Abstract
ATM (gene mutated in ataxia-telangiectasia) is a critical central component of the pleiotropic responses of cells to ionizing radiation-induced stress. To gain insight into molecular mechanisms and to enhance our understanding of ATM functions, we have advanced a human model cell system, derived from genetically defined immortal fibroblasts, and we have applied high-throughput genomic, proteomic and metabolomic technologies for a systems level analysis. The cellular characterizations reported here provide the background for application of a systems analysis to integrate transcription, post-translational modifications and metabolic activity induced by exposure of cells to ionizing radiation. We present here a summary of the derivation and characterization of cells comprising this model cell system and review applications of this model to systems analysis of ATM functions.
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Acknowledgments
This work was supported by NIH grant P01CA74175 and the CCSG grant NIH P30 CA51008 to the Lombardi Comprehensive Cancer Center supporting the Proteomics and Metabolomics Shared Resource.
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Jung, M., Timofeeva, O., Cheema, A.K., Varghese, R., Ressom, H., Dritschilo, A. (2011). Human Fibroblasts for Large-Scale “Omics” Investigations of ATM Gene Function. In: Rhim, J., Kremer, R. (eds) Human Cell Transformation. Advances in Experimental Medicine and Biology, vol 720. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0254-1_15
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DOI: https://doi.org/10.1007/978-1-4614-0254-1_15
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