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Building a Two-Photon Microscope Using a Laser Scanning Confocal Architecture

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Methods in Cellular Imaging

Part of the book series: Methods in Physiology ((METHPHYS))

Abstract

Although conceived over 20 years ago (Gannaway and Sheppard, 1978; Sheppard and Kompfner, 1978) and developed in its modern form 10 years ago (Denk et al., 1990), two-photon excitation (TPE) fluorescence microscopy can be considered a comparatively young technique in far-field fluorescence optical microscopy. This technique has advantages over both widefield and confocal laser scanning microscopy (Wilson and Sheppard, 1984; Wilson, 1990; Pawley, 1995; Webb, 1996; see also Chapter 5) for the study of the three-dimensional (3D) structure and dynamic properties of biological systems (Denk et al., 1995; Hell, 1996; Denk, 1996; Centonze and White, 1998; Diaspro, 1999a,b).

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Authors
  1. Alberto Diaspro
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Editors and Affiliations

  1. W. M. Keck Center for Cellular Imaging, University of Virginia, Charlottesville, Virginia, USA

    Ammasi Periasamy Ph.D. (Director, Professor of Biology and Biomedical Engineering) (Director, Professor of Biology and Biomedical Engineering)

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© 2001 American Physiological Society

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Diaspro, A. (2001). Building a Two-Photon Microscope Using a Laser Scanning Confocal Architecture. In: Periasamy, A. (eds) Methods in Cellular Imaging. Methods in Physiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7513-2_10

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  • DOI: https://doi.org/10.1007/978-1-4614-7513-2_10

  • Publisher Name: Springer, New York, NY

  • Online ISBN: 978-1-4614-7513-2

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