Abstract
Trichinella spiralis is a parasitic nematode causing trichinellosis in mammals. The muscle larvae develop from the newborn larvae, delivered by females after mating in the host small intestine of the adult forms, originating from consumed infected meat. The larval growth in the muscles is completed within 20 days post infection. Therefore it was surprising to find high thymidylate synthase (TS; EC 2.1.1.45) activity in non-growing T. spiralis muscle larvae, isolated up to two years post infection (1), as expression of activity of this enzyme (catalyzing the C(5) methylation of dump, yielding dTMP) has been correlated with cell proliferation (2). In order to learn more about regulation of thymidylate synthase expression in the parasite, molecular cloning of T. spiralis thymidylate synthase gene was performed, and expression of the corresponding mRNA studied.
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Dabrowska, M., Jagielska, E., Plucienniczak, A., Kwiatowski, J., Rode, W. (2002). Gene Structure and Expression of Trichinella Spiralis Thymidylate Synthase. In: Milstien, S., Kapatos, G., Levine, R.A., Shane, B. (eds) Chemistry and Biology of Pteridines and Folates. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0945-5_85
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DOI: https://doi.org/10.1007/978-1-4615-0945-5_85
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