Abstract
Mere binding of a pore forming toxin’s monomer to the cell surface is not anticipated to modulate cellular signalling. We have shown earlier that the binding of H35N, a membrane bound monomer of α-HL, to the host cell arrests the dynamics of caveolae at the cell surface and initiate the membrane repair mechanism involving caspase-1-dependent activation of sterol regulatory element-binding protein-1. Here, we show that the process of membrane repair induced by H35N involved a decrease in cytosolic potassium, activation of sterol regulatory element-binding protein-1 and -2, which in turn promote cell survival. Moreover, arrest of the dynamics of caveolae by silencing of the kinases involved in kiss and run dynamics of the caveolae also lead to activation of SREBP-1 proving that the loss of dynamics of caveolae may act as sensor for the target cell to activate the repair process. Our data reveals that membrane repair pathway involves sensing of loss of dynamics of caveolae
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Acknowledgments
The authors thank G. C. Mishra for critical support and comments, Mr. Anil Lotke and Ms. Ashwin Atre for technical assistance. SS. is recipient of senior research fellowship from UGC. The financial assistance was provided by the Department of Biotechnology, Government of India.
Financial Assistance The financial assistance for the work was provided by Department of Biotechnology, Government of India, India.
Competing Interests None of the authors have any competing parallel interests.
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Srivastava, S.S., Krishnasastry, M.V. (2012). Cell Membrane Repair Pathway Involves Sensing of Dynamics of Caveolae and Caspase-1. In: Sudhakaran, P., Surolia, A. (eds) Biochemical Roles of Eukaryotic Cell Surface Macromolecules. Advances in Experimental Medicine and Biology, vol 749. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3381-1_9
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DOI: https://doi.org/10.1007/978-1-4614-3381-1_9
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