Abstract
The purpose of this chapter is to discuss mechanisms for the transfer of ions across biomembranes which do not result in any net transfer of electrical charges. To begin with, we consider the measurement of current flow across membranes, in order to show that some ion flows are not detected by this measurement technique. Then, we develop the physical-chemical ideas behind the formation of ion pairs, and discuss several examples relevant to ion fluxes across red blood cells. No attempt has been made to consider all examples of electrically neutral exchanges that take place in biomembranes; rather, attention has been focused on principles and examples in erythrocytes.
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References
Bjerrum, N. 1926. Studier over Kromiklorid. Kgl. Danske Videnskab. Selskab. 4: 1 – 123.
Bockris, J. O. M., and A. K. N. Reddy. 1973. Modern Electrochemistry. Plenum, New York. pp. 251 – 267.
Arrhenius, S. 1887. Über die Dissociation der in Wasser gelösten Stoffe. Z. Phys. Chem. 1: 631 – 648.
Handbook of Chemistry and Physics. 1975. 56th ed. CRC Press, Cleveland, Ohio. p. 152.
Garrels, R. M., M. E. Thompson, and R. Siever. 1961. Control of carbonate solubility by carbonate complexes. Am. J. Sei. 259: 24 – 45.
Garrels, R. M., and M. E. Thompson. 1962. A chemical model for sea water at 25°C and one atmosphere total pressure. Am. J. Sci. 260: 57 – 66.
Funder, J., and J. O. Wieth. 1967. Effects of some monovalent anions on fluxes of Na and K and on glucose metabolism of ouabain treated human red cells. Acta Physiol. Scan. 71: 168 – 185.
Funder, J., and J. O. Wieth. 1974. Human red cell sodium and potassium in metabolic alkalosis. Scand. J. Clin. Lab. Invest. 34: 49 – 59.
Funder, J., and J. O. Wieth. 1974. Combined effects of digitalis therapy and of plasma bicarbonate on human red cell sodium and potassium. Scand. J. Clin. Lab. Invest. 34: 153 – 160.
Schatzmann, H. J. 1953. Herzglykoside als Hemmstoffe für den Aktiven kalium und natrium Transport durch die Erythrocytenmambran. Helv. Physiol. Pharmacol. Acta 11: 346 – 354.
Weith, J. O., M. Dalmark, R. B. Gunn, and D. C. Tosteson. 1973. The transfer of monovalent inorganic anions through the red cell membrane. In: Erythrocytes, Thrombocytes, Leukocytes. E. Gerlach, K. Moser, E. Deutsch, and W. Wilmanns, eds. Thieme, Stuttgart, pp. 71 – 76.
Dalmark, M., and J. O. Wieth. 1972. Temperature dependence of chloride, bromide, iodide, thiocyanate, and salicylate transport in human red cells. J. Physiol. (Lond.) 224: 553 – 610.
Dalmark, M. 1976. Effects of halides and bicarbonate on chloride transport in human red blood cells. J. Gen. Physiol. 67: 223 – 234.
Kaplan, J., and H. Passow. 1974. Effects of phlorizin on net chloride movements across the valinomycin- treated erythrocyte membrane. J. Membr. Biol. 19: 179 – 194.
Tosteson, D. C., and J. F. Hoffman. 1960. Regulation ol cell volume by active cation transport in high and low potassium sheep red cells. J. Gen. Physiol. 44: 169 – 194.
Davis, J. Personal communication.
Haas, M., J. M. Schooler, and D. C. Tosteson. 1975. Coupling of lithium to sodium transport in human red cells. Nature 258: 425 – 427.
Davies, C. W. 1962. Ion Association. Butterworth, London, p. 169.
Wieth, J. O. 1971. Erythrocytes Selektive Ionpermea- bilitet. FADL’s Forlag, Copenhagen, pp. 157 – 163.
Wieth, J. O. 1970. Effects of monovalent cations on sodium permeability of human red cells. Acta Physiol. Scand. 79: 76 – 87.
Sillen, L. G. 1971. Stability Constants of Metal-Ion Complexes. Special Publication No. 25 and No. 17. The Chemical Society, Burlington House, London.
McCorkell, R. H., M. M. Sein, and J. W. Irvine, Jr. 1968. Extraction of HMX4 acids by solvents of high dielectric constant. J. Inorg. Nucl. Chem. 30:1155– 1160.
Koskikallio, J., and S. Syijapalo. 1965. Association of mineral acids in water with dioxane-water mixtures. Acta Chem. Scand. 19: 429 – 437.
Gunn, R. B., J. O. Wieth, and D. C. Tosteson. 1975. Some effects of low pH on chloride exchange in human red blood cells. J. Gen. Physiol 65: 731 – 749.
Brazy, P. C., and R. B. Gunn. Unpublished observations.
Parsegian, A. 1969. Energy of an ion crossing a low dielectric membrane. Solutions to four relevant electrostatic problems. Nature 221: 844 – 846.
Tosteson, D. C. 1959. Halide transport in red blood cells. Acta Physiol. Scand. 46: 19 – 41.
Brahm, J. 1975. Chloride permeability in human red cells at 0–38°C. Fifth International Biophysics Congress, Copenhagen, p. 319 (Abstract).
Gunn, R. B., M. Dalmark, D. C. Tosteson, and J. O. Wieth. 1973. Characteristics of chloride transport in human red blood cells. J. Gen. Physiol. 61: 185 – 206.
Brahm, J. 1977. Temperature dependent changes of chloride transport kinetics in human red cells. J. Gen. Physiol. 70: 283 – 306.
Deuticke, B., and E. Gerlach. 1967. Beeinflussung von Form und Phosphat-Permeabilität menschlichen Erythrocytes durch Hämolysine, Benzol-Derivate und pharmakologisch aktive Substanzen. Klin. Wochenschr. 45: 977 – 983.
Gunn, R. B., and D. C. Tosteson. 1976. The effect of 2,4,6-trinitro-m-cresol on cation and anion transport in sheep red blood cells. J. Gen. Physiol. 57: 593 – 609.
Gunn, R. B., and J. A. Cooper. 1975. Effect of local anesthetics on chloride transport in erythrocytes. J. Membr. Biol 25: 311 – 326.
Brazy, P. C., and R. B. Gunn. 1975. Furosemide inhibition of chloride transport in human red blood cells. Physiologist 18:151 (Abstr.).
Cousin, J. L., and R. Motais. 1976. The role of carbonic anhydrase inhibitors on anion permeability into ox red blood cells. J. Physiol (Lond.) 256: 61 – 80.
Gunn, R. B., and R. G. Kirk. 1976. Anion transport and membrane morphology. J. Membr. Biol 27:265– 282.
Hunter, M. J. 1971. A quantitative estimate of the non- exchange restricted chloride permeability of the human red cell. J. Physiol 218: 49P – 50 P.
Tosteson, D. C., R. B. Gunn, and J. O. Wieth. 1973. Chloride and hydroxyl ion conductance of sheep red cell membranes. In: Erythrocytes, Thrombocytes, Leukocytes. E. Gerlach, K. Moser, E. Deutsch, and W. Wilmanns, eds. Thieme, Stuttgart, pp. 62 – 66.
Lassen, U. V., and O. Sten-Knudsen. 1968. Direct measurements of membrane potential and membrane resistance of human red cells. J. Physiol (Lond.) 195: 681 – 696.
Lassen, U. V. 1972. Membrane potential and membrane resistance of red cells. In: Oxygen Affinity of Hemoglobin and Red Cell Acid-Base Status. M. Rorth and P. Astrup, eds. Munksgaard, Copenhagen, pp. 291 – 304.
Stoner, L. C., and F. M. Kregenow. 1976. Chloride fluxes and voltage measurements in single red blood cells. Biophys. J. 16: 170a.
Gunn, R. B. Unpublished observations.
Passow, H. 1969. Passive ion permeability of the erythrocyte membrane. Prog. Biophys. 19: 424 – 446.
Cabantchik, Z. I., and A. Rothstein. 1974. Membrane proteins related to anion permeability of human red blood cells. I. Localization of disulfonic stilbene binding sites in proteins involved in permeation. J. Membr. Biol 15: 207 – 226.
Lepke, S., H. Fasold, M. Pring, and H. Passow. 1976. A study of the relationship between inhibition of anion exchange and binding to the red blood cell membrane of 4′,4′-diisothiocyanostilbene-2,2′-disulfonic acids (DIDS) and its dihydro derivative (H2DIDS). J. Membr. Biol 29: 147 – 177.
Rothstein, A., Z. I. Cabantchik, M. Balshin, and R. Juliano. 1975. Enhancement of anion permeability in lecithin vesicles by hydrophobic proteins extracted from red blood cell membranes. Biochem. Biophys. Res. Commun. 64: 144 – 150.
Gunn, R. B. 1972. A titratable carrier model for both mono- and divalent anion transport in human red blood cells. In: Oxygen Affinity of Hemoglobin and Red Cell Acid-Base Status. M. Rorth and P. Astrup, eds. Munksgaard, Copenhagen, pp. 823 – 827.
Bretscher, M. S. 1971. A major protein which spans the human erythrocyte membrane. J. Mol Biol 59:351– 357.
Steck, T. L. 1974. The organization of proteins in the human red blood cell membrane. A review. J. Cell Biol 62: 1 – 19.
Nicolson, G. L., and S. J. Singer. 1971. Ferritin-conjugated plant agglutinins as specific saccharide stains for electron microscopy: Application to saccharides bound to cell membranes. Proc. Natl Acad. Sci. U.S.A. 68: 942 – 945.
Patlak, C. S. 1957. Contributions to the theory of active transport. II. The gate type non-carrier mechanism and generalizations concerning tracer flow, efficiency, and measurement of energy expenditure. Bull Math. Biophys. 19: 209 – 235.
Duhm, J. and B. F. Becker. 1978. Studies on Na+- dependent Li+ countertransport and bicarbonate-stimulated Li+ transport in human erythrocytes. In: Drugs, Hormones, and Membranes. L. Bolis, J. F. Hoffman, and R. W. Staub, eds. Raven Press, N.Y. In press.
Funder, J., and D. C. Tosteson. 1977. Effects of bicarbonate on lithium transport in human red cells. Proc. Intl. Union. Physiol Sci. 13:248, Abstract 724 (Paris).
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Gunn, R.B. (1978). Electrically Neutral Ion Transport in Biomembranes. In: Andreoli, T.E., Hoffman, J.F., Fanestil, D.D. (eds) Physiology of Membrane Disorders. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3958-8_13
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DOI: https://doi.org/10.1007/978-1-4613-3958-8_13
Publisher Name: Springer, Boston, MA
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