Abstract
Since the introduction of more dedicated methods in blood gas analysis a lot of confusion started about definition and notation on oxygen related quantities in blood. The (US) National Committee for Clinical Laboratory Standards (NCCLS) has published a proposed guideline in which these problems are thoroughly discussed18. In the present paper a consistent set of definitions is given of the principal quantities pertaining to oxygen in blood in relation to the methods employed in the measurement of the quantities. Its core is the correct definition of oxygen saturation of hemoglobin as given in equations [3], [6], and [12a], which is in agreement with that given by NCCLS19. This system at least is consistent and the arguments are presented in this paper in a number of statements. The core of it is the correct definition on oxygen saturation of hemoglobin as given in and around equation [3].
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References
Christoforides C, Hedley-White J. Effect of temperature and hemoglobin concentration on the solubility of O2 in blood. J Appl Physiol 27, 592–59106, 1969.
Roughton FJW, Severinghaus JW. Accurate determination of O2 dissociation curve of human blood above 98.7% saturation with data on O2 solubility in unmodified human blood from 0 to 37 °C. J Appl Physiol 35, 861–869, 1973.
VanSlyke DD, Neill JM. Determination of gases in blood and other solutions by vacuum extraction and manometric measurement. J Biol Chem 61, 523–573, 1924.
Zwart A, Kwant G, Oeseburg B, Zijlstra WG. Oxygen dissociation curves for whole blood, recorded with an instrument that continously measures pO2 and sO2 independently at constant T, pCO2, and pH. Clin Chem 28, 1287–1292, 1982.
Wimberley PD, Burnett RW, Covington AK, Fogh-Andersen N, Maas AHJ, MüllerPlathe O, Siggaard-Andersen O, Zijlstra WG. Guidelines for routine measurement of blood hemoglobin oxygen affinity. Scand J Clin Lab Invest 50, suppl 203, 227–234, 1990.
Dijkhuizen P, Buursma A, Fongers TME, Gerding AM, Oeseburg B, Zijlstra WG. The oxygen binding capacity of human haemoglobin. Hüffner’s factor redetermined. Pflügers Arch 369, 223–231, 1977.
Zwart A, van Kampen ET, Zijlstra WG. Results of routine determination of clinically significant hemoglobin derivatives by multicomponent analysis. Clin Chem 32, 972–978, 1986.
International Committee for Standardization in Haematology. Recommendations for reference method for haemoglobinometry in human blood (ICSH standard 1986) and specifications for international haemiglobincyanide reference preparation (3rd edition). Clin lab Haemat 9, 73–79, 1987.
Van Kampen EJ, Zijlstra WG. Spectrophotometry of hemoglobin and hemoglobin derivatives. Adv Clin Chem 23, 199–257, 1983.
Braunitzer G, Gehring-Müller R, Hilschmann N, Hilse K, Hobom G, Rudolf V, Wittmann-Liebold B. Die Konstitution des normalen adulten Humanhämoglobins. Hoppe Seylers Z Physiol Chem 325, 283–288, 1961.
Brinkman R, Zijlstra WG. Determination and continuous registration of the percentage oxygen saturation in clinical condition. Arch Chir Neerl 1, 177–183, 1949.
Zijlstra WG, Buursma A, Meeuwsen-van der Roest WP. Absorption spectra of human fetal and adult oxyhemoglobin, de-oxyhemoglobin, carboxyhemoglobin, and methemoglobin. Clin Chem 37, 1633–1638, 1991.
Zwart A, Buursma A, Van Kampen EJ, Zijlstra WG. Multicomponent analysis of hemoglobin derivatives with a reversed-optics spectrophotometer. Clin Chem 30, 373–379, 1984.
Zwart A, Buursma A, Oeseburg B, Zijlstra WG. Determination of hemoglobin derivatives with the IL 282 CO-oximeter as compared with a manual spectrophotometric five-wavelength method. Clin Chem 27, 1903–1907, 1981.
Zijlstra WG, Buursma A, Zwart A. Performance of an automated six-wavelength photometer (Radiometer OSM 3) for routine measurement of hemoglobin derivatives. Clin Chem 34, 149–152, 1988.
Zijlstra WG. Quantitative evaluation of the oxygen transport capability of human blood. Pflügers Arch 408, S9–S10, 1987.
Zijlstra WG, Oeseburg B. Definition and notation of hemoglobin oxygen saturation. IEEE Trans Biomed Eng 36, 872, 1989.
Moran RF, Clausen JL, Ehrmeyer S, Feil M, Van Kessel AL, Eichhorn JH. Oxygen content, hemoglobin oxygen saturation, and related quantities in blood: terminology, measurement, and reporting. NCCLS Document C25-P. Vol 10, No 2, 1990.
Ehrmeyer S, Burnett RW, Chatburn RL, Christiansen TF, Clausen JL, Cormier AD, Durst RA, Eichhorn JH, Fallon KD, Moran RG, Van Kessel AL. Definitions of quantities and conventions related to blood pH and gas analysis. NCCLS Document C12–T2. Vol 11, No 18, 1991.
Wimberley PD, Siggaard-Andersen O, Fogh-Andersen N, Zijlstra WG, Severinghaus JW. Haemoglobin oxygen saturation and related quantities: definitions, symbols and clinical use. Scand J Clin Lab Invest 50, 455–459, 1990.
Kwant G, Oeseburg B, Zijlstra WG. Reliability of the determination of whole-blood oxygen affinity by means of blood-gas analyzers and multiwavelength oximeters. Clin Chem 35, 773–777, 1989.
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Oeseburg, B., Rolfe, P., Andersen, O.S., Zijlstra, W.G. (1994). Definition and Measurement of Quantities Pertaining to Oxygen in Blood. In: Vaupel, P., Zander, R., Bruley, D.F. (eds) Oxygen Transport to Tissue XV. Advances in Experimental Medicine and Biology, vol 345. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2468-7_122
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