Abstract
The sodium-independent binding of GABA to certain membrane preparations from rat brain exhibits many of the properties of the interaction of GABA with postsynaptic receptors anticipated from in vivo studies (see papers by Enna and Olsen, this meeting). Enna and Snyder (1975) found that, while sodium-independent GABA binding could be detected in homogenates of fresh brain tissue, freezing and thawing the membrane preparation led to a 2-fold increase in observed sodium-independent binding. Subsequently these authors reported that treatment of the membranes with 0.05% Triton X-100 further increased binding up to 5-fold, and also increased the apparent potencies of various GABA agonists (Enna and Snyder, 1977). Wong and Horng (1977) reported that treatment with 0.5% Triton X-100 increased both the apparent affinity and the apparent density of sodium-independent GABA binding from the values obtained using membranes prepared by extensive freezing and thawing.
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References
Abood, L.G., and Hoss, W., 1975, Stereospecific morphine adsorption to phosphatidyl serine and other membranous components of brain, Europ. J. Pharmac. 32: 66–75.
Abood, L.G., Salem, N., Macneil, M., Bloom, L., and Abood, M.E., 1977, Enhancement of opiate binding by various molecular forms of phosphatidylserine and inhibition by other unsaturated lipids, Biochim. Biophys. Acta. 468: 51–62.
Abood, L.G., and Takeda, F., 1976, Enhancement of stereospecific opiate binding to neural membranes by phosphatidylserine, Europ. J. Pharmac. 39: 71–77.
Bird, E.D., and Iversen, L.L., 1973, Huntington’s chorea. Postmortem measurement of glutamic acid decarboxylase, choline acetyltransferase and dopamine in basal ganglia, Brain 97: 457–472.
Bloom, F.E., and Aghajanian, G.K., 1968, Fine structural and cytochemical analysis of the staining of synaptical junctions with phosphotungstic acid, J. Ultrastruct. Res. 22: 361–375.
Cotman, C.W., Levy, W., Banker, G., and Taylor, D., 1971, An ultrastructural and chemical analysis of the effect of Triton X-100 on synaptic plasma membranes, Biochim. Biophys. Acta 249: 406–418.
Cuthbert, A.W., 1967, Membrane lipids and drug action, Pharmac Rev. 19: 59–106.
Davis, G.A., and Bloom, F.E., 1973, Isolation of synaptic junctional complexes from rat brain, Brain Res. 62: 135–153.
Elliott, K.A.C., and Van Gelder, N.M., 1958, Occlusion and metabolism of γ-aminobutyric acid by brain tissue, J. Neurochem. 3: 28–40.
Enna, S.J., Bennett, J.P., Bylund, D.B., Snyder, S.H., Bird, E.D., and Iversen, L.L., 1976, Alterations of brain neurotransmitter receptor binding in Huntington’s chorea, Brain Res. 116: 531–537.
Enna, S.J., and Snyder, S.H., 1975, Properties of γ-aminobutyric acid (GABA) receptor binding in rat brain synaptic membrane fractions, Brain Res. 100: 81–97.
Enna, S.J., and Snyder, S. H., 1976, A simple, sensitive and specific radioreceptor assay for endogenous GABA in brain tissue, J. Neurochem. 26: 221–224.
Enna, S.J., and Snyder, S.H., 1977, Influences of ions, enzymes and detergents on GABA receptor binding in synaptic membranes of rat brain, Molec. Pharmac. 13: 442–45 3.
Fiszer, S., and De Robertis, E., 1967, Action of Triton X-100 on ultrastructure and membrane-bound enzymes of isolated nerve endings from rat brain, Brain Res. 5: 31–44.
Formby, B., 1967, The binding of noradrenaline to phosphatidyl-serine. In vitro studies of an artificial model system of biological origin, Molec. Pharmac. 3: 284–289.
Friedel, R.O., Slotnick, R.N., and Bombardt, P.A., 1977, Effects of γ-aminobutyric acid on 33Pi incorporation into rat brain phospholipids in vivo, Life Sciences 20: 235–242.
Giambalvo, C., and Rosenberg, P., 1976, The effect of phospholipase and proteases on the binding of γ-aminobutyric acid to junctional complexes of rat cerebellum, Biochim. Biophys. Acta, 436: 741–756.
Izumi, F., and Freed, S., 1974, Binding of acetylcholine and cholinergic drugs to proteolipid fractions from rat cerebral cortex and to phospholipids from bovine brain, FEBS Letters 42: 319–322.
Koidi, B., and Florey, E., 1975, Factor I and GABA: resolution of a long-standing problem, Comp. Biochem. Physiol. 51C: 13–23.
Perry, T.L., Hansen, S., and Kloster, M., 1973, Huntington’s chorea deficiency of γ-aminobutyric acid in brain, , New Eng. J. Med. 288: 337–342.
Snodgrass, S.R., and Iversen, L.L., 1973, A sensitive double isotope derivative assay to measure release of amino acids from brain in vitro. Nature New Biol. 241: 154–156.
Watkins, J.C., 1965, Pharmacological receptors and general permeability phenomena of cell membranes, J. Theoret. Biol. 9: 37–50.
Wong, D.T., and Horng, J.S., 1977, Na -independent binding of GABA to the Triton X-100 treated synaptic membranes from cerebellum of rat brain, Life Sciences 20: 445–452.
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© 1978 Plenum Press, New York
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Johnston, G.A.R., Kennedy, S.M.E. (1978). GABA Receptors and Phospholipids. In: Fonnum, F. (eds) Amino Acids as Chemical Transmitters. NATO Advanced Study Institutes Series, vol 16. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4030-0_40
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DOI: https://doi.org/10.1007/978-1-4613-4030-0_40
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