. Dilley and S. Nandi (1968). Rat mammary gland differentiation in vitro in the absence of steroids. Science 161: 59–60.
D. V. Singh, K. B. DeOme, and H. A. Bern (1970). Strain differences in response of the mouse mammary gland to hormones in vitro. J. Natl. Cancer Inst. 45: 657–675.
M. R. Banerjee, B. G. Wood, and D. L. Kinder (1973). Whole mammary gland organ culture: selection of appropriate gland. In Vitro 9: 129–133.
D. V. Singh and H. A. Bern (1969). Interaction between prolactin and thyroxine in mouse mammary gland lobulo-alveolar development in vitro. J. Endocrinol. 45: 579–583.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer Science+Business Media New York
About this chapter
Cite this chapter
Ginsburg, E., Vonderhaar, B.K. (2000). Whole Organ Culture of the Mouse Mammary Gland. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_14
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4295-7_14
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6927-1
Online ISBN: 978-1-4615-4295-7
eBook Packages: Springer Book Archive