Abstract
Magnetic resonance imaging (MRI) of small animals has emerged as a valuable tool to noninvasively monitor tumor growth in mouse models of cancer. However, imaging of metastases in mouse models is difficult due to the need for high spatial resolution. We have demonstrated MRI of metastases in the liver, brain, adrenal glands, and lymph nodes in different xenograft mouse models of cancer. MRI of mice was performed with a clinical 3.0 T magnetic resonance scanner and a commercially available small-animal receiver coil. The imaging protocol consisted of T1- and T2-weighted fat-saturated spin echo sequences with a spatial resolution of 200 μm × 200 μm × 500 μm. Total acquisition time was 30 min per mouse. The technique allowed for repetitive examinations of larger animal cohorts to observe the development of metastases.
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References
Damadian R (1971) Tumor detection by nuclear magnetic resonance. Science 171:1151–1153
Barentsz J, Takahashi S, Oyen W et al (2006) Commonly used imaging techniques for diagnosis and staging. J Clin Oncol 24:3234–3244
Schmidt GP, Schoenberg SO, Reiser MF, Baur-Melnyk A (2005) Whole-body MR imaging of bone marrow. Eur J Radiol 55:33–40
Harisinghani MG, Barentsz J, Hahn PF et al (2003) Noninvasive detection of clinically occult lymph-node metastases in prostate cancer. N Engl J Med 348:2491–2499
Koo V, Hamilton PW, Williamson K (2006) Non-invasive in vivo imaging in small animal research. Cell Oncol 28:127–139
Poirier-Quinot M, Ginefri JC, Girard O, Robert P, Darrasse L (2008) Performance of a miniature high-temperature superconducting (HTS) surface coil for in vivo microimaging of the mouse in a standard 1.5T clinical whole-body scanner. Magn Reson Med 60:917–927
Seierstad T, Roe K, Hovik B (2007) Construction of a modified capacitive overlap MR coil for imaging of small animals and objects in a clinical whole-body scanner. Phys Med Biol 52:N513–N522
Xu S, Gade TP, Matei C et al (2003) In vivo multiple-mouse imaging at 1.5 T. Magn Reson Med 49:551–557
Linn J, Schwarz F, Schichor C, Wiesmann M (2007) Cranial MRI of small rodents using a clinical MR scanner. Methods 43:2–11
Inderbitzin D, Stoupis C, Sidler D, Gass M, Candinas D (2007) Abdominal magnetic resonance imaging in small rodents using a clinical 1.5 T MR scanner. Methods 43:46–53
Garbow JR, Wang M, Wang Y, Lubet RA, You M (2008) Quantitative monitoring of adenocarcinoma development in rodents by magnetic resonance imaging. Clin Cancer Res 14:1363–1367
Lyons SK (2005) Advances in imaging mouse tumour models in vivo. J Pathol 205: 194–205
Ikehira H, Yamane T, Fukuda N et al (1988) Fundamental tumor perfusion analysis with nuclear magnetic resonance imaging using gadolinium-diethylene triamine pentaacetic acid. Am J Physiol Imaging 3:7–9
Checkley D, Tessier JJ, Kendrew J, Waterton JC, Wedge SR (2003) Use of dynamic contrast-enhanced MRI to evaluate acute treatment with ZD6474, a VEGF signalling inhibitor, in PC-3 prostate tumours. Br J Cancer 89: 1889–1895
Gauvain KM, Garbow JR, Song SK, Hirbe AC, Weilbaecher K (2005) MRI detection of early bone metastases in b16 mouse melanoma models. Clin Exp Metastasis 22:403–411
Brandsma D, Taphoorn MJ, Reijneveld JC et al (2004) MR imaging of mouse leptomeningeal metastases. J Neurooncol 68:123–130
Thies A, Peldschus K, Ittrich H et al (2009) Magnetic resonance imaging of melanoma metastases in a clinical relevant human melanoma xenograft scid mouse model. Cancer Lett 274:194–200
Weber MH, Sharp JC, Latta P, Hassard TH, Orr FW (2007) Early detection and quantification of murine melanoma bone metastases with magnetic resonance imaging. Skeletal Radiol 36:659–666
Kalber TL, Waterton JC, Griffiths JR, Ryan AJ, Robinson SP (2008) Longitudinal in vivo susceptibility contrast MRI measurements of LS174T colorectal liver metastasis in nude mice. J Magn Reson Imaging 28:1451–1458
Koyama Y, Talanov VS, Bernardo M et al (2007) A dendrimer-based nanosized contrast agent dual-labeled for magnetic resonance and optical fluorescence imaging to localize the sentinel lymph node in mice. J Magn Reson Imaging 25:866–871
Acknowledgements
The authors would like to thank Professor Udo Schumacher, Dr. Anka Thies, and Dr. Daniel Benten for providing mouse models with metastases and helpful discussions. Furthermore, Johannes Salamon is acknowledged for technical assistance and image processing.
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© 2014 Springer Science+Business Media, New York
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Peldschus, K., Ittrich, H. (2014). Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of Cancer. In: Dwek, M., Schumacher, U., Brooks, S. (eds) Metastasis Research Protocols. Methods in Molecular Biology, vol 1070. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-8244-4_16
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DOI: https://doi.org/10.1007/978-1-4614-8244-4_16
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