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Physical Characteristics and Polymerization During Iron Saturation of Lactoferrin, A Myelopoietic Regulatory Molecule with Suppressor Activity

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Lactoferrin

Part of the book series: Advances in, Experimental Medicine and Biology ((AEMB,volume 357))

Abstract

Lactoferrin (LF) has been implicated in normal regulation of myeloid blood cell production in vitro and in vivo and abnormalities in LF-cell interactions have been associated with progression of leukemia and other hematopoietic disorders. LF may be clinically useful and for this reason we studied selected biochemical characteristics of LF. Purified human milk LF was saturated with iron from solution and analyzed by gel electrophoresis, ion-exchange and gel filtration chromatography. The metalloprotein was found to contain several molecular weight species on polyacrylamide gels. High resolution ion-exchange chromatography demonstrated the binding of LF to both anionic and cationic media under identical conditions indicating a bipolar charge distribution. Gel filtration studies revealed a tetramerized form of LF, the formation and stability of which was dependent on the ionic strength of the solution.

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Author information

Authors and Affiliations

  1. Walther Oncology Center and Department of Medicine (Hematology/Oncology), Indianapolis, Indiana, 46202, USA

    Charlie Mantel, Keisuke Miyazawa & Hal E. Broxmeyer

  2. Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, 46202, USA

    Hal E. Broxmeyer

Authors
  1. Charlie Mantel
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  2. Keisuke Miyazawa
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  3. Hal E. Broxmeyer
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Editor information

Editors and Affiliations

  1. University of California, Davis, Davis, California, USA

    T. William Hutchens  & Bo Lönnerdal  & 

  2. Massey University, Palmerston North, New Zealand

    Sylvia V. Rumball

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© 1994 Springer Science+Business Media New York

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Mantel, C., Miyazawa, K., Broxmeyer, H.E. (1994). Physical Characteristics and Polymerization During Iron Saturation of Lactoferrin, A Myelopoietic Regulatory Molecule with Suppressor Activity. In: Hutchens, T.W., Rumball, S.V., Lönnerdal, B. (eds) Lactoferrin. Advances in, Experimental Medicine and Biology, vol 357. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2548-6_12

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  • DOI: https://doi.org/10.1007/978-1-4615-2548-6_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6087-2

  • Online ISBN: 978-1-4615-2548-6

  • eBook Packages: Springer Book Archive

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