Abstract
The nucleotide (nt) sequence of 1.7 kbp cDNA representing the 3’ end of the PEDV genome has been determined. Viral RNA was reverse transcribed and the cDNA was amplified by polymerase chain reaction using degenerate primers. The sequences of the primers were based on conserved regions of coronaviral genomes. A 1323 nt open reading frame (ORF) showed good homology to the nucleocapsid (N) gene of other coronaviruses. The greatest homologies at the amino acid and the nt levels were observed with Human Coronavirus 229E. A second 336 nt ORF, which might encode a leucine-rich protein, was found within the N gene. Between the 3’ end of the N gene and the poly(A) tail was a sequence of eleven nt, which is conserved among the other sequenced coronaviruses. Finally, a seven base sequence similar to the conserved intergenic sequences was present 5’ to the N gene. These results confirm the classification of PEDV as a coronavirus.
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Keywords
- Porcine Epidemic Diarrhoea Virus
- Infectious Bronchitis Virus
- Nucleocapsid Protein
- Intergenic Sequence
- Feline Infectious Peritonitis Virus
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Z. Yaling, J. Ederveen, H. Egberink, M. Pensaert, and M.C. Horzinek, Arch. Virol. 102:63 (1988).
M.B. Pensaert and P. Debouck, Arch. Virol. 58:243 (1978).
M. Hofmann and R. Wyler, J. Clin. Microbiol. 26:2235 (1988).
M. Knuchel, M. Ackermann, H.K. Müller, and U. Kihm, Vet. Microbiol. 32:117 (1992).
R.E. Kingston, “Current Protocols in Molecular Biology,” section 4.1.2, Greene Publishing Associates, Brooklyn (1991).
U.V. Wirth, B. Vogt, and M. Schwyzer, J. Virology 65:195 (1991).
A. Bridgen, K. Tobler, and M. Ackermann, Adv. Exp. Med. Biol., (this volume).
M.A. Frohman, M.K. Dush, and G.R. Martin, Proc. Natl. Acad. Sci. 85:8998 (1988).
T. Maniatis, E.F. Fritsch, and J. Sambrook. “Molecular Cloning: A Laboratory Manual (2nd edition),” Cold Spring Harbour Laboratory, Cold Spring Harbor NY (1989).
J. Devereux, P. Haeberli, and O. Smithies, Nucl. Acids Res. 12:387 (1984).
H.F.E. Egberink, J. Ederveen, P. Callebaut, and M.C. Horzinek, Am. J. Vet. Res. 49:1320 (1988)
S.S. Schreiber, T. Kamahora, and M.C. Lai, Virology 169:142 (1989).
P.A. Kapke and D.A. Brian, Virology 151:41 (1986).
H. Vennema, R.J. De Groot, D.A. Harbour, M.C. Horzinek, and W.J.M. Spaan, Virology 181:327 (1991).
M.M. Parker and P.S. Master, Virology 179:463 (1990).
T. Kamahora, L.H. Soe, and M.C. Lai, Virus Research 12:1 (1989).
W. Lapps, B.G. Hogue, and D.A. Brian, Virology 157:47 (1987).
M.E.G. Boursnell, M.M. Binns, I.J. Fould, and T.D.K. Brown, J. Gen. Virol. 66:573 (1985).
M. Kozak, Cell 44:283 (1986).
S.D. Senanayake, M.A. Hofmann, J.L. Maki, and D.A. Brian, J. Virol. 66:5277 (1992).
M.C. Lai, Ann. Rev. Microbiol. 44:303 (1990).
D. Smoller, C. Friedel, A. Schmid, D. Bettler, L. Lam, and D. Yedvobnick, Genes Dev. 4:1688 (1990).
R.J. Roller and B. Roizman, J. Virol. 66:3624 (1992).
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Tobler, K., Bridgen, A., Ackermann, M. (1994). Sequence Analysis of the Nucleocapsid Protein Gene of Porcine Epidemic Diarrhoea Virus. In: Laude, H., Vautherot, JF. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 342. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2996-5_8
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