Summary
The exchangeability of the bacteriopheophytins at sites HA and HB with modified (bacterio)pheophytins (=(B)Phe)*) was tested in reaction centers (RC) of Rhodobacter spheroides R26. An exchange at both sites is possible with Pyro-BPhe a lacking the 132-COOCH3 group, and with three plant-type pheophytins: Phe a (which contains a 3-vinyl-group), 132-hydroxy-Phe a (which contains in addition a hydroxy group), and [3-acetyl]-Phe a (which differs from BPhe a only by the unsaturated ring II). In all cases, the exchange appears to be easier at the HB -site. An exchange only at this site, was obtained with BPhe a gg in which the esterifying phytol is replaced by geranyl-geraniol, and with 132-hydroxy-Phe a. Environment-induced red-shifts (EIRS) are observed with all pigments, and they are in the range of the ones known for the native BPhe a. Strong optical activity is induced in most pigments. Shifts in the absorptions of the monomeric BChls at sites BA,B indicate an interaction with the BPhes sites HA,B or an indirect structural effect.
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Abbreviations
- Chl :
-
chlorophyll
- Phe :
-
pheophytin
- BChl :
-
bacterio-chlorophyll
- BPhe :
-
bacteriopheophytin; the subscripts refer to the esterifying alcohols (“p” or none for phytol, “gg” for geranylgera-niol)
- RC:
-
reaction centers
- Rb. :
-
Rhodobacter
- cd:
-
circular dichroism
- P:
-
primary donor site
- B:
-
site of monomeric BChl
- H:
-
site of BPhe in RC. The subscripts “A” and “B” refer to the active (“L”) and inactive branch (“M”), respectively, of the electron transport chain
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Scheer, H., Meyer, M., Katheder, I. (1992). Bacterial Reaction Centers with Plant-Type Pheophytins. In: Breton, J., Verméglio, A. (eds) The Photosynthetic Bacterial Reaction Center II. Nato ASI Series, vol 237. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3050-3_7
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DOI: https://doi.org/10.1007/978-1-4615-3050-3_7
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