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Deadenylation and P-Bodies

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Ten Years of Progress in GW/P Body Research

Part of the book series: Advances in Experimental Medicine and Biology ((volume 768))

Abstract

Deadenylation is the major step in triggering mRNA decay and results in mRNA translation inhibition in eukaryotic cells. Therefore, it is plausible that deadenylation also induces the mRNP remodeling required for formation of GW bodies or RNA processing bodies (P-bodies), which harbor translationally silenced mRNPs. In this chapter, we discuss several examples to illustrate the roles of deadenylation in regulating gene expression. We highlight several lines of evidence indicating that even though non-translatable mRNPs may be prepared and/or assembled into P-bodies in different ways, deadenylation is always a necessary, and perhaps the earliest, step in mRNA decay pathways that enable mRNP remodeling required for P-body formation. Thus, deadenylation and the participating deadenylases are not simply required for preparing mRNA substrates; they play an indispensable role both structurally and functionally in P-body formation and regulation.

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Acknowledgments

We thank many colleagues who have contributed in various ways over the years to the content described in this chapter, and to Drs. Richard Kulmacz and Julia Lever for critical reading of the manuscript. Work in our laboratory was supported by the U.S. National Institutes of Health, the Houston Endowment, Inc., and the Sandler Program for Asthma Research.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, The University of Texas Medical School, Houston, TX, 77030, USA

    Chyi-Ying A. Chen & Ann-Bin Shyu

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  1. Chyi-Ying A. Chen
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  2. Ann-Bin Shyu
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Correspondence to Ann-Bin Shyu .

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Editors and Affiliations

  1. College of Dentistry, Department of Oral Biology, University of Florida, Southwest Archer Road 1600, Gainesville, 32610, Florida, USA

    Edward K. L. Chan

  2. Dept. Medicine & Medical Biochemistry, University of Calgary, Hospital Drive NW., 3330, Calgary, T2N 4N1, Alberta, Canada

    Marvin J. Fritzler

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Chen, CY.A., Shyu, AB. (2013). Deadenylation and P-Bodies. In: Chan, E., Fritzler, M. (eds) Ten Years of Progress in GW/P Body Research. Advances in Experimental Medicine and Biology, vol 768. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-5107-5_11

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