Abstract
The monoclonal antibody (MAb) NKI-C3 (Netherlands Cancer Institute) is a murine IgGl known to react with a formalin resistant antigen expressed by more than 95% of human melanomas. In principle, it can be used in the diagnosis of tissue sections for the presence of melanocytes, and with the appropriate level of boron labelling, this antibody could also be used for NCT of melanoma, provided its specificity is not impaired by the labelling process. The present study involves the use of two imaging techniques: first, α-track etch autoradiography to determine the specifiCity of the MAb after boronation, and second, electron energy loss spectroscopy to obtain information at the subcellular level concerning the localisation of the boron label.
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References
F Alam, AH Soloway, RF Barth, N Mafune, DM Adams, WH Knoth. Boron neutron capture therapy: Linkage of a boronated macromolecule to monoclonal antibodies directed against tumour-associated antigens. J. Med. Chem., 32, 2326–2330, 1989.
SR Tamat, DE Moore, A Patwardhan, P Hersey. Boronated monoclonal antibody 225.28S for potential use in neutron capture therapy of malignant melanoma. Pigment Cell Res., 2, 278–280, 1989.
SR Tamat, DE Moore, BJ Allen. Assay of boron in biological tissues by inductively coupled plasma atomic absorption spectrometry. Anal. Chem., 59, 2161–2164, 1987.
RD Leapman, RL Ornberg. Quantitative electron energy loss spectroscopy in biology, Ultramicroscopy, 24, 251–268, 1988.
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© 1992 Springer Science+Business Media New York
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Moore, D.E., Stretch, J.R., Dawes, A.L., Cockayne, D.J.H., Allen, B.J., Constantine, G. (1992). Uptake of Boronated Monoclonal Antibodies by Melanoma Cells Visualised by Track Etch Autoradiography and Electron Energy Loss Spectroscopy. In: Allen, B.J., Moore, D.E., Harrington, B.V. (eds) Progress in Neutron Capture Therapy for Cancer. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3384-9_73
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DOI: https://doi.org/10.1007/978-1-4615-3384-9_73
Publisher Name: Springer, Boston, MA
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Online ISBN: 978-1-4615-3384-9
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