Abstract
A HPLC method was developed for the quantitation of tryptophan and its metabolites, in total sixteen compounds, belonging both to the indolyl and kynurenine pathways. Primary amino group containing metabolites have been quantitated as their ophthaldialdehyde/3-mercaptopropionic acid derivatives (5-hydroxytryptophan, tryptophan, 5-hydroxytryptamine, tryptamine), others without derivatization (quinolinic-, nicotinic-, antranilic-, xanthurenic-, kynurenic, indoleacetic-and indolepropionic acids, nicotineamide, melatonin, tryptophol, indole, methyl-indole), in a single run, within 20 min Fluorescence and UV detections were performed simultaneously.
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Presits, P., Molnar-Perl, I. (2003). HPLC of Tryptophan and Its Metabolites: as Opa Derivatives and on the Basis of Their Uv and Fluorescence Spectra, Simultaneously. In: Allegri, G., Costa, C.V.L., Ragazzi, E., Steinhart, H., Varesio, L. (eds) Developments in Tryptophan and Serotonin Metabolism. Advances in Experimental Medicine and Biology, vol 527. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0135-0_82
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DOI: https://doi.org/10.1007/978-1-4615-0135-0_82
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