Abstract
In order to characterize the conformational epitope of the group B meningococcal polysaccharide and of the type III group B Streptococcus capsular polysaccharide NMR measurements were done on a wide variety of native and modified polysaccharides and oligosaccharides. Since these saccharides are highly mobile and exist as random coils in solution, the analysis of the NMR data and molecular modeling was done to take into account this inherent flexibility. The conformational model of extended high-order helices being selected upon binding to a protein, although still hypothetical at this stage, has proven useful in explaining the serology for the conformational epitopes for polysaccharides of group B Neisseria meningitidis, group B Streptococcus type III and Streptococcus pneumoniae type 14.
Article FootNote
Corresponding author: Jean-Robert Brisson. Fax: (613) 952-9092.E-mail: jean-robert.brisson@nrc.ca
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
H.J. Jennings, E. Katzenellenbogen, C. Lugowski, F. Michon, R. Roy, and D.L. Kasper, Structure, conformation, and immunology of sialic acid-containing polysaccharides of human pathogenic bacteria, Pure Appl. Chem. 56:893–905 (1984).
H.J. Jennings, J.R. Brisson, M. Kulakowska, and F. Michon, Polysialic acid vaccines against meningitis caused by Neisseria meningitidis and Escherichia coil K1, in: “Polysialic Acid”, J. Roth et al., eds., Birkhauser Verlag, Basel, pp. 25–38 (1993).
R.A. Pon, L. Lussier, Q.L. Yang, and H.J. Jennings, N-propionylated group B meningococcal polysaccharide mimics a unique bactericidal capsular epitope in group B Neisseria meningitidis, JExp.Med. 185:1929–1938 (1997).
J.R. Brisson, H. Baumann, A. Imberty, S. Perez, and H.J. Jennings, Helical epitope of the group B meningococcal a.(2–8)-linked sialic acid polysaccharide, Biochem. 31:4996–5004 (1992).
R.M. Venable, F. Bizik, T.J. Henderson, and W. Egan, Molecular dynamics simulations of an a-(2–8)-linked sialic acid tetramer in vacuum and solvent, J. Mo1.Struct.(Theochem) 395:375–388 (1997).
S. Bystricky, V. Pavliak, and S.C. Szu, Characterization of colominic acid by circular dichroism and viscosity analysis, Biophys.Chem. 63:147–152 (1997).
M.K. Koenig and R.J. McLean, Influence of metal ions and temperature on the conformation of Escherichia coli K1 capsular polysaccharide, BioMetals 12:47–52 (1999).
D.A. Cumming and J.P. Carver, Virtual and solution conformations of oligosaccharides, Biochem. 26:6664–6676 (1987).
G. Widmalm, R.A. Byrd, and W. Egan, A conformational study of a-L-Rhap-(1–2)-a-L-Rhap-(l-OMe) by NMR nuclear Overhauser effect spectroscopy (NOESY) and molecular dynamics calculations, Carbohydr.Res. 229:195–211 (1992).
S.V. Evans, B.W. Sigurskjold, H.J. Jennings, J.R. Brisson, R. To, W.C. Tse, E. Altman, M. Frosch, C. Weisgerber, H.D. Kratzin, S. Klebert, M. Vaesen, D. Bitter-Suermann, D.R. Rose, N.M. Young, and D.R. Bundle, Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific of a(2–8)- polysialic acid, Biochem. 34:6737–6744 (1995).
H. Baumann, J.R. Brisson, F. Michon, R. Pon, and H.J. Jennings, Comparison of the conformation of the epitope of a(2–8) polysialic acid with its reduced and N-acyl derivatives, Biochem. 32:4007–4013 (1993).
J.R. Brisson, S. Uhrinova, R.J. Woods, M. van der Zwan, H.C. Jarrell, L.C. Paoletti, D.L. Kasper, and H.J. Jennings, NMR and molecular dynamics studies of the type Ill group B Streptococcus capsular polysaccharide and derivatives, Biochem. 36:3278–3292 (1997).
G.H. Weiss, J.E. Kiefer, and J.A. Ferretti, Accuracy and precision in the estimation of internuclear distances for structure determination, J. Magn.Reson. 97:227–234 (1992).
C.A. Laferrière, R.K. Sood, J.-M. de Muys, F. Michon, and H.J. Jennings, Streptococcus pneumoniae type 14 polysaccharide-conjugate vaccines: Length stabilization of opsonophagocytic conformational polysaccharide epitopes., Jnfect.Immun. 66:2441–2446 (1998).
M.R. Wessels and D.L. Kasper, Antibody recognition of the type 14 pneumococcal capsule, J.Exp.Med 169:2121–2131 (1989).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media New York
About this chapter
Cite this chapter
Brisson, JR., Jennings, H.J. (2001). NMR and Molecular Modeling of Complex Carbohydrates and Carbohydrate-Protein Interactions. Applications to Anti-Bacteria Vaccines. In: Wu, A.M. (eds) The Molecular Immunology of Complex Carbohydrates —2. Advances in Experimental Medicine and Biology, vol 491. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1267-7_36
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1267-7_36
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5469-7
Online ISBN: 978-1-4615-1267-7
eBook Packages: Springer Book Archive